Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Antitumor and Antimutagenic Effect of the Proteinpolysaccharides from Polyporus umbellatus

저령다당체의 항종양 및 항돌연변이 효능

  • 이정화 (부산대학교 분자생물학과) ;
  • 신유진 (동서대학교 식품생명공학) ;
  • 조덕제 (동서대학교 식품생명공학) ;
  • 임희진 (동서대학교 식품생명공학) ;
  • 최원일 (대우약품 (주) ;
  • 이용규 (동서대학교 식품생명공학과)
  • Published : 2004.03.01
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Abstract

The proteinpolysaccharides (PPS) from Polyporus umbellatus (P. umbellatus) screlotium is composed by 78.2% of saccharide, 16.8% of protein, and 4.0% of ash. PPS from P. umbellatus showed antitumor activities against 180 solid tumor in ICR mice at the concentration of 20-160 mg/kg/day. PPS from P. umbellatus inhibited cell viability to 47.4% and 45.0% in leukemia cell lines, L-1210 and K-562 cells at 50-400 $\mu\textrm{g}$/mL concentration, respectively. But the hall mark of cell apoptosis, DNA fragmentation was not observed at those concentration. 2.5-10.0% of PPS from P. umbellatus inhibited mutagenecity evoked by 2-nitrofluorene and sodium azide in Salmonella typhimurium TA 98 and TA 100. From these results, it is suggested that the PPS of P. umbellatus has antitumor and antimutagenic effect, and its cytotoxic effect may not be ascribed to the apoptosis.

저령 버섯으로부터 추출된 다당체는 78.2%의 당류, 16.8%의 단백질 및 4%의 회분으로 구성되었다. 저령다당체는 20-160 mg/kg/day로 ,3주간 투여 시 ICR mouse에서 유발된 sarcoma-180에 대하여 항종양효능을 나타내었다. 또한 저령다당체를 50-400$\mu\textrm{g}$/mL의 농도로 투여시 백혈암세포인 L-1210와 K-562세포에서 대조군에 비하여 52.6%, 55.0%로 각각 성장하였다. 그러나 이들 세포에서 세포사멸의 생화학적인 증거가 되는 DNA 분절현상은 나타나지 아니하였다. 2.5-10.0%의 농도의 저령다당체는 Salmonella typhimurium TA 100과 TA 98에서 2-nitrofluorene과 sodium azide에 의해 유발된 돌연변이를56%까지 억제하였다 이 결과로 저령다당체는 항종양효능, 암세포성장 억제효능 및 항돌연변이 효능을 갖는 것으로 사료되며, 이 항종양 및 암세포 성장억제효능은 세포사멸(apoptosis)에 기인하지 않는 것으로 사료된다.

Keywords

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