Antimutagenic and Cytotoxic Effects of Allium victorialis Extracts

산마늘 추출물의 항돌연변이원성 및 세포독성 효과

  • 함승시 (강원대학교 바이오산업공학부) ;
  • 최승필 (강원대학교 바이오산업공학) ;
  • 최형택 ((주)신원에프아) ;
  • 이득식 (동해대학교 외식산업학과)
  • Published : 2004.06.01

Abstract

This study was carried out to determine the antimutagenic and anticancer effects of Allium victoriolis using Ames test and cytotoxicity. Allium victorialis extracted with ethanol and then further fractionated to chloroform, ethyl acetate and water. The inhibition rate of ethanol extract (200 $\mu\textrm{g}$/plate) of Allium victorialis in the Salmonella typhimurium TA100 strain showed 88.2% against the mutagenesis induced by N-methyl-N'-nitro-N-nitrosoguanidine(MNNG). The suppression ratio against 4-nitroquinoline-1-oxide(4NQO) in the Salmonella typhimurium TA98 and TA100 strains showed 76.4% and 83.0%, respectively. The cytotoxicity effects of Allium victorialis extract against the cell lines with human lung carcinoma (A549), human breast adenocarcinoma (MCF-7) and human gastric cacinoma (KATOIII) were inhibited with the increase of extract concentration. The treatment of 0.5 $\mu\textrm{g}$/plate Allium victorialis of ethanol extract showed strong cytotoxicities of 74.2%, 71.3% and 67.4% against A549, MCF-7 and KATOIII, respectively.

산마늘 에탄올 추출물과 각종 유기용매 분획물에 대하여 항돌연변이원성과 세포독성을 검토하였다. 시료자체는 돌연변이원성이 없는 것으로 나타났다. 직접 변이원인 MNNG에 대한 항돌연변이 효과에서 S. typhimurium TA100 균주에 대해 산마늘 에탄올 추출물(200$\mu\textrm{g}$/plate)에서 88.2%의 억제효과를 나타내었다. 동일 시료 농도에서 4NQO에 대해서는 S. typhimurim TA98과 TA100 두 균주 모두에서 각각 76.4%와 83.0%의 비교적 높은 억제효과를 나타내었다. 암세포 성장 억제 효과를 검토한 실험에서는 에탄올 추출물이 다른 분획물보다 높은 억제활성을 나타내었다. 시료농도의 증가와 함께 억제활성도 증가하는 경향을 나타내었으며 시료농도 50 $\mu\textrm{g}$/plate에서 A549가 74.2%, MCF-7이 71.3% 그리고 KATOIII에 대하여 67.4%의 암세포 성장억제효과를 나타내었다.

Keywords

References

  1. 이창복 (1982) 대한식물도감. 향문사, p.203
  2. 문관심 (1988) 약초의 성분과 이용. 평양, 과학백과사전출판사, p.671
  3. Nishimura, H.K., Fujiwara, J., Mizutani, J. and Obata, Y. (1971) Volatile flavor components of caucas. J. Agr. Food. Chem., 19, 992-994 https://doi.org/10.1021/jf60177a042
  4. Lim, S.C., Park, H.J., Yun, S.Y., Lee M.S., Kim, W.B. and Jung, W.T. (1996) Structures of flavonoids and furostanol glycosides isolated from the bulbs of Allium victorialis L. J. Kor. Soc. Hort. Sci., 37, 675-679
  5. Park, H.J., Kim, W.B. Yoo, K.O. and Jung, W.T. (1998) Chemical analysis on biological active substances among habitats of Allium victorialis for a high income crop. Kor. J. Plant. Res., 11, 51-60
  6. Choi, S.T., Lee, J.T. and Park, W.C. (1993) Growth environment and nutritional evaluation of native Allium victorialis var. Platyphyllum in Ulrung island. J. Kor. Agric. Chern. Soc., 36, 502-509
  7. Lee, S.S., Moon, S.H., Lee, H.J., Choi, D.H. and Cho, M.H. (2004) Cholesterol inhibitor activities of kaempferol and quercetin isolated form Allium victorialis var. platypbyllum. Mokchae Konghak., 32, 17-27
  8. Kim, T.G., Kim, S.H., Kang, S.Y., Jung, K.K., Choi, D.H., Park, Y.B., Ryu, J.H. and Han, H.M. (2000) Antiatherogenic effect of the extract of Allium victorialis on the experimental atherosclerosis in the rabbit and transgenic mouse. Kor. J. Phannacon., 31, 149-156
  9. Choi, J.W., Lee, K.T, Kim, W.B., Park, K.G., Jung, H.J. and Park, H.J. (2003) Pharmcological effects of the Allium victorialis var. Platyphyllum extract on the rats induced by streptozotocin, poloxamer-407, CCI4 and Galactosaminen. Kor. J. Phannacon., 34, 149-156
  10. Yahagi, T., Nagao, M., Seino, Y., Matsushima, T., Sugimura, T. and Okada, M. (1997) Mutagenicities of N-nitrosomines on Salmonella. Mutation Res., 48, 121-130
  11. Scudiero, D.A., Shoemaker, R.H., Paul, K.D., Monks, A., Tiemey, S., Nofziger, T.H., Currens, M.J., Seniff, D. and Boyd, M.R. (1988) Evaluation of a soluble tetrazolium/formazan assay for cell growth and drug sensitivity in culture using human and other tumor cell lines. Cancer Res., 48, 4827-4836
  12. Martin, A. and Martin, C. (1997) Comparision of 5 microplate colorinIetric assay for in vitro cytotoxicidy testing and cell proliferation assays. Cytotechnology, 11, 49-54 https://doi.org/10.1007/BF00749057
  13. Ham, S.S., Han, H.S., Choi, K.P. and Oh, D.H. (1997) Inhibitory effects of synurus deltoides extracts on the mutagenesis induced by various mutagens. J. Kor. Soc. Food Nutr., 26, 528-533
  14. Han, K.S., Ham, S.S., Jeong, E.H. and Lee, H.K. (1992) Antimutagenic effects of the edible mountain herb juices against Trp-P-1 and 2-AF. Kor. J. Food Hygiene, 7, 161-168
  15. Han, K.S., Jeong, E.H., Ham, S.S., Shim, T.H., Lee, T.S. and Lee, H.K. (1993) Antimutagenicity of small water dropwort juice on the microbial mutagenicity induced by 2-antinofluorene. Kor. J. Food Hygiene, 8, 225-230
  16. Ham, S.S., Chung, C.K, Lee, J.H., Choi, K.P., Jung, S.W. and Kim, EJ. (1998) Antimutagenicuty and cytotoxicity of Artemisia iwayomogi kitamura extracts. J. Kor. Soc. Food Nutr., 27, 157-162
  17. Im, S.Y. (1994) Antimutation and anti-tumor effect of linoleic acid. M.S. Thesis, Pusan national University
  18. 지은정, 정갑용, 강미경 (1999) 한국인이 식품으로 사용하는 생리적 농도에서의 마늘의 항암효과. 전북의대논문집, 23,1-10