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논물의 미생물군으로부터 해조분해능을 갖는 균주의 분리

Isolation of a Seaweed Hydrolytic Strain from the Microflora in Water of a Paddy Field

  • 김해섭 (여수대학교 식품공영양학부) ;
  • 최옥수 (순천제일대학 식생활학부) ;
  • 강동수 (여수대학교 식품공영양학부) ;
  • 박욱민 (여수대학교 식품공영양학부) ;
  • 백승한 (순천제일대학 식생활학부) ;
  • 배태진 (여수대학교 식품공영양학부)
  • 발행 : 2004.02.01

초록

해조 또는 해조성분을 가수분해시키는 효소를 생산하는 미생물이 존재할 것으로 추정되는 논물에서 채취한 미생물군 시료에서 다시마분말 첨가 고체배지를 이용하여 순수배양을 실시하여 16균주를 분리하였으며, 이들 균주를 다시마분말 첨가 액체배지에서 3주간 배양하며, 1주 간격으로 전당과 환원당의 함량을 측정하여 추출율을 비교한 결과 배양 전 기간에서 30A412균주가 높은 추출율을 나타내었다. 30A412 균주를 제외한 나머지 15균주는 3주간의 배양으로도 그 추출율이 10%이하를 나타내었다. 30A412균주의 알긴산, 미역 및 다시마 분말 첨가 배지에서의 성장율은 배양 1주일 후부터 서서히 증가하였으며, 대조구보다 높은 성장율을 보였다. 알긴산, 다시마 및 미역 분해율은 배양기간이 경과함에 따라 증가하는 경향을 보였다. 균체 성장율과 분해율 모두 다시마 배지에서 가장 높은 값을 나타내었으며, 다음으로 미역배지, 알긴산 배지의 순이었다.

Various bacterial strains were isolated from water in a paddy field, and their seaweed degrading activities were investigated. As the result, 16 strains were obtained from the microflora sample. They were incubated in a liquid medium of sea tangle powder for 3 weeks. Ratios of reduced sugar to total sugar of the liquid medium were measured once a week during the incubation period. Ratio of reduced sugar to total sugar of 30A412 strain was highest. Accordingly, 30A412 strain was incubated in three different liquid media of sodium alginate, sea tangle powder, and sea mustard powder for 3 or 4 weeks. The ratios of reduced sugar to total sugar and cell growth were measured once a week during the incubation period. Ratios of reduced sugar to total sugar and cell growth were increased with the incubation period. Cell growth and degrading ratio were highest in the liquid medium with sea mustard powder.

키워드

참고문헌

  1. Bull. of Jap. Soc. Sci. Fish. v.27 Decomposition of alginic acid by microorganisms-Ⅳ. On the Vibrio-type bacteria, newly isolated from the decaying Laminaria Ando,Y.;K.Inoue https://doi.org/10.2331/suisan.27.339
  2. Bull. of Jap. Soc. Sci. Fish. v.27 Decomposition of alginic acid by microorganisms-Ⅴ. On the alginase of Vibrio sp. SO-20 strain Ando,Y.;K.Inoue https://doi.org/10.2331/suisan.27.342
  3. Bull. of Jap. Soc. Sci. Fish. v.31 Decomposition of alginic acid by microorganisms-Ⅴ. On the modes of action of two alginases Ando,Y.;K.Inoue https://doi.org/10.2331/suisan.31.552
  4. Carbohydrate Research v.57 Isolation of a poly-a-L-guluronate lyase from Klebsiella aerogenes Boyd,J.;J.R.Turvey https://doi.org/10.1016/S0008-6215(00)81928-X
  5. Carbohydrate Research v.66 Structural studies of alginic acid, using a bacterial poly-a-L-guluronate lyase Boyd,J.;J.R.Turvey https://doi.org/10.1016/S0008-6215(00)83251-6
  6. Korean. J. Food Sci. Technol. v.6 Effectiveness of gammairradiation of the extraction of algal polysaccharides Cho,H.O.;S.R.Lee
  7. Biochem. J. v.159 Purification and properties of an alginate lyase from a marine bacterium Davidson,I.W.;I.W.Sutherland;C.J.Lawson
  8. J. of Bacteriology v.80 Studies on bacterial utilization of uronic acid. Ⅳ. Alginolytic and mannuronic acid oxidizing isolates Eller,J.;W.J.Payne
  9. Biochimica et Biophysica Acta. v.358 Isolation and certain preperties of alginate lyase Ⅵ from the Mollusk Littorina sp Elyakova,L.A.;V.V.Favorov https://doi.org/10.1016/0005-2744(74)90464-1
  10. Science v.129 Alginase in the sea urchin Strongylocentrotus purpuratus Eppley,R.W.;R.Lasker https://doi.org/10.1126/science.129.3343.214
  11. Can. J. Microbiol. v.23 Enzymatic hydrolysis of agra; purification and characterization of β-neoagarote-traose hydrolase from Pseudomonas atlantica Groleau,D.;W.Yaphe https://doi.org/10.1139/m77-100
  12. J. Fer. and Bioen. v.68 Symbiotic formation of alginate lyase in mixed culture of bacteria isolated from soil Kaneko,Y.;Y.Yonemoto;K.Okayama;A.Kimura;K.Murata
  13. J. of Fermentation & Bioengineering v.70 Bacterial alginate lyase properties of the enzyme formed in a mixed culture bacteria isolated from soil Kaneko,Y.;Y.Yonemoto;K.Okayama;A.Kimura;K.Murata https://doi.org/10.1016/0922-338X(90)90173-T
  14. J. Biochem. v.66 Alginate lyases of Pseudomonas sp Kashiwabara,Y.;S.Hiroshi;K.Nisizawa
  15. J. Korean Fish. Soc. v.35 Studies on the hydrolysis of seaweed using microorganisms and its application, Ⅰ. Screening of microfloras involved in hydrolysis of sea tangle (Laminaria japonica) and sea mustard (Undaria pinnatifida) Kim,H.S.;T.J.Bae https://doi.org/10.5657/kfas.2002.35.4.438
  16. Korean J. Food & Nutr. v.15 Studies on the hydrolysis of seaweed using microorganisms and its application, Ⅱ. Screening of microfloras involved in hydrolysis of seaweed tenella, seaweed fusiforme and green laver Kim,H.S.;T.J.Bae
  17. Nipoon Suisan Gakkaishi v.55 Isolation of bacteria capable of producing alginate0degrading enzyme from natural environment Kitamikado,M.;C.H.Tseng;T.Aoki;K.Yamaguchi;T.Araki https://doi.org/10.2331/suisan.55.709
  18. Korean J. Phycol. v.1 A check list of marine algae in Korea Lea,I.K.;J.W.Kang https://doi.org/10.2216/08-103.1
  19. J. Korean Soc. Food Nutr. v.22 Effect of extraction temperature on some quality of sea tangle extract Lee,J.K.;H.S.Choi;S.K.Yoon;W.J.Kim
  20. J. of Biochemistry v.81 Substrate specificity of endo-polyguluronide lyases from Pseudomonas sp. on the basic of their kinetic properties Min,K.H.;S.F.Sasaki;Y.Kashiwabara;K.Nisizawa
  21. J. of Biochemistry v.81 Multiple components of endo-polygul-uronide lyase of Pseudomonas sp Min,K.H.;S.F.Sasaki;Y.Kashiwabara;K.Nisizawa;K.Nisizawa
  22. J. of Biochemistry v.81 Fine structure of SMG alginate fragment in the light of its degradation by alginate lyases Min,K.H.;S.F.Sasaki;Y.Kashiwabara;K.Nisizawa;K.Nisizawa
  23. Agric. Biol. Chem. v.41 Isolation and properties of alginate lyase from the mid-gut gland of wreath shell Turbo cornutus Muramatsu,T.;S.Hirose;M.Katayose https://doi.org/10.1271/bbb1961.41.1939
  24. J. of Bio. Chem. v.242 Alginic acid degradation by eliminases from abalone hepatopancreas Nakada,H.I.;P.C.Sweeny
  25. J. of Biochemistry v.64 Alginate lyases in the hepatopancreas of a marine mollusc, Dolabella auricula Solander Nisizawa,K.;S.Fujibayashi;Y.Kashiwabara
  26. Nippon Suisan Gakkaishi v.58 Isolation and some properties of alginate lyase from a marine bacterium Vibrio sp. AL-128 Tseng,C.H.;K.Yamaguchi;M.Kitamikado https://doi.org/10.2331/suisan.58.533
  27. Nippon Suisan Gakkaishi v.58 Two types of alginate lyase from a marine bacterium Vibrio sp. AL-9 Tseng,C.H.;K.Yamaguchi;M.Kitamikado https://doi.org/10.2331/suisan.58.743
  28. Nature v.192 A new unsaturated uronide isolated from alginase hydrolysate Tsujino,I.;T.Saito https://doi.org/10.1038/192970a0
  29. Agric. Biol. Chem. v.26 Studies on alginase. Part Ⅱ. A new unsaturated uronide isolated from alginase hydrolysate Tsujino,I.;T.Saito https://doi.org/10.1271/bbb1961.26.115
  30. J. Bacteriol. v.28 Decomposition of polyuronides by fungi and bacteria Waksman,S.A.;M.C.Allen
  31. J. of Ferm. & Bioengineering v.72 Bacterial alginate lyase; characterization of alginate lyase-producing bacteria and purification of the enzyme Yonemoto,Y.;K.Murata;A.Kimura;H.Yamaguchi;K.Okayama https://doi.org/10.1016/0922-338X(91)90208-X
  32. On the Alginic Acid Hydrolyzing Enzyme of Pseudomonas alginoliquefaciens Ⅰ v.1 Yoshikawa,M.
  33. Studies on the Mechanism of Alginase Production by Bacteria (Ⅰ) v.2 Yoshikawa,M.
  34. Studies on the Mechanism of Alginase Production by Bacteria (Part Ⅱ) v.2 Yoshikawa,M.;K.Watanabe