Biotechnology and Bioprocess Engineering:BBE

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
권호 표지

Impeller Types and Feeding Modes Influence the Morphology and Protein Expression in the Submerged Culture of Aspergillus oryzae

  • Heo, Joo-Hyung (Metabolic Engineering Research Laboratory, Korea Research Institute of Bioscience and Biotechnology, BioHoldings Inc., BVC #201, Korea Research Institute of Bioscience and Biotechnology) ;
  • Vladimir Ananin (Metabolic Engineering Research Laboratory, Korea Research Institute of Bioscience and Biotechnology) ;
  • Park, Jeong-Seok (Metabolic Engineering Research Laboratory, Korea Research Institute of Bioscience and Biotechnology) ;
  • Lee, Chung-Ryul (Metabolic Engineering Research Laboratory, Korea Research Institute of Bioscience and Biotechnology) ;
  • Moon, Jun-Ok (BioHoldings Inc., BVC #201, Korea Research Institute of Bioscience and Biotechnology) ;
  • Ohsuk Kwon (Metabolic Engineering Research Laboratory, Korea Research Institute of Bioscience and Biotechnology) ;
  • Kang, Hyun-Ah (Metabolic Engineering Research Laboratory, Korea Research Institute of Bioscience and Biotechnology) ;
  • Kim, Chul-Ho (Metabolic Engineering Research Laboratory, Korea Research Institute of Bioscience and Biotechnology) ;
  • Rhee, Sang-Ki (Metabolic Engineering Research Laboratory, Korea Research Institute of Bioscience and Biotechnology, BioHoldings Inc., BVC #201, Korea Research Institute of Bioscience and Biotechnology)
  • Published : 2004.06.01
Download PDF
⟨ Previous Next ⟩

Abstract

The influences of impeller types on morphology and protein expression were investigated in a submerged culture of Aspergillus oryzae. The impeller types strongly affected mycelial morphology and protein production in batch and fed-batch fermentations. Cells that were cultured by propeller agitation grew in the form of a pellet, whereas cells that were cultured by turbine agitation grew in a freely dispersed-hyphal manner and in a clumped form. Pellet-grown cells showed high levels of protein production for both the intracellularly heterologous protein (${\beta}$-glucuronidase) and the extracellularly homologous protein (${\alpha}$-amylase). The feeding mode of the carbon source also influenced the morphological distribution and protein expression in fed-batch fermentation of A. oryzae. Pulsed-feeding mainly showed high protein expression and homogeneous distribution of pellet whereas continuous feeding resulted in less protein expression and heterogeneous distribution with pellet and dispersed-hyphae. The pellet growth with propeller agitation paralleling with the pulsed-feeding of carbon source showed a high level of protein production in the submerged fed-batch fermentation of recombinant A. oryzae.

Keywords

References

  1. Trends Biotechnol. v.15 Aspergillus as a host for heterologous protein production: The problem of protease Van den Hobergh,J.P.T.W.;P.J.I.Van de Vondervoort;L.Fraissinet-Tachet;J.Visser https://doi.org/10.1016/S0167-7799(97)01020-2
  2. Trends Biotechnol. v.20 Filamentous fungi as cell factories for heterologous protein production Punt,P.J.;N. van Biezen;A.Conesa;A.Albers;J.Mangnus;C. van den Hondel https://doi.org/10.1016/S0167-7799(02)01933-9
  3. Colloids Surfaces B: Biointerfaces v.24 Surface properties of Aspergillus oryzae spores investigated by atomic force microscopy Van der Aa, M.;M.Asther;Y.F.Dufrene https://doi.org/10.1016/S0927-7765(01)00277-6
  4. Adv. Biohem. Eng. v.60 Characterisation of mycelial morphology using image analysis Paul,G.C.;C.R.Thomas
  5. Biotechnol. Bioeng. v.19 The growth of moulds in the form of pellets-a literature review Metz,B.;N.W.F.Kossen https://doi.org/10.1002/bit.260190602
  6. Biotechnol. Prog. v.14 Influence of morphology on product formation in Aspergillus awamori during submerged fermentations Claus,L.J.;C.Leon;H.H.Jan https://doi.org/10.1021/bp980014x
  7. Biotechnol. Bioeng. v.68 Effect of biomass concentration and mycelial morphology on fermentation broth rheology Riley,G.L.;K.G.Tucker;G.C.Paul;C.R.Thomas https://doi.org/10.1002/(SICI)1097-0290(20000420)68:2<160::AID-BIT5>3.0.CO;2-P
  8. Food Technol. Biotechnol. v.39 The morphology of filamentous fungi in submerged cultivations as a bioprocess parameter Znidarsic,P.;A.Pavko
  9. Biotechnol. Prog. v.16 Increase heterologous protein production in Aspergillus niger fermentation through extracellular proteases inhibition by pelleted growth Jianfeng,X.;W.Liping;R.Darin;G.Tingyue;M.Y.Murray https://doi.org/10.1021/bp000006s
  10. Enzyme Microb. Technol. v.21 The relationship between pellet size and production of Mn(II) peroxidase by Phanerochaete chrysosporium in submerged culture Jimenz-Tobo,G.;M.J.Pennincky;R.Lejeune https://doi.org/10.1016/S0141-0229(97)00065-3
  11. Proc. Natl. Acad. Sci. USA v.83 β-Glucuronidase from Escherichia coli as gene fusion marker Jefferson,R.A.;S.M.Burgess;D.Hirsh https://doi.org/10.1073/pnas.83.22.8447
  12. Curr. Genet. v.40 Deletion analysis of the enoloase gene 9enoA) promoter from the filamentous fungus Aspergillus oryzae Toda,T.;M.Sano;M.Honda;O.Rimoldi;Y.Yang;M.Yamamoto;K.Kitamoto;T.Minetoki;K.Gomi;M.Machida https://doi.org/10.1007/s00294-001-0258-7
  13. Lett. Appl. Mirob. v.29 β-Glucuronidase gene from Escherichia coli is a functional reporter in the methylotrophic yeast Pichia pastoris Ayra-Pardo,C.;I.L.Montejo-Sierra;R.I.Vazquez-Padron;C.Garcia-Martinez https://doi.org/10.1046/j.1365-2672.1999.00610.x
  14. Anal. Chem. v.31 Use of dinitrosalicylic acid reagent for determination of reducing sugar Miller,G.L. https://doi.org/10.1021/ac60147a030
  15. Molecular Cloning: A Laboratory Manual(3rd ed.) Sambrook,J.;D.W.Russell
  16. Biotechnol. Bioeng. v.38 Inhibition of microbial growth and metabolism by excess turbulence Toma,M.K.;M.P.Ruklisha;J.J.Vanags;M.O.Zeltina;M.P.Leite;N.I.Galinina;U.E.Viesturs;R.P.Tengerdy https://doi.org/10.1002/bit.260380514
  17. Biotechnol. Prog. v.19 Macro-level and geneticlevel responses of Bacillus subtilis to shear stress Sahoo,S.;R.K.Verma;A.K.Suresh;K.K.Rao;J.Bellare;K.Suraishkumar https://doi.org/10.1021/bp034191w
  18. Appl. Microbiol. Biotechnol. v.43 Effect of agitation on growth and enzyme production of Trichoderma reesei in batch fermentation Lejeune,R.;G.V.Baron https://doi.org/10.1007/BF00172820
  19. Fungal Biotechnology Smith,J.E.;D.R.Ferry;B.Kristiansen
  20. Unit Operations of Chemical Engineering (5th ed.) McCabe,W.L.;J..Smith;P.Harriott
  21. Biotechnol. Bioeng. v.81 Effect of deletion of chitin synthase genes on mycelial morphology and culture viscosity in Aspergillus oryzae Muller,C.;K.Hansen;P.Szabo;J.Nielsen https://doi.org/10.1002/bit.10491
  22. Food Technol. Biotechnol. v.39 Submerged and solid-state phytase fermentation by Aspergillus niger: effects of agitation and medium viscosity on phytase production, fungal morphology and inoculum performance Papagianni,M.;S.E.Nokes;K.Filer
  23. Biotechnol. Bioeng. v.81 Pulsed feeding during fed-batch fungal fermentation lead to reduced viscosity without detrimentally affecting protein expression Bhargava,S.;M.P.Nandakumar;A.Roy;K.S.Wenger;M.R.Marten
  24. Biotechnol. Bioeng. v.82 Pulsed addition of limiting-carbon during Aspergillus oryzae fermentation lead to improved productivity of a recombinant enzyme Bhargava,S.;K.S.Wenger;M.R.Marten https://doi.org/10.1002/bit.10548