Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Continuous Production of γ-aminobutyric Acid by Immobilization of Lactobacillus brevis

Lactobacillus brevis의 고정화 균체에 의한 γ-aminobutyric acid의 연속 생산

  • 류병호 (경성대학교 식품공학과) ;
  • 전재호 (경성대학교 식품공학과)
  • Published : 2004.02.01
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Abstract

The optimal conditions for the continuous production of $\gamma$-aminobutyric acid by immobilization of Lactobacillus brevis BH-21 using column type reactor were investigated. The optimal conditions of operation were 2.2 mm diameter bead of 3.0% sodium alginate at 10 mL/h of substrate feeding rate. Continuous production by immobililzed cells showed the highest productivity with replacement of fresh medium in every 48h for fourth fermentatoin cycle following the rendition of $\gamma$-aminobutyric acid productivity. A productivity of $\gamma$-aminobutyric acid could be obtained for 25 days by continuous column type reactor under optimal conditions.

Lactobacillus brevis BH-21의 균체를 고정화하여 column형 reactor를 이용하여 $\gamma$-aminobutyric acid의 연속적 생산을 검토하였다. Lactobacillus brevis BH-21에 의한 $\gamma$-aminobutyric acid 생산조건은 고정화 담체로는 3.0% sodium alginate가 좋았고 직경 2.2 mm의 bead를 사용하여 기질의 주입속도는 10 mL/h이 최적 조건이었다. Lactobacillus brevis BH-21의 고정화 균체와 유리 균체를 사용하여 $\gamma$-aminobutyric acid의 생산을 최적 조건하에서 1, 2, 3, 및 4차 발효 동안 48시간마다 신선한 기질을 주입하면서 비교ㆍ조사한 결과 고정화 균체를 사용 시에 생산량이 더 많았다. Column형 reactor에 의한 연속 발효 시 발효 25일까지 생산이 우수하였고 발효 30일까지는 $\gamma$-aminobutyric acid를 생산할 수 있었다.

Keywords

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