Korean Journal of Microbiology (미생물학회지)

The Microbiological Society of Korea (한국미생물학회)
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Functional Expression of the Neurospora crassa coq-4 Gene in Saccharomyces cerevisiae.

Saccharomyces cerevisiae에서 Neurospora crassa coq-4 유전자의 기능적 발현

  • 김은정 (고신대학교 생명과학부) ;
  • 최상기 (순천대학교 생명과학) ;
  • 천재우 (순천향대학교 생명과학부 유전공학) ;
  • 오계헌 (순천향대학교 생명과학부 유전공학) ;
  • 이병욱 (고신대학교 생명과학부)
  • Published : 2004.06.01
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Abstract

Coenzyme Q is a quinone derivative that acts as a lipid electron carrier in the respiratory chain located at mito-chondrial inner membrane in eucaryotes or plasma membrane in procaryotes and also functions as antioxidant. A putative Neurospora crassa coq-4 gene was cloned and functionally expressed in Saccharomyces cerevisiae coq4 mutant. Complemented S. cerevisaie mutant strain was able to produce coenzyme $Q_{6}$ and showed a normal growth rate. They also showed less sensitivities to polyunsaturated fatty acids such as linoleic acid or linolenic acid. The predicted sequence of N. crassa COQ4 is consisted of 347 amino acids with a molecular mass of 39.7 kDa and showed 35% identity and 52% similarity with that of S. cerevisiae.

Coenzyme Q는 지용성 퀴논 유도체로서 미토콘드리아의 내막과 원핵생명체의 세포막에 위치하는 전자전달계에서 전자 운반체로 이용되며 또한 항산화제의 기능도 갖는다. Coenzyme Q의 생합성에 관여하는 Saccharomyces cerevisiae의 coq4 유전자에 유사성을 나타내는 Neurospora crassa coq-4유전자를 클로닝하여 S. cerevisiae coq4 돌연변이체에서 기능적으로 발현하였다. 상보된 S. cerevisiae 균주들은 coenzyme $Q_{6}$의 생산능력을 회복하였으며 정상적인 성장률을 보였다. 또한 linoleic acid 및 linolenic acid와 같은 불포화지방산에 대한 낮은 감수성을 보였다. N. crassa의 COQ4 단백질은 39.7 kDa의 분자량을 갖는 347개의 아미노산으로 구성되어 있는 것으로 예상되며, S. cerevisiae의 Coq4p와 35%의 일치도 및 52%의 유사도를 보인다.

Keywords

References

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