The Plant Pathology Journal

The Korean Society of Plant Pathology (한국식물병리학회)
권호 표지
DOI QR코드

DOI QR Code

Bacillus spp. as Biocontrol Agents of Root Rot and Phytophthora Blight on Ginseng

  • Bae, Yeoung-Seuk (Plant Pathology Division, National Institute of Agricultural Science and Technology, Rural Development Administration) ;
  • Park, Kyungseok (Plant Pathology Division, National Institute of Agricultural Science and Technology, Rural Development Administration) ;
  • Kim, Choong-Hoe (Plant Pathology Division, National Institute of Agricultural Science and Technology, Rural Development Administration)
  • Published : 2004.03.01
Download PDF
⟨ Previous Next ⟩

Abstract

Ginseng (Panax ginseng) is one of the most widely cultivated medicinal herbs in Korea. However, yield losses reached up to 30-60％ due to various diseases during 3 or 5 years of ginseng cultivation in the country. Therefore, successful production of ginseng roots depends primarily on the control of diseases. The objective of this study was to select potential biocontrol agents from rhizobacteria isolated from various plant internal root tissues for the control of multiple ginseng diseases as an alternative to fungicides. Among 106 Bacillus strains, two promising biocontrol agents, Bacillus pumilus strain B1141 and Paenibacillus lentimobus strain B1146, were selected by screening against root rot of ginseng caused by Cylindrocarpon destructans in a greenhouse. Pre-inoculation of selected isolates to seed or l-year-old root of ginseng resulted in stimulation of shoot and/or root growth of seedlings, and successfully controlled root rot caused by C. destructans (P<0.05). Furthermore, drenching of cell suspension of the selected isolates on seedling-growing pots reduced the incidence of Phytophthora blight after the seedlings were challenged with zoospores of Phytophthora cactorum (P<0.05). P. lentimorbus strain B1146 showed antifungal activity against various soil-borne pathogens in vitro, while B. pumilus strain B1141 did not show any. Results of this study suggest that some rhizobacteria can induce resistance against various plant diseases on ginseng.

Keywords

References

  1. Jetiyanon, K. and Kloepper, J. W. 2002. Mixtures of plant growthpromoting rhizobacteria for induction of systemic resistance against multiple plant diseases. Biological Control 24:285-291 https://doi.org/10.1016/S1049-9644(02)00022-1
  2. Joy, A. E. and Parke, J. L. 1995. Biocontrol of Alternaria leaf blight on America ginseng by Burkholderia cepacia AMMD. In: Proceeding of International Ginseng Conference. W.G. Bailey, C. Whitehead, J.T.A. Proctor and J.T. Kyle (eds), Vancouver, Canada. pp. 93-100
  3. Kempe, J. and Sequira, L. 1983. Biological control of bacterial wilt of potatoes: Attempts to induce resistance by treating tubers with bacteria. Plant Dis. 67:499-503 https://doi.org/10.1094/PD-67-499
  4. Liu, L., Kloepper, J. W. and Tuzun, S. 1995a. Induction of systemic resistance in cucumber against Fusarium wilt by plant growth-promoting rhizobacteria. Phytopathology 85:695-698 https://doi.org/10.1094/Phyto-85-695
  5. Liu, L., Kloepper, J. W. and Tuzun, S. 1995b. Induction of systemic resistance in cucumber against angular leaf spot by plant growth-promoting rhizobacteria. Phytopathology 85:843-847 https://doi.org/10.1094/Phyto-85-843
  6. Mahaffee, W. F. and Kloepper, J. W. 1994. Applications of plant growth-promoting rhizobacteria in sustainable agriculture. In: Soil Biota-Management in Sustainable Farming System. C.E. Pankhurst, B.M. Double, V.V.S.R. Gupta and P.R. Grace (eds), CSIRO Adelaide, Australia. pp. 23-31
  7. Mitchell, D. J. and Kannwischer-Mitchell, M. E. 1992. Phytophthora. In: Methods for Research on Soilborne Phytopathogenic Fungi. L.L. Singleton, J.D. Mihail, and C.M. Rush (eds), APS press, St. Paul, Minnesota. pp. 31-38
  8. Nejad, P. and Johnson, P. A. 2000. Endophytic bacteria induce growth promotion in oilseed rape and tomato. Biological Control 18:208-215 https://doi.org/10.1006/bcon.2000.0837
  9. Ohh, S. H. 1986. Diseases of ginseng: environmental and host effect on disease outbreak and growth of pathogens. Korean J. Ginseng Sci. 5:73-84
  10. Ohh, S. H., Yu, Y. H., Kim, K. H. and Cho, D. H. 1992. Studies on control of soil-borne diseases and insects of ginseng and development of antifungal compound. In: Ginseng Cultivation Bul. Korean Ginseng and Tobacco Research Inst. pp. 121-184

Cited by

  1. Biological characteristics ofBacillus amyloliquefaciensAK-0 and suppression of ginseng root rot caused byCylindrocarpon destructans vol.122, pp.1, 2017, https://doi.org/10.1111/jam.13325
  2. Current knowledge and perspectives of Paenibacillus: a review vol.15, pp.1, 2016, https://doi.org/10.1186/s12934-016-0603-7
  3. Biological characteristics of Paenibacillus polymyxa GBR-1 involved in root rot of stored Korean ginseng vol.40, pp.4, 2016, https://doi.org/10.1016/j.jgr.2015.09.003
  4. An investigation of Panax ginseng Meyer growth promotion and the biocontrol potential of antagonistic bacteria against ginseng black spot 2018, https://doi.org/10.1016/j.jgr.2017.03.012
  5. Soil Environment and Soil-borne Plant Pathogen Causing Root Rot Disease of Ginseng vol.45, pp.3, 2012, https://doi.org/10.7745/KJSSF.2012.45.3.370
  6. Biocontrol Efficacies of Bacillus Species Against Cylindrocarpon destructans Causing Ginseng Root Rot vol.27, pp.4, 2011, https://doi.org/10.5423/PPJ.2011.27.4.333
  7. Antagonistic Bacillus species as a biological control of ginseng root rot caused by Fusarium cf. incarnatum vol.38, pp.2, 2014, https://doi.org/10.1016/j.jgr.2013.11.016
  8. Induction of systemic resistance in Panax ginseng against Phytophthora cactorum by native Bacillus amyloliquefaciens HK34 vol.39, pp.3, 2015, https://doi.org/10.1016/j.jgr.2014.12.002
  9. strain (NK3-4) with the potential to control plant diseases vol.61, pp.10, 2018, https://doi.org/10.1139/gen-2018-0113