Food Science and Preservation (한국식품저장유통학회지)

The Korean Society of Food Preservation (한국식품저장유통학회)
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Effect of Short-term High $CO_2$ on Growth of Botrytis cinerea

고농도 이산화탄소의 단기 처리가 Botrytis cinerea 생장에 미치는 영향

  • Published : 2004.06.01
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Abstract

This study was conducted to evaluate the inhibition efficacy of high CO$_2$ atmosphere (35, 60, and 100% ${\times}$ 24, 48, and 72 h) on growth of Botrytis cinerea in vitro in order to offer sterilizing method of horticultural crops including peach fruits. Botrytis cinerea was isolated from a naturally infected peach fruits. Growth of the fungus at 25$^{\circ}C$ declined with increased CO$_2$ concentration and treatment duration. Especially, 100% CO$_2$ provided completely inhibition effect of growth of the fungus for 72 h. After removal of high CO$_2$ condition, however, the fungus showed normal growth speed. The growth of fungus at low temperature was completely inhibited temporarily by short-term 100% CO$_2$ treatment, but resumed right after transferring to normal atmosphere at 25$^{\circ}C$.

이산화탄소 처리에 따른 곰팡이의 생장억제 정도를 구명하기 위해 부패된 '황도' 복숭아로부터 분리된 Botrytis cinerea를 대상으로 본 연구를 수행하였다. 35, 60 또는 100%의 이산화탄소를 각각 24, 48 또는 72시간 동안 처리하여 곰팡이의 생장정도를 측정하였다. 상온조건에서 배양할 경우 고농도 이산화탄소 처리를 통해 생장속도를 억제할 수 있었으며 농도 및 처리기간이 증가할수록 효과가 증가하였다. 특히 100% 농도의 경우 처리기간 동안 완전한 생장 억제효과를 나타내었다. 그러나 고농도 이산화탄소 처리가 해제된 이후에는 정상적인 생장이 일어났다. 저온에 의해 곰팡이 생장이 효과적으로 억제되었으며 저온에서는 35%와 60％의 이산화탄소를 처리할 경우 추가적인 억제효과는 없었으며, 100% 이산화탄소를 72시간 동안 처리함으로써 곰팡이 생장을 효과적으로 억제할 수 있었다. 저온 및 이산화탄소 처리에 의해 생장이 억제된 Botrytis cinerea를 상온으로 옮겨 배양할 경우 급격한 생장을 보인 바 저온 및 이산화탄소 처리에 의해 Botrytis cinerea가 사멸되는 것이 아니라 일정 기간 생장이 억제되는 것으로 판단되었다.

Keywords

References

  1. Nelson, K.E., (1979) Harvesting and handling California table grapes for market. University of California Bulletin, no. 1913
  2. Winkler, A.J., Cook, J.A., Kliewer, W.M. and Lider, L.A. (1974) General Vjticulture, second ed. University of California Press, p.710
  3. Lisker, N., Keren-Shacham, Z., Sarig, P., Zutkhi, Y. and Ben-Arie, R. (1996) The biology and pathology of the fimgus Rhizopus stolonifer, cause of black mould disease of table grapes in Israel. Plant Pathol., 45, 1099-1109 https://doi.org/10.1046/j.1365-3059.1996.d01-10.x
  4. Taylor, S. (1993) Why sulfite alternatives? Food Technol., 47, 14
  5. Berry, G. and Aked, J (1997) Controlled atmosphere alternatives to the post-harvest use of sulphur dioxide to inhibit the development of Botrytis cinerea in table grapes. Proceedings of the Seven International Controlled Atmosphere Research Conference, vol. 3, UC Davis Postharv. Hortic. Series, 17, 160-164
  6. Phillips, D.J. and Austin, R.K. (1982) Changes in peaches after hot-water treatment. Plant Dis., 66, 487-488 https://doi.org/10.1094/PD-66-487
  7. Sommer, N.F., Mitchell, E.G., Fortlage, R,J., Mayer, G. and Guillou, R. (1967) Heat treatment for brown not control in peaches and nectarines. Blue Anchor, 45, 9-14
  8. Ahumada, M.H., Mitcham, E.J. and Moore, D.G. (1996) Postharvest quality of Thompson Seedless grapes after insecticidal controlled-atmosphere treatments. HortScience, 31, 833-836
  9. Crisosto, C.H., Gamer, D. and Crisosto, G. (2002) Carbon dioxide-enriched atmospheres during cold storage limit losses from Botrytis but accelerate rachis browning of 'Redglobe' table grapes. Postharvet Biol. Technol., 26, 181-189 https://doi.org/10.1016/S0925-5214(02)00013-3
  10. Tian, S., Fan, Q., Xu, Y., Wang, Y. and Jiang, A. (2001) Evaluation of the use of high CO2 concentrations and cold storage to control Monilinia fructicola on sweet cherries. Postharvest BioI. Technol., 22, 53-60 https://doi.org/10.1016/S0925-5214(00)00177-0
  11. De Vries-Paterson, R.M., Jones, A.L. and Camerson, A.C. (1991) Fungistatic effects of carbon dioxide in a package environment on the decay of Michigan sweet cherries by Monilinia frueticola. Plant Dis., 75, 943-946 https://doi.org/10.1094/PD-75-0943
  12. Tian, S. and Bertolini, P. (1995) Effects of low temperature on mycelial growth and spore germination of Botrytis allii in culture and on its pathologenecity to stored garlic bulbs. Plant Pathol., 44, 1008-1015 https://doi.org/10.1111/j.1365-3059.1995.tb02659.x
  13. Bertolini, P. and Tian, S.P. (1996) Low temperature biology and pathogenicity of Penicillium hirsutum on garlic in storage. Postharvest Biol. Technol., 7, 83-89 https://doi.org/10.1016/0925-5214(95)00025-9