Preventive Nutrition and Food Science

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Isolation of an Angiotensin Converting Enzyme Inhibitory Substance from Lycium chinense Miller

  • Lee, Sehee (Department of Food Science and Technology, College of Agriculture and Life Sciences, Chungnam National Uniyersity) ;
  • Song, Kyung-Bin (Department of Food Science and Technology, College of Agriculture and Life Sciences, Chungnam National Uniyersity)
  • Published : 2004.03.01
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Abstract

An angiotensin converting enzyme (ACE) inhibitory substance was isolated and purified from Lycium chinense Miller. A crude water extract of Lycium chinense Miller was prepared by adding it to water shaking at $25^{\circ}C$ for 1 hr, followed by centrifugation at 8000 ${\times}$ g for 30 min. The crude extract was then filtered using YM-3 and YM-1 membranes. An ACE inhibitor was isolated using consecutive chromatographic methods including: ion exchange chromatography, gel permeation chromatography, and FPLC. The inhibitor was identified to have a molecular mass of 862 daltons by mass spectrometry.

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