Biotechnology and Bioprocess Engineering:BBE

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
권호 표지

Microcontact Printing of Biotin for Selective Immobilization of Streptavidin-fused Proteins and SPR Analysis

  • Lee, Sang-Yup (Department of Chemical and Biomolecular Engineering, Bioprocess Engineering Research Center, Center for Ultramicrochemical Process Systems, Korea Advanced Institute of Science and Technology, Department of Chemical and Biomolecular Engineering, Bioprocess Engineering Research Center, Department of BioSystems, Bioinformatics Research Center, Korea Advanced Institute of Science and Technology) ;
  • Park, Jong-Pil (Department of Chemical and Biomolecular Engineering, Bioprocess Engineering Research Center, Center for Ultramicrochemical Process Systems, Korea Advanced Institute of Science and Technology) ;
  • Lee, Seok-Jae (Department of Chemical and Biomolecular Engineering, Bioprocess Engineering Research Center, Center for Ultramicrochemical Process Systems, Korea Advanced Institute of Science and Technology) ;
  • Park, Tae-Jung (Department of Chemical and Biomolecular Engineering, Bioprocess Engineering Research Center, Center for Ultramicrochemical Process Systems, Korea Advanced Institute of Science and Technology) ;
  • Lee, Kyung-Bok (Department of Chemistry, Korea Advanced Institute of Science and Technology) ;
  • Park, Insung S. (Department of Chemistry, Korea Advanced Institute of Science and Technology) ;
  • Kim, Min-Gon (BioNanotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Chung, Bong-Hyun (BioNanotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology)
  • Published : 2004.03.01
Download PDF
⟨ Previous Next ⟩

Abstract

In this study, a simple procedure is described for patterning biotin on a glass substrate and then selectively immobilizing proteins of interest onto the biotin-patterned surface. Microcontact printing (CP) was used to generate the micropattern of biotin and to demonstrate the selective immobilization of proteins by using enhanced green fluorescent protein (EGFP) as a model protein, of which the C-terminus was fused to a core streptavidin (cSA) gene of Streptomyces avidinii. Confocal fluorescence microscopy was used to visualize the pattern of the immobilized protein (EGFP-cSA), and surface plasmon resonance was used to characterize biological activity of the immobilized EGFP-cSA. The results suggest that this strategy, which consists of a combination of $\mu$CP and cSA-fused proteins. is an effective way for fabricating biologically active substrates that are suitable for a wide variety of applications. one such being the use in protein-protein assays.

Keywords

References

  1. Biomaterials v.19 Protein patterning Blawas,A.S.;W.M.Reichert https://doi.org/10.1016/S0142-9612(97)00218-4
  2. Trends Biotechnol. v.13 Patterning self-assembled monolayers using microcontact printing: A new technology for biosensors Mrksich,M.;G.M.Whitesides https://doi.org/10.1016/S0167-7799(00)88950-7
  3. Angew. Chem. Int. Ed. v.42 Recent developments in protein microarray technology Wilson,D.S.;S.Nock https://doi.org/10.1002/anie.200390150
  4. Curr. Opin. Chem. Biol. v.7 Protein chip technology Zhu,H.;M.Snyder https://doi.org/10.1016/S1367-5931(02)00005-4
  5. Curr. Opin. Chem. Biol. v.6 Functional protein microarrays Wilson,D.S.;S.Nock https://doi.org/10.1016/S1367-5931(01)00281-2
  6. Proc. Natl. Acad. Sci. USA v.93 Patterning of functional antibodies and other proteins by photolithography of silane monolayers Mooney,J.F.;A.J.Hunt;J.R.Mclntosh;C.A.Librerko;D.M.Walba;C.T.Rogers https://doi.org/10.1073/pnas.93.22.12287
  7. Nanotechnology v.7 Creation of biomolecule arrays by electrostatic immobilization on electron-beam-irradiated polystyrene thin films Wybourne,M.N.;M.Yan;J.K.W.Keana;J.C.Wu https://doi.org/10.1088/0957-4484/7/3/021
  8. Bioconjugate Chem. v.7 Protein patterning with a photoactivatable derivative of biotin Hengsakul,M.;A.E.G.Cass https://doi.org/10.1021/bc960007z
  9. Langmuir v.14 Micropatterning of biomolecules on polymer substrates Schwarz,A.;J.S.Rossier;E.Roulet;N.Mermod;M.A.Roberts;H.H.Girault https://doi.org/10.1021/la980359p
  10. Biomaterials v.19 Adhesion of mammalian cells to polymer surfaces: from physical chemistry of surfaces to selective adhesion on defined patterns Dewez,J.L.;J.B.Lhoest;E.Detrait;V.Berger;C.C.Dupont-Gillain;L.M.Vincent;Y.J.Schneider;P.Bertrand;P.G.Rouxhet https://doi.org/10.1016/S0142-9612(98)00055-6
  11. Bioconjugate Chem. v.7 A general method for the spatially defined immobilization of biomolecules on glass surfaces using "caged" biotin Michael,C.P.;C.Y.Huang https://doi.org/10.1021/bc960013v
  12. Nucleic Acids Res. v.28 Oligonucleotide immobilization on micropatterned streptavidin surfaces Sabanayagam,C.R.;C.L.Smith;C.R.Cantor https://doi.org/10.1093/nar/28.8.e33
  13. Nucleic Acids Res. v.25 Molecular cloning and nucleotide sequence of the streptavidin gene Argarana,C.E.;I.D.Kuntz;S.Birken;R.Axel;C.R.Cantor
  14. Molecular Cloning: A Laboratory Manual(3rd ed.) Sambrook,J.;D.Russell
  15. J. Am. Chem. Soc. v.111 Formation of monolayer films by the spontaneous assembly of organic thiols from solution onto gold Bain,C.D.;E.B.Troughton;Y.T.Tao;J.Evall;G.M.Whitesides;R.G.Nuzzo https://doi.org/10.1021/ja00183a049
  16. Langmuir v.17 Microstamping on an activated polymer surface: Patterning biotin and streptavidin onto common polymeric biomaterials Hyun,J.;Y.Zhu;A.Liebmann-Vinson;T.P.Beebe;A.Chilkoti https://doi.org/10.1021/la010695x
  17. Bull. Korean Chem. Soc. v.24 Pattern generation of cells on polymeric surfaces using surface functionalization and microcontact printing Lee,K.B.;Y.Kim;I.S.Choi https://doi.org/10.5012/bkcs.2003.24.2.161
  18. Korean J. Chem. Eng. v.20 Patterning Si by using surface functionalization and microcontact printing with a polymeric ink Lee,K.B.;D.J.Kim;K.R.Yoon;Y.Kim;I.S.Choi https://doi.org/10.1007/BF02697305
  19. J. Pharm. Sci. v.92 Surface modification of poly(glycolic acid) (PGA) for biomedical applications Lee,K.B.;K.R.Yoon;S.I.Woo;I.S.Choi https://doi.org/10.1002/jps.10556
  20. Langmuir v.20 Pattern generation of biological ligands on a biodegradable poly(glycolic acid) (PGA) film Lee,K.B.;D.J.Kim;Z.W.Lee;S.I.Woo;I.S.Choi https://doi.org/10.1021/la036209i