Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Antimicrobial Activity of Clove Extract by Extraction Solvents

용매별 정향 추출물의 항균활성

  • 이옥환 (국립 한경대학교 식품생물공학과 식품생물산업연구소) ;
  • 정승현 (오뚜기중앙연구) ;
  • 손종연 (국립 한경대학교 식품생물공학과 식품생물산업연구소)
  • Published : 2004.03.01
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Abstract

This study was investigated the antimicrobial activity of clove extracts according to extraction solvents. The extracts were tested for their antimicrobial activity against several food spoilage microorganisms including Bacillus subtilis, Staphylococcus aureus, Escherichia coli, Salmonella typhimurium and Pseudomonas aeruginosa. The methanol extract showed stronger antimicrobial activities than water extract. However, petroleum ether extract did not show antimicrobial activity. The water extract of clove showed growth inhibition effect against Escherichia coli, Salmonella typhimurium and Staphylococcus aureus, whereas no effect against Bacillus subtilis. The methanol extract of clove extracts showed more sensitive antimicrobial activity in Gram (+) bacteria than in Gram (-) bacteria. The antimicrobial activities were increased with increasing concentration of the clove extract.

본 연구에서는 추출용매(물, 메탄올, 석유 에테르)에 따른 정향추출물의 항균성을 비교, 검토하고자 하였다. 물 추출물의 경우 E coli, S. aureus, S. typhimurium, P. aeruginosa에 대해서 항균효과를 나타내었으며 B. subtilis에서는 높은 농도에서도 항균력을 보이지 않았다. 메탄올 추출물의 경우 모든 균에서 항균성을 나타내었고, 특히 S. aureus에 대한 항균성이 매우 우수하였으며, 반면에 석유 에테르 추출물의 경우에서는 항균효과를 나타내지 않았다. 액체배지에서 정향의 생육저해 효과는 물 추출물의 경우 B. subtilis를 제외한 모든 균에서 항균력을 보였으며, 균종별로는 S. aureus보다 E. coli, S. typhimurium에서 더 강한 항균력 을 보였다. 메탄올 추출물의 생육저해 효과를 조사한 결과 모든 균에서 강한 항균력을 보였고, G(-)균보다 G(＋)균에 대한 생육저해 효과가 크게 나타났다. 반면 석유 에테르 추출물은 모든 균에 대해서 추출물의 농도에 관계없이 생육저해 효과를 보이지 않았다. 따라서 정향 추출물의 항균력은 메탄올>물>석유에테르 추출물 순이었다.

Keywords

References

  1. Park SK, Park JC. 1994. Antimicrobial activity of extracts and coumaric acid isolated from Artemisia princeps var. orientalis. Korean J Biotechnol Bioeng 9: 506-511.
  2. Kim YS, Kim MN, Kim JO, Lee JH. 1994. The effect of hot water-extract and flavor compounds of mugwort on microbial growth. J Korean Soc Food Nutr 23: 994-1000.
  3. Han JS, Shin DH. 1994. Antimicrobial effect of each solvent fraction of morus alba linne, sopora flavescens alton on Listeria monocytogenes. Korean J Food Sci Technol 26: 539-544.
  4. Park UY, Chang DS, Cho HR. 1992. Antimicrovial effect of lithospermi radix (Lithosperum erythrorhizon) extract. J Korean Soc Food Nutr 21: 97-100.
  5. Park UY, Chang DS, Cho HR. 1992. Screening of antimicrobial activity for medicinal herb extracts. J Korean Soc Food Nutr 21: 91-96.
  6. Lee HY, Kim CK, Sung TK, Mun TK, Lim CJ. 1992. Antibacterial activity of Ulmus pimila l. extract. Kor J Microbiol Biotechnol 20: 1-5.
  7. Hitokoto H, Morzumi S, Wauke T, Sakai S, Kurata H. 1980. Ingibitory efects of spices on growth and toxin production of toxigenic fungi. Appl Environ Microbiol 4: 818-826.
  8. Karapinar M, Aktug SE. 1987. Inhibition of food-borne pathogens by thymol, eugenol, menthol and anethole. Inter J Food Microbiol 4: 161-167. https://doi.org/10.1016/0168-1605(87)90023-7
  9. Kurita N, Miyaji M, Kurane R, Takahara Y. 1981. Antifungal activity of components of essential oils. Agric Biol Chem 45: 945-955. https://doi.org/10.1271/bbb1961.45.945
  10. Farrell KT. 1985. Spices, condiments, and seasonings. Van Noostrand Reinhold Co, New York. p 86-91.
  11. Park CS. 1998. Antibacterial acitivity of edible plant against pathogenic bacteria. Korean J Postharvest Sci Technol 5: 89-96.
  12. Ueda S, Yamashita H, Kuwabara Y. 1982. Inhibition of Clostridium botulinum and bacillus sp. by spices and flavouring compounds. Nippon Shokuhin Kogyo Gakkaishi 29: 389-397. https://doi.org/10.3136/nskkk1962.29.7_389
  13. Farag RS, Daw SY, Heweid FM, El-Baroty GSA. 1989. Antimicrobial activity of some egyptian spice essential oils. J Food Prot 52: 665-672.
  14. Farag RS, Daw ZY, Abo-Raya SH. 1989. Influence of some essential oils on Aspegillus parasiticus growth and production of aflatioxins in a synthetic medium. J Food Sci 54: 74-81. https://doi.org/10.1111/j.1365-2621.1989.tb08571.x
  15. Chung CK, Park OK, Yoo IJ, Park KM, Choi CU. 1990. Antimicrobial activity of essential oils of curry spices. Korean J Food Sci Technol 22: 716-719.
  16. Park CS, Choi MA. 1997. Effect of clove (Eugenia caryphyllata Thumb) on the survival of Listeria monocytogenes and Salmonella typhimurium during cold storage. Korean J Food Sci Technol 13: 602-608.
  17. Lee JI, Lee HS, Jun WJ, Yu KW, Shin DH, Hong BS, Cho HY, Yang HC. 2000. Physiological characteristics of anticoagulation fraction from Eugenia caryophyllata. J Korean Soc Food Nutr 29: 712-718.
  18. Park CS. 1998. Inhibition of Escherichia coli O157:H7 by clove. Korean J Soc Food Sci Nutr 14: 9-15.
  19. AOAC. 1990. Official methods of analysis. 15th ed. Association of official analytical chemists, Washington DC. USA.
  20. Kim MS, Lee DC, Hong JE, Chang KS, Cho HY, Kwon YK, Kim HY. 2000. Antimicrobial effects of ethanol extracts from Korean and Indonesian plants. Korean J Food Sci Technol 32: 949-958.
  21. Jung SH, Chung MS, Lee JS, Park KM. 2002. Antimutagenic effects of extracts of curry powder and its individual spice. Korean J Food Sci Ani Resour 22: 352-357.
  22. Baek JW, Chung SH, Moon GS. 2002. Antimicrobial activities of ethanol extracts from Korean bamboo culms and leaves. Korean J Food Sci Technol 6: 1073-1078.

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