Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Antimicrobial Activities and Food Preservative Effects of Agrimoniae Herba

용아초 추출물의 항균력과 식품보존효과

  • 박나영 (대구가톨릭대학교 식품산업학부) ;
  • 박경남 (대구가톨릭대학교 식품산업학부) ;
  • 이신호 (대구가톨릭대학교 식품산업학부)
  • Published : 2004.02.01
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Abstract

Ethanol extract of Agrimoniae Herba showed strong antimicarobial activity againt Listeria monocytogenes, Staphlococcus aureus, Salmonella typhimurium, Escherichia coli and Aeromonas hydrophila subsp. hydrophila The growth of tested organisms was significantly inhibited above 4 log in tryptic soy broth with about 0.12%(w/v) of Agrimoniae Herba after incubation for 12 hrs at 37$^{\circ}C$. Antimicrobial activity of Agrimoniae Herba was decreased by heat treatment at above 8$0^{\circ}C$. But Agrimoniae Herba showed strong inhibitory effect after heat treatment at 121$^{\circ}C$ for 15 min. The growth of L. monocytogenes was inhibited by addition of 0.6% Agrimoniae Herba in food system (ham homogenate).

식품 보존 및 기능성 향상을 위한 천연 첨가제 개발을 위하여 용아초의 식품부패 및 식중독 원인균인 S. aureus, E. coli, S. fyphimurium, L. monocytogene, A. hydropila에 대한 항균활성을 검토하였다. 용아초 에탄올 추출물은 공시균주에 대해 항균활성을 나타내었다. 용아초 추출물의 최종 농도가 0, 0.02, 0.04, 0.06, 0.12, 0.18%(w/v) 로 조절한 TSB 배지에 24시간 배양한 공시 균주를 각각 접종하여 37$^{\circ}C$에서 24시간 배양하면서 생균수를 측정 한 결과 0.04% 첨 가농도로 공시 균주의 성장은 억제되지 않았으며, 0.06% 첨가에 의해 배양 6시간 이후 균주에 따라 상이한 억제현상을 나타내었다. 용아초 추출물 0.12% 첨가에 의해 공시 균주는 대조구에 비해 4log 이상 뚜렷하게 성장이 억제되는 경향을 나타내었다. 용아초 추출물을 8$0^{\circ}C$, 10$0^{\circ}C$에서 30분, 121$^{\circ}C$에서 15분간 열처리 후 항균활성을 검색한 결과 가열 온도가 증가함에 따라 항균활성은 감소하였으나, 121$^{\circ}C$에서 15분간 열처리 후에도 강한 항균활성을 유지하였다. 햄 유화물에 용아초 추출물을 0.6% 농도로 첨가한 경우에 L. monocytogenes에 대한 항균활성을 나타내었다.

Keywords

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