Preventive Nutrition and Food Science

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Isolation of a Tryptophan-Overproducing Strain Generated by EMS Mutagenesis of Candida rugosa

  • Kim, Sun-Hye (Department of Food Science and Technology, Chungnam National University) ;
  • Ha, Yu-Mi (Department of Food Science and Technology, Chungnam National University) ;
  • Youm, Hyoung-Joon (Department of Food Science and Technology, Chungnam National University) ;
  • An, Gil-Hwan (Department of Food Science and Technology, Chungnam National University) ;
  • Lee, Bong-Duk (Department of Animal Science, Chungnam National University) ;
  • Won, Mi-Sun (Genome Research Center, Korea &search Institute of Bioscience and Biotechnolog) ;
  • Song, Kyung-Bin (Department of Food Science and Technology, Chungnam National University)
  • Published : 2004.06.01
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Abstract

To isolate a mutant strain which overproduces tryptophan, mutants of Candida rugosa were screened after EMS (ethyl methane sulfonate) mutagenesis. Fluorotryptophan, a tryptophan analogue, was used for selection of a tryptophan-overproducing mutant after mutagenesis. Among 50 mutants, several candidates were selected based on intracellular tryptophan content. Amino acid analysis results showed that C3 was the best strain because it had the highest amount of tryptophan among the mutants.

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References

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