Studies on the Selective Media for Bifidobacterium infantis Maeil-K9 Using Various Carbon Sources and Antibiotics

Bifidobacterium infantis Maeil-K9 균주의 당 발효 특성 및 항생제 내성을 이용한 선택배지 개발연구

  • 정병문 (매일유업(주) 중앙연구소) ;
  • 김응률 (매일유업(주) 중앙연구) ;
  • 정후길 (매일유업(주) 중앙연구) ;
  • 전호남 (매일유업(주) 중앙연구소)
  • Published : 2004.03.01

Abstract

To differentiate commercial bifidobacteia for Bifidobacterium lactis Bb-12, Bif. longum Bb-536 and Bif. infantis Maeil-K9, we studied the various carbon source, the nitrogen sources and antibiotics. Amygdalin and fructose were good candidates for carbon sources, and tryptone was suitable for nitrogen sources to design a new selective media for three commercial bifidobacteria. In the case of the amygdalin-containing medium as carbon sources, Bif. lactis Bb-12 and Bif. infantis Maeil-K9 showed good growth, and in fructose-containing medium, Bif. longum Bb-536 showed good growth. In antibiotics resistance study, the addition of 1 mg/L doxycyclin was very effective for differentiation of each bifidobacteria. Doxycyclin did not affect the growth of Bif. lactis Bb-12 and Bif. infantis Maeil-K9, but Bif. longum Bb-536 was completely inhibited by doxycyclin. Finally to confirm the selection capability of newly designed selective media, temperature-shocked bifidobacteria were cultured on them. As the results, fructose or doxycyclin containing medium showed for high growth for temperature-shocked bifidobacteria, but amygdalin containing medium showed low growth of temperature-shocked bifidobacteria.

현재 상업적으로 이용되고 있는 비피더스균인 Bifidobacterium lactis Bb-12, Bif. longum Bb-536 및 Bif. infantis Maeil-K9의 선택적 계수를 위한 선택배지 개발을 위하여 다양한 탄소원과 질소원 및 여러 항생제들에 대한 내성을 검토하였다. 탄소원의 경우 기본생육배지에 각각 15 g/L 수준으로 첨가하여 각균주의 생육성을 비교해 본 결과 amygdalin과 fructose가 가장 적합하였으며, 질소원의 경우 tryptone이 가장 적합한 것으로 나타났다. Amygdalin을 탄소원으로 이용한 경우 Bif. lactis Bb-12와 Bif. infantis Maeil-K9의 생육성이 우수하였으며, fructose를 이용한 경우 Bif. longum Bb-536의 생육성이 우수하였다. 항생제 내성 검토에 있어서는 doxycyclin을 1 mg/L 수준으로 첨가하는 것이 가장 적절하였으며, Bif. lactis Bb-12및 Bif. infantis Maeil-K9의 생육에는 전혀 영향을 주지 않았으나, Bif. longum Bb-536의 생육은 완전히 억제시켰다. 그리고 저 활성 균주에 대한 각 선택 배지의 검출능을 알아보기 위하여 각균주를 -$70^{\circ}C$, $4^{\circ}C$, $40^{\circ}C$에 2주간 보존 후 생균수를 측정한 결과 amygdalin을 탄소원으로 이용한 배지에서는 $40^{\circ}C$ 보존균주의 검출능이 낮았으나, fructose를 탄소원으로 이용한 배지와 doxycyclin을 첨가한 배지의 경우 그 선택성이 매우 우수하였다.

Keywords

References

  1. Bifidobacteria Microflora v.12 Isolation and identification of Bifidobacterium Sp. in commercial yoghurts sold in Europe Hirokazu,I.H.;Masuda,T.Fujisawa;H.Suzuki;T.Mitsuoka https://doi.org/10.12938/bifidus1982.12.1_39
  2. Lett. Appl. Microbiol v.9 The presence of plasmids in Bifidobacterium breve Iwata,M.;T.Morishita
  3. J. Korean Dairy Technol. Sci. v.19 Adherence of probiotics to gastrointestinal track and their promoting factors Jung,H.K.;E.R.Kim;S.L.Juhn
  4. Korean J. Food Sci. Ani. Resour. v.19 Selective detection and enumeration of Bifidobacterium longum MK-G7 by using sugar alcohol and antibiotics Kim,E.R.;H.K.Jung;S.L.Juhn
  5. Bioscience Microflora v.21 Effects pf bifidobacteria-deriverd crude peptidoglycan on protection against E. coli O157:H7 and rotavirus infection Kim.E.R;H.K.Jung;H.N.Chun;S.L.Juhn;B.S.Park;D.H.Yu;J.H.Ju https://doi.org/10.12938/bifidus1996.21.251
  6. Korean J. Food Sci. Ani. Resour. v.21 Factors affecting the adherence of bifidobacteria to Caco-2 cell Kim;E.R.;Jung,H.K;Juhn,S.L.;Yu,J.H.
  7. J. Microbiol. Biotechnol. v.11 Characterization of plasmid from Bifidobacterium sp Lee,J.H.;M.S.Park;K.H.Lee;G.E.Ji
  8. Kor. J. Appl. Microbiol. Biotechnol. v.22 Selective media containing antibiotics for counting bifidobacteria Lee,J.J.;M.S.Shin;S.H.Na;H.S.;Bae;Y.J.Baek
  9. J. Korean. Soc. Food. Sci. Nutr. v.25 Selective media for isolation and enumeration of lactic acid bacteria from Kimchi Lee,M.K.;W.S.Park;K.H.kang
  10. Korean J. Food Sci. Technol. v.31 Selection and enumeration of bifidobacteria in fermented foods with various lactic acid bactera Lee,S.K.;D.K.Park;H.I.Oh;J.H.Park
  11. J. Microbiol. Sep. Effect of lactic acid bacteria on intestinal microbial enzyme activity and composition in rats treated with azoxymethane Lee,S.M.;W.K.Lee
  12. Bifidobacteria Microflora v.1 Recent trends inresearch on intestinal flora Mitsuoka,T. https://doi.org/10.12938/bifidus1982.1.1_3
  13. Kor. J. Appl. Microbiol. Biotechnol. v.23 Studies on the selective screening method of bifidobacteria used in yoghurt Park,H.K;T.R.Heo
  14. Bifidobacteria and Their Role Rasic,J.L.;J.A.Kurmann
  15. A Review. Vet. human Toxicol. v.35 Cyanide Poisioning in Animals and Humans Salkowski;Alicia A;David G. Penney
  16. J. Gen. Microbiol v.128 Plasmids in the genus bifidobacteria Seobarti,B.;V.Scardovi;D.J.Leblanc
  17. Mocrobiogia v.9 Relative structure in the piasmid profiles of Bifidobacterium longum Sgobarti,B.;V.Scardovi;D.J.Leblanc
  18. Kor. J. Appl. Microbiol. Biotechnol v.22 Selective media for the isolation and counting of bifidobacteria in dairy products Shin,M.S.;J.J.Lee;I.Y.Shu;S.H.Na;Y.J.Baek
  19. Jpn. J. Bacteriol v.33 Enumeration of bifidobacteria in dairy product Teraguchi,S;M.Uehara;K.Ogasa;T.Mitsuoka https://doi.org/10.3412/jsb.33.753