The Plant Pathology Journal

The Korean Society of Plant Pathology (한국식물병리학회)
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Characterization of Cucumber mosaic virus Isolated from Water Chickweed(Stellaria aquatica)

  • Park, Gug-Seoun (Department of Horticultural Environment, National Horticultural Research Institute, Rural Development Administration) ;
  • Kim, Jae-Hyun (Department of Horticultural Environment, National Horticultural Research Institute, Rural Development Administratio) ;
  • Kim, Jeong-Soo (Department of Horticultural Environment, National Horticultural Research Institute, Rural Development Administratio) ;
  • Park, Jang-Kyung (Division of Biological Environment, College of Agriculture and Life Science, Kangwon National University)
  • Published : 2004.06.01
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Abstract

A strain of Cucumber mosaic virus (CMV) was isolated from a weed, water chickweed (Stellaria aquatica), growing in the pepper field in Chunchon, Korea. This isolate, CMV-Sa, was differentiated from other CMVs based on biological properties and nucleotide sequence analysis of the coat protein (CP) gene. CMV-Sa showed different reactions to all the tested plants, except Capsicum annuum and Cucumis sativus, when compar-ed with those of CMV-Mf (subgroup I) and CMV-PaFM (subgroup II). Remarkably, in Nicotiana tabacum cvs. Samsun, Xanthi-nc and Ky-57, CMV-Sa induced local necrotic ring spots on the inoculated leaves and venal wave pattern and mosaic on the upper leaves. RNA analysis, serology, and RT-PCR of CP gene showed that CMV-Sa belonged to subgroup I of CMV. However, restriction enzyme analysis of the cDNA using AluI, HhaI, HincII, HindIII, HinfI and MspI showed that CMV-Sa was distinct from that of CMV-Mf. Based on comparison of the nucleotide of CP gene and deduced amino acid sequences between other CMV strains, CMV-Sa was closely related to CMV-Mf with 93.7% and 97.2 % identity, respectively.

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