Antioxidant Activities of Isoflavones from the Rhizomes of Belamcanda chinensis on Carbon Tetrachloride-Induced Hepatic Injury in Rats

  • Jung, Sang-Hoon (Natural Products Research Institute, College of Pharmacy, Seoul National University, Natural Products Research Center, Korea Institute of Science and Technology) ;
  • Lee, Yeon-Sil (Seokwon Life Science Research Institute) ;
  • Lim, Soon-Sung (Silver Biotechnology Research Center, Hallym University) ;
  • Lee, Sanghyun (Natural Products Research Institute, College of Pharmacy, Seoul National University) ;
  • Shin, Kuk-Hyun (Natural Products Research Institute, College of Pharmacy, Seoul National University, Seokwon Life Science Research Institute) ;
  • Kim, Yeong-Shik (Natural Products Research Institute, College of Pharmacy, Seoul National University)
  • 발행 : 2004.02.01

초록

The present study was carried out to clarify whether tectorigenin and tectoridin isolated from the rhizomes of Belamcanda chinensis (Iridaceae) inhibit hepatic damage induced by carbon tetrachloride ($CCl_4$)-intoxication in rats by the experimental methods in vitro and in vivo. Tectorigenin and tectoridin exhibited a significant decrease in serum transaminase activities elevated by hepatic damage induced by $CCl_4$-intoxication in rats, as well as in a lipid peroxidation causing a significant decrease in malondialdehyde (MDA) production by thiobarbituric acid (TBA)-reactant assay. Both compounds also showed strong increase in the antioxidant enzymes such as hepatic cytosolic superoxide dismutase (SOD), catalase and glutathione peroxidase (GSH-px) activities in $CCl_4$-intoxicated rats. These results suggested that tectorigenin and tectoridin isolated from the rhizomes of B. chinensis possess not only the antioxidative, but also the hepatoprotective activities in $CCl_4$ -intoxicated rats.

키워드

참고문헌

  1. Beckman, K. B. and Ames, B. N., The free radical theory of aging matures. Physiol. Rev., 78, 547-581 (1998)
  2. Brar, S. S., Kennedy, T. P., Sturrock, A. B., Huecksteadt, T. P, Quinn, M. T., Murphy, T. M., Chitano, P., and Hoidal, J. R., NADPH oxidase promotes NF-kB activation and proliferation in human airway smooth muscle. Am. J. Physiol. Lung Cell Mol. Physiol., 282, L782-L795 (2002)
  3. Buege, J. A. and Aust, S. D., Microsomal lipid peroxidation. Method Enzymol., 52, 302-306 (1978) https://doi.org/10.1016/S0076-6879(78)52032-6
  4. Burk, R. F., Nishiki, K., Lawrence, R. A, and Chance, B., Peroxide removal by selenium-dependent and selenium-independent glutathione peroxidases in hemoglobin free per-fused rat liver. J. Biol. Chem., 253, 43-46 (1978)
  5. Cross, C. E., Oxygen radicals and human disease. Ann. Intem. Med., 107,526-545 (1987). https://doi.org/10.7326/0003-4819-107-4-526
  6. Ito, H., Onoue, S., and Yoshida, T., Isoflavonoids from Belamcanda chinensis. Chem. Pharm. Bull., 49, 1229-1231 (2001) https://doi.org/10.1248/cpb.49.1229
  7. Jung, S. H., Lee, Y S., Lee, S., Lim, S. S., Kim, Y S., Ohuchi, K., and Shin, K. H., Anti- angiogenic and anti-tumor activities of isoflavonoids from the rhizomes of Belamcanda chinensis. Planta Med., 69, 617-622 (2003) https://doi.org/10.1055/s-2003-41125
  8. Kim, Y. P, Yamada, M., Lim, S. S., Lee, S. H., Ryu, N., Shin, K. H., and Ohuchi, K., Inhibition by tectorigenin and tectoridin of prostaglandin $E_{2}$ production and cyclooxygenase-2 induction in rat peritoneal macrophages. Biochim. Biophys. Acta, 10, 399-407 (1999)
  9. Kovacic, P. and Jacintho, J. D., Mechanisms of carcinogenesis: focus on oxidative stress and electron transfer. Curr. Med. Chem., 8, 773-796 (2001) https://doi.org/10.2174/0929867013373084
  10. Kumaravelu, P., Dakshinamoorthy, D. P., Subramaniam, S., Devaraj, H., and Devaraj, N. S., Effect of eugenol on drug-metabolizing enzymes of carbon tetrachloride-intoxicated rat liver. Biochem. Pharmacol., 49,1 703-1707 (1995)
  11. Kubow, S., Toxicity of dietary lipid peroxidation products. Trends Food Sci. Tech., 1,67-71 (1990) https://doi.org/10.1016/0924-2244(90)90049-5
  12. Lee, K. T., Sohn, I. C., Kim, D. H., Choi, J. W., Kwon, S. H., and Park, H. J., Hypoglycemic and hypolipidemic effects of tecto-rigenin and kaikasaponin III in the streptozotocin-induced diabetic rat and their antioxidant activity in vitro. Arch. Pharm. Res., 23, 461-466 (2000) https://doi.org/10.1007/BF02976573
  13. Lowry, O. H., Posenbrough, N. N., Farr, A. L., and Randall, R. J., Protein measurement with the folin phenol reagent. J. Biol. Chem., 193, 265-275 (1951)
  14. McCord, J. M. and Fridovich, I., Superoxide dismutase: An enzymatic function for erythrocuprein (hemocoprein). J. Biol. Chem., 244, 6049-6055 (1969)
  15. Park, K. Y., Jung, G. O., Choi, J. W., Lee, K. T., and Park, H. J., Potent antimutagenic and their anti-lipid peroxidative effect of kaikasaponin III and tectorigenin from the flower of Pueraria thunbergiana. Arch. Pharm. Res., 25, 320-324 (2002) https://doi.org/10.1007/BF02976633
  16. Pike, J. and Chandra, R. K., Effect of vitamin and trace element supplementation on immune indices in healthy elderly. Int. J. Vitamin Nutr. Res., 65, 117-120 (1995)
  17. Recknagel, R. O., Glende Jr, E. A., Dolak, J. A., and Waller, R. L., Mechanisms of carbon tetrachloride toxicity. Pharmacol. Therapeut., 43, 139-154 (1989) https://doi.org/10.1016/0163-7258(89)90050-8
  18. Reitman, S. and Frankel, S., A chlorotrimetric method for the determination of sGOT and sGPT Am. J. Clin. Pathol., 28, 56-63 (1957)
  19. Rigo, A and Rotilio, G., Simultaneous determination of super-oxide dismutase and catalase in biological materials by polarography. Anal. Biol. chem., 81, 157-166 (1977) https://doi.org/10.1016/0003-2697(77)90609-1
  20. Wei, H., Bowen, R., Cai, Q., Bames, S., and Wang, Y, Anti-oxidant and antipromotional effects of the soybean isoflavone genistein. Proc. Soc. Exp. Biol. Med., 208, 124-130 (1995). https://doi.org/10.3181/00379727-208-43844