Preventive Nutrition and Food Science

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Gamma-Aminobutyric Acid and/or Carnitine Supplementation Alters Lipid and Some Immune Related Nutrient Levels in Mice

  • Soh, Ju-Ryoun (Department of Food Science and Human Nutrition, Chonbuk National University) ;
  • Cha, Youn-Soo (Department of Food Science and Human Nutrition, Chonbuk National University)
  • Published : 2004.03.01
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Abstract

This study investigated the effects of carnitine and/or ${\gamma}$ -aminobutyric acid (GABA) supplementation on lipid profiles and some immune related nutrient in mice. Balb/c male mice were orally treated with either an AIN-76 diet (Con), a control diet plus carnitine (CS, 0.5 g/kg bw), a control diet plus GABA (GS, 0.5 g/kg bw) or a control diet plus carnitine plus GABA (CGS, 0.25 g/kg bw, respectively) for 6 weeks. There were no significant differences in feed consumption, energy intake, body weight gain or feed efficiency ratio among the groups during the experimental period. However, abdominal fat deposits were smaller in CS, GS and CGS groups compared with the Con group. Serum and liver triglycerides also were lower in CS, GS and CGS and serum total cholesterol was significantly lower in the CGS group compared with the Con group. Serum LDL cholesterol was lower in the CGS group and liver HDL cholesterol was significantly higher in the CS group compared with Con group. In serum, stearic acid and selecholeic acid were lower, but arachidic acid was higher in the CS group. Liver stearic acid was higher but oleic acid lower in CGS group compared with Con group. In carnitine supplemented groups, serum and liver nonesterified carnitine (NEC), acidsoluble acylcarnitine (ASAC), total carnitine (TCNE) concentrations were higher in only the CS group, not CGS group. Serum vitamin A and E concentrations were not different among the groups. These results may suggest that carnitine and/or GABA supplementation improves lipid profiles in mice, but did not affect the immune-related nutrients that we measured under the experimental conditions of this study.

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References

  1. Int J Sports Med v.11 L-carnitine supplementation in humans. The effects on physical performance Cerretelli PC;Marconi L https://doi.org/10.1055/s-2007-1024754
  2. Physiol Rev v.63 Carnitine-metabolism and functions Bremer J
  3. Ann Rev Biochem v.49 Regulation of hepatic fatty acid oxidation and ketone body production McGarry JD;Foster DW https://doi.org/10.1146/annurev.bi.49.070180.002143
  4. J Nutr v.116 Dose-dependent lipotropic effect of carnitine in chronic alcoholic rats Rhew TH;Sachan DS
  5. Biochem Biophys Res Comn v.203 Acetylcarnitine inhibits alcohol dehydrogenase Sachan DS;Cha YS https://doi.org/10.1006/bbrc.1994.2354
  6. Alcohol v.12 Acetyl carnitine-mediatd inhibition of ethanol oxidation in hepatocytes Cha YS;Sachan DS https://doi.org/10.1016/0741-8329(95)00007-E
  7. Phytochem v.29 Metabolism enzymology and possible roles of 4-aminobutyrate in higher plants Satyanarayan V;Nair PM https://doi.org/10.1016/0031-9422(90)85081-P
  8. Neurochem Res v.16 The structural and functional heterogeneity of glutamic acid decarboxylase Drlander MJ;Tobin AJ https://doi.org/10.1007/BF00966084
  9. Trends Neurosci v.17 Bringing the cleft at GABA synapses in the brain Mody I;Dekoninck Y;Otis TS;Soltesz I https://doi.org/10.1016/0166-2236(94)90155-4
  10. Receptor Pharmacology and Function GABA receptors Krogsgaard-Larsen P;Williams M(ed.);Glennon RA(ed.);Timmermans PMWM(ed.)
  11. J Biol Chem v.270 Brain L-glutamate decarboxylase Bao J;Cheung WY;Wu JY https://doi.org/10.1074/jbc.270.12.6464
  12. Center Line v.8 Researchers study alcohol's channels to the brain Morrow AL
  13. J Neurochem v.36 The uptake of carnitine by slices of rat cerebral cortex Huth PJ;Schmidt MJ;Hal PV;Fariello RG;Shug AL https://doi.org/10.1111/j.1471-4159.1981.tb01647.x
  14. Biochem Pharm v.30 Competitive inhibition by 3-amino propanesulfonic acid and γ-aminobutyric acid of carmitine transport in rat brain slices Fariello RG;Shug AL https://doi.org/10.1016/0006-2952(81)90049-6
  15. Biochem Buiphys Res Commun v.213 Effects of γ-aminobutric acid on the carnitine metabolism in neural cells Wawrencyk A;Nalecz KA;Nalecz MJ https://doi.org/10.1006/bbrc.1995.2143
  16. Nutraceut Food v.8 The effects of carnitine and/or gamma-aminobutyric acid (GABA) supplementation on the recovery of chronic ethanol administered rats Soh JR;Yamamoto TT;Cha YS https://doi.org/10.3746/jfn.2003.8.2.119
  17. International Immunopharmacology v.1 L-carnitine modifies the humoral immune response in mice after in vitro or in vivo treatment Athanassakis I;Mouratidou M;Sakka P;Evangeliou A;Spilioti M;Vassiliadis S https://doi.org/10.1016/S1567-5769(01)00105-9
  18. Food Sci Biotechnol v.12 Brown rice extracts with enhanced levels of GABA stimulate immune cells Oh SH;Oh CH
  19. Clin Chem v.29 A peroxidase-couple method for the colormetric determination of serum triglycerides McGowan MW;Artiss JD;Strandbergh DR;Zak B
  20. Clin Chem v.20 Enzymatic determination of total serum cholesterol Allain CC;Poon LS;Chan CS;Richman W;Fu PC
  21. Clin Chem v.28 HDL precipitation by dextran sulfate-MgCl₂method Warnick JB;Benderson J;Albers JJ
  22. Clin Chem v.18 Estimation of the concenration of low density lipoprotein cholesterol without use of the preparation ultracentrifuge Friedwald WT;Levy RL;Fredrickso DS
  23. J Food Sci v.50 Effects of oxygenabsorber and temperature on 3 polyunsatureated fatty acids of sardine oil during storage Suzuki H;Wada S;Hayakawa S;Tamura S https://doi.org/10.1111/j.1365-2621.1985.tb13401.x
  24. Anal Biochem v.72 A rapid and sensitive method fot the quantitation of microgram quatities of protein utilizing the principle of protein-dye binding Bradford M https://doi.org/10.1016/0003-2697(76)90527-3
  25. Clin Chim Acta v.37 Method for determination of carnitine in picomole range Cederblad G;Linstedt SA https://doi.org/10.1016/0009-8981(72)90453-6
  26. Am J Clin Nutr v.39 Ameliorating effects of carnitine and its precursors on alcohol-induced fatty liver Sachan DS;Rhew TH;Ruark RA
  27. Biometerics v.13 Multiple range test for corrrelated and heteroscedastic means Duncan DB
  28. J Clin Endocrinol Metab v.87 Leptin levels are associated with immune response in malnourished infants Palacio A;Lopez M;Pere-Bravo F;Monkeberg F;Schlesinger L https://doi.org/10.1210/jc.87.7.3040
  29. Proc Natl Acad Sci USA v.97 Leptin-deficient (ob/ob) mice are protected fro T cell-mediated hepatotoxicity. Role of tumor necrosis factor alpha and hepatotoxicity Faggioni R;Jones-Carson J;Reed DA;Dinarello CA;Feingold KR;Grunfeld C;Fantuzzi G https://doi.org/10.1073/pnas.040561297
  30. Clin Res v.30 Lipoprotein receptors:Genetic defense against atherosclerosis Goldstein JL;Brown MS
  31. J Am A v.264 Lipoproteins and atherogenesis Steinberg D;Witztum JL
  32. Eur J Appl Physiol Occup Physiol v.53 Effects of L-carnitine loading on the aerobic an anaerobic performance of endurance athletes Marconi C;Sassi G;Carpinelli A;Cerretelli P
  33. The American Physiological Society Carnitine supplementation and depletion:tissue carnitine and enzymes in fatty acid oxidation Negrao CE;Ji LL;Schauer JE;Nagle Fj;Lardy HA
  34. Eur J Appl Physiol v.65 Effects of carnitine loading on long-chain fatty acid oxidation, maximal exercise capacity, and nitrogen balance Heinonen OJ;Takala J;Molt K https://doi.org/10.1007/BF01466268
  35. J Nutr Biochem v.11 Increase in VO₂max and metabolic markers of fat oxidation by caffeine, carnitine, and choline supplementation in rats Sachan DS;Hongu N https://doi.org/10.1016/S0955-2863(00)00119-4
  36. J Appl Physiol v.63 Acetylcarnitine formation during intense muscular contraction in humans Harris RC;Foster CVL;Hultman E
  37. Nutr Res v.19 Effects of exercise training and/or high fat diet on lipid metabolism and carnitine concentration in rat Cha YS;Sohn HS;Daily Ⅲ JW;Oh SH https://doi.org/10.1016/S0271-5317(99)00054-8
  38. J Biochem Mol Biol v.34 Effects of regular endurance exercise of acute-exercise and rest on the levels of lipids, carnitine and carnitine palmitoyltransferase-Ⅰ in rats Cha YS;Kim HY;Soh JR;Oh SH
  39. J Nutr v.125 Choline supplementation alters carnitine homeostasis in humans and guinea pigs Daily Ⅲ JW;Sachan DS
  40. Vitam Horm v.59 Vitamin E and immunity Moriguchi S;Muraga M https://doi.org/10.1016/S0083-6729(00)59011-6
  41. Experimental Gernontology v.36 Caarnitine as a free radical scavenger in aging Juliet Arockia Rani P;Panneerselvam C https://doi.org/10.1016/S0531-5565(01)00116-4

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