Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Purification and Characterization of $\beta$-Xylosidase from Trichoderma sp. SY

  • Kim, Bong-Gyu (Department of Forest and Environmental Science, Bio/Molecular Informatics Center, Konkuk University) ;
  • Jung, Bo-Ra (Department of Advanced Fusion Technology, Bio/Molecular Informatics Center, Konkuk University) ;
  • Jung, Jae-Gyu (Department of Forest and Environmental Science, Bio/Molecular Informatics Center, Konkuk University) ;
  • Hur, Hor-Gil (Department of Environmental Science and Engineering, Gwangju Institute of Science and Technology) ;
  • Ahn, Joong-Hoon (Department of Forest and Environmental Science, Department of Advanced Fusion Technology, Bio/Molecular Informatics Center, Konkuk University)
  • Published : 2004.06.01
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Abstract

A $\beta$-xylosidase was purified from the culture of Trichoderma sp. SY. The ten-day-old culture filtrate was concentrated, followed by ion-exchange chromatography and gel filtration chromatography. As a result, $\beta$-xylosidase was finally purified about 53-fold and appeared as a single band by SDS-PAGE. The optimum pH and temperature were 5.0 and $55^{\circ}C$, respectively, and the molecular weight about 80 kDa. The purified $\beta$-xylosidase was found to be inhibited by various metal ions but not inhibited by xylose.

Keywords

References

  1. Curr. Opin. Biotechnol. v.11 Metabolic engineering applications to renewable resource utilization Aristidou,A.;M.Penttila https://doi.org/10.1016/S0958-1669(00)00085-9
  2. Bioresoruce Technology v.90 Purification and properties of an extracelluar β-xylosidase from a newly isolated Fusarium proliferatum Badal,C.S. https://doi.org/10.1016/S0960-8524(03)00098-1
  3. Trends Biotechnol. v.3 Microbial xylandolytic systems Biely,P. https://doi.org/10.1016/0167-7799(85)90004-6
  4. Fungal Genet. Biol. v.25 Carbon catabolite repression of gene expression and condiation in Neuospora crassa Ebblole,D.J. https://doi.org/10.1006/fgbi.1998.1088
  5. Biochem. J. v.321 The β-D-xylosidase of Trichoderma reesei is a multifunctional β-D-xylan xylohydrolase Hermann,M.C.;M.Vrsanska;M.Jurickova;J.Hirsch;P.Beily;C.P.Kubicek https://doi.org/10.1042/bj3210375
  6. J. Microbiol. Biotechnol. v.12 Cellulase-free thermostable alkaline xylanase from thermophilic and alkalophilic Bacillus sp. JB-99 Johnvesly,B.;S.Virupakshi;G.N.Patil;Ramalingam;G.R.Naik
  7. J. Microbiol. Biotechnol. v.13 Isolation and characteristics of Trichoderma harzianum FJ1 producing cellulases and sylanase Kim,K.C.;S.S.Yoo;Y.A.Oh;S.J.Kim
  8. FEMS Microbiol. Rev. v.23 Molecular and biotechnological,aspects of xylanases Kulkarni,N.;A.Shendye;M.Rao https://doi.org/10.1111/j.1574-6976.1999.tb00407.x
  9. FEMS Microbiol. Lett. v.135 Purification and regulation of the synthesis of a β-xylosidases from Aspergillus nidulans Matsuo,M.;A.Fujie;M.Win;T.Yasui
  10. Agric. Biol. Chem. v.51 Four types of β-xylosidases from Penicilium wortmanni IFO 7237 Matsuo,M.;A.Fujie;M.Win;T.Yasui https://doi.org/10.1271/bbb1961.51.2367
  11. J. Microbiol. Biotechnol. v.12 Purification,characterization,and cDNA cloning of xylanase from fungus Trichoderma strain SY. Min,S.Y.;B.G.Kim;C.Lee;H.G.Hur;J.H.Ahn
  12. J. Bacteriol. v.183 The wide-domain carbon catabolite repressor CreA indirectly controls expression of the Aspergillus nidulans xlnB gene,encoding the acidic endo β-(1,4)-xylanase X24 Orejas,M.;A.P.MacCabe;J.A.Prez Gonzlez;S.Kumar;D.Ramn https://doi.org/10.1128/JB.183.5.1517-1523.2001
  13. Carbohydr. Res. v.297 Purification and characterization of extracellular β-xylosidase and α-arabinosidase from the plant pathogenic fungus Cochliobolus carbonum Ransom,R.F.;J.D.Walton https://doi.org/10.1016/S0008-6215(96)00281-9
  14. J. Ferment. Bioeng. v.79 Purification and some properties of Aspergillus pulverulentus β-xylosidase with transxylosylation capacity Sulistyo,J.;Y.Kamiyama;T.Yasui https://doi.org/10.1016/0922-338X(95)92737-W
  15. FEMS Microbiol. Rev. v.104 Molecular biology of xylan degration Thompson,J.A. https://doi.org/10.1111/j.1574-6968.1993.tb05864.x
  16. Microbiology v.145 A unique eukaryotic β-xylosidase gene from the phytophathogenic fungas Cochilobolus carbonum Wegener,S.;R.F.Ransom;J.D.Walton https://doi.org/10.1099/13500872-145-5-1089