Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Antioxidative and Antimutagenic Effects of Korean Buckwheat, Sorghum, Millet and Job기s Tears

한국산 메밀, 수수, 기장, 율무의 항산화효과 및 돌연변이억제효과

  • 곽충실 (서울대학교 체력과학노화연구소) ;
  • 임수진 (한남대학교 식품영양학) ;
  • 김성애 (한남대학교 식품영양학) ;
  • 박상철 (서울대학교 노화 및 세포사멸연구센) ;
  • 이미숙 (한남대학교 식품영양학과)
  • Published : 2004.07.01
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Abstract

Dietary intake of whole grains, vegetable and fruit is known to reduce the degenerative chronic diseases, such as cancer and cardiovascular diseases. Antioxidative and antimutagenic effects of the ethanol extract of Korean Millet, Buckwheat, Sorghum and Job's tears were examined by inhibition against iron-induced linoleate per-oxidation, DPPH (1,l-diphenyl-2-picrylhydrazyl) radical generation and MDA-BSA (malondialdehyde-bovine serum albumin) conjugation, and Ames test using Salmonella. Buckwheat showed the strongest antioxidative effect in three different systems among these four grains, but it showed the lowest antimutagenic effect. Sorghum was the second to Buckwheat in iron-induced linoleate peroxidation inhibition activity and DPPH radical scavenging activity, and showed very good direct-antimutagenic effect in 2-Nitrofluorene treated Salmonella Typhimurium TA98 and indirect-antimutagenic effect in 2-Anthramine treated Salmonella Typhimurium TA98 and TA100 with hepatic S9 mixture. Millet showed the strongest antimutagenic effect in Salmonella Typhimurium TA98 and TA 100 with or without S9. Buckwheat contained the highest total flavonoids and polyphenols, 1.14 mg/g and 3.71 mg/g, respectively. Total flavonoid content in these four grains was negatively correlated with $IC_{50}$/ for DPPH radical scavenging antioxidative effect significantly (r=-0.9924, p=0.0076), but not with antimutagenic effect.

한국산 메밀, 수수, 기장, 율무의 돌연변이억제효과와 항산화효과 및 총 flavonoid와 polyphenol 함량을 측정하였다. 이를 위하여 에탄을 추출시료를 얻은 후 Ames test 방법으로 돌연변이 억제효과를 검색하였고, Fe$^{2+}$로 유도된 지질과산화 억제율, DPPH 라디 칼 소거율 및 MDA-BSA 결합억제율을 측정하는 3가지 방법으로 항산화효과를 검정하고, 총 flavonoid와 polyphenol 함량을 측정한 결과를 요약하면 다음과 같다. 에탄올 추출시료의 $IC_{50}$/에 의한 지질과산화 억제효과를 비교한 결과 메밀(0.174 mg/ assay), 수수(0.241 mg/assay), 율무(1.891 mg/assay), 기장(2.873 mg/assay) 순이었으며 에탄을 추출시료의 $IC_{50}$/을 계산하여 DPPH 라디칼 제거능을 비교한 결과 메밀(0.264 mg/assay), 수수(2.135 mg/assay), 기장(2.902 mg/assay), 율무(4.667 mg/assay) 순이었다. 지질과산화물-단백질 결합 억제효과는 에탄을 추출시료의 $IC_{50}$/을 기준으로 비교한 결과 메밀(3.73 mg/assay), 율무(51.82 mg/assay), 기장(120.59 mg/assay), 수수(449.43mg/assay)의 순이었다. S. Typhimurium TA98에서 에탄올 추출물 4.5 mg/assay농도에서 2-Nitrofluorene에 의 한 직접돌연변이를 저해하는 비율은 수수(50.2%), 기장(20.3%), 메밀(18.5%), 율무(17.5%)의 순이었으며, S. Typhimurium TA100에서 sodium azide에 의한 직접돌연변이를 저해하는 비율은 기장(100%), 율무(78.6%), 수수(23.7%), 메밀(10.1%)의 순이었다. S. Typhimurium TA98 또는 TA100에서 에탄올 추출물이 2-Anthramine에 의한 간접돌연변이 억제효과는 기장, 수수, 율무, 메밀 모두 우수하였으며, 특히 기장과 수수의 효과가 매우 좋았다. 총 flavonoid 함량은 메밀(1.41mg/g), 수수(1.17 mg/g), 기장(0.96 mg/g), 율무(0.66 mg/g)순이었고, 총 polyphenol 함량은 메밀(3.71 mg/g), 수수(1.76 mg/g), 율무(1.7 mg/g), 기장(1.09 mg/g) 순으로 각각 DPPH 라디칼제거효과 및 지질과산화억제효과의 순과 일치하였다. 총 flavonoid 함량은 DPPH 라디칼 제거효과에 대한 $IC_{50}$/ 값과 높은 상관관계(r=-0.9924, p<0.01)를 나타내었다.

Keywords

References

  1. J Korean Soc Food Sci Nutr v.33 Antitumor and antimutagenic effect of proteinpolysaccarides from Polyporus umbellatus Lee JW;Shin YJ;Cho DJ;Lim HJ;Choi WE;Lee YK https://doi.org/10.3746/jkfn.2004.33.3.475
  2. J Korean Soc Food Sci Nutr v.33 Cytotoxicity and chemosensitizing effect of Camelia (Camelia japonica) tea extract Hwang EJ;Cha YU;Park MH;Lee JW;Lee SY https://doi.org/10.3746/jkfn.2004.33.3.487
  3. J Korean Soc Food Sci Nutr v.32 Purification and characterization of anti-coagulant activity fraction from persimmon stem Sa YS;Kim KA;Choi HS https://doi.org/10.3746/jkfn.2003.32.8.1323
  4. J Korea Soc Food Sci Nutr v.32 Inhibitory effect of hot-water extract of Paeonia japonica on oxidative stress and identification of its active components Jeong IY;Lee JS;Oh H;Jung U;Park HR;Jo SK https://doi.org/10.3746/jkfn.2003.32.5.739
  5. JAMA v.275 Vegetable, fruit and cereal fiber intake and risk of coronary heart disease among men Rimm EB;Ascherio A;Giovannucci E;Spiegleman D;Stampfer MS;Willet WC https://doi.org/10.1001/jama.275.6.447
  6. Nutr Cancer v.30 Whole grain intake and cancer: an expanded review and metaanalysis Jacobs DR;Marquart L;Slavin J;Kushi LH https://doi.org/10.1080/01635589809514647
  7. J Nat Prod v.63 Flavonoids as antioxidants Pietta PG https://doi.org/10.1021/np9904509
  8. J Agri Food Chem v.43 Evolution of antioxidant activity during kilning: Role of insoluble bound phenolic acids of barley and malt Maillard MN;Berset C https://doi.org/10.1021/jf00055a008
  9. Cereal Chem v.77 Effect of commercial processing on antioxidants in rice bran Lloyd BJ;Siebenmorgen TJ;Beers KW https://doi.org/10.1094/CCHEM.2000.77.5.551
  10. J Sci Food Agri v.81 Diferulates as structural components in soluble and insoluble cereal dietary fiber Bunzel M;Ralph J;Martia JM;Hatfield RD;Steinhart H https://doi.org/10.1002/jsfa.861
  11. Cancer Res v.45 Chemoprevention of cancer Wattenberg LW https://doi.org/10.1016/S0065-230X(08)60265-1
  12. J Agric Food Chem v.46 Catechines as antioxidants from buckwheat (Fagopyrum esculentum Moench) groats Watanabe M https://doi.org/10.1021/jf9707546
  13. Biochem Pharm v.32 Flavonoids a class of natural products of high pharmacological potency Havsteen B https://doi.org/10.1016/0006-2952(83)90262-9
  14. PhD thesis. Korea University Comparison of dietary fiber content in Korean foods and determining methods Kim EH
  15. Agri Chem Biotec v.39 Screening of antimutagenic activities from cereals and beans including rice Kang MY;Choi YH;Nam SH
  16. J Kor Soc Food Nutr v.20 Effect of buckwheat supplementation on blood glucose levels and blood pressure in rats Choe M;Kim JD;Park KS;Oh SY;Lee SY
  17. Kor J Nutr v.27 Effects of buckwheat on organ weight, glucose and lipid metabolism in streptozotocin-induced diabetic rats Lee JS;Son HS;Maeng YS;Chang YK;Ju JS
  18. Kor J Nutr v.28 Effects of buckwheat diet on serum glucose and lipid metabolism in NIDDM Lee JS;Lee MH;Chang YK;Ju JS;Son HS
  19. Planta Medicine v.60 Antitumor components isolated from the chinese herbal medicine Coix Lachryam-Jobi Numata M;Yamamoto A;Moribayash A;Yamada H https://doi.org/10.1055/s-2006-959500
  20. J Natural Products v.51 Anti-inflamatory activity of benzoxanoids from roots of Coix Lachryam-Jobi var. ma-yuen Otsuka H;Hirai Y;Nagao T;Yamasaki K https://doi.org/10.1021/np50055a009
  21. Planta Medicine v.52 Isolation and hypoglycemic activity of Coixans A,B,C, glycans of Coix lachyma-jobi L. var. ma-yuen seeds Takahashi M;Konno C;Hikino H https://doi.org/10.1055/s-2007-969074
  22. Folia Pharm Japan v.77 Behavioal and EEG effects of coixol (6-methoxy benzoxazolone) , one of the components in Coix lachryma-jobi L. var. ma-yuen Stapf Gomita Y;Ichimaru;Moriyama M;Fukamachi K;Uchikado A;Araki Y;Fukuda T;Koyama T https://doi.org/10.1254/fpj.77.245
  23. J Korean Soc Food Sci Nutr v.32 Effect of raw brown rice and Job's tear supplemented diet on serum and hepatic lipid concentrations, antioxidative system and immune function of rats Park J;Yang M;Jun HS;Lee JH;Bae HK;Park T https://doi.org/10.3746/jkfn.2003.32.2.197
  24. Kor J Oil Chem v.7 The effect of Job's tear diet on change of body lipid and tissue in rat Shin DK;Park CH;Lee YJ;Lee YO https://doi.org/10.3746/jkfn.2003.32.2.197
  25. J Kor Soc Food Sci Nutr Effect of millet powder and methanol extract on lipid synthesis-related enzymes or glucose-6-phosphatase activity in rats. P-2 Poster session in Annual meeting Chung S;Cho S;Lee H;Kim G;Ha T
  26. Korean J Food Sci Technol v.29 Compositions of Opumtia fiscus-medica Lee YC;Hwang KH;Han DH;Kim SD
  27. Kor J Nutr v.21 Effect of Coix on plasma cholesterol and lipid metabolism in rats Park Y;Lee YS;Suzuki H
  28. Standard Food Analysis Chae SK;Kang GS;Ma SJ;Bang KW;Oh MW;Oh SH
  29. Methods Enzymol v.299 Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteau reagent Singleton VL;Orthofer R;Lamuela-Raventos RM https://doi.org/10.1016/S0076-6879(99)99017-1
  30. Mutat Res v.113 Revised methods for the Salmonella mutagenicity test Maron DM;Ames BN https://doi.org/10.1016/0165-1161(83)90010-9
  31. Short-term test, systems for detecting carcinogens Factors modulating mutagenicity in microbial test Matsushima T;Sugimura T;Nagao M;Yahagi T;Shirai A;Sawamura M;Norphth KH(ed.);Garner RC(ed.)
  32. Free Rad Bio Med v.19 Flavonoids as antioxidant agents: Importance of their interaction with biomembranes Antorella S;Mario S;Maria L;Daniela M;Francesco B;Francesco C https://doi.org/10.1016/0891-5849(94)00240-K
  33. J Lipid Res v.10 Ascorbic acid and copper in linoleate oxidation: Measurement of oxidation by ultraviolet spectrophotometry and the thiobarbituric acid test Gotteried H;Dunkley WL
  34. Nature v.26 Antioxidant determination by the use of a stable radical Blois M.S.
  35. PhD thesis. Seoul National University Effect of polyamine on modification of biomedics by aldehydes Park Y.H.
  36. Anal Biochem v.72 A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding Bradford M.M. https://doi.org/10.1016/0003-2697(76)90527-3
  37. Tetrahedron Letters v.28 Cleavage and oligomerization of malondialdehyde under physiological conditions Gomez-Sanchez A;Hermonsin I;Mayo I
  38. J Agric Food Chem v.36 Chemical studies on novel rice hull antioxidants Ramarathanam N;Osawa T;Namiki M;Kawakishi S;Ohshima K https://doi.org/10.1021/jf00082a014
  39. Proc Sym On Physiologically Active Substances in Plant Foods Physiological effects of phytoestrogens in relation to cancer and other human health risks Cassidy A
  40. J Food Sci v.48 Comparative GLC-MS analysis of phenolic acids of selected tree nuts Senter SD;Horvat RJ;Forbus WR https://doi.org/10.1111/j.1365-2621.1983.tb14902.x
  41. J Agric Food Chem v.30 Free, esterified, and insoluble-bound phenolic acids. 3. Composition of phenolic acids in cereal and potato flours Sosulski F;Krygier K;Hogge L https://doi.org/10.1021/jf00110a030
  42. J Agric Food Chem v.48 Antioxidant activity and total phenolics in selected cereal grains and their different morphological fractions Zielinski H;Kozlowska H https://doi.org/10.1021/jf990619o
  43. J Agric Food Chem v.50 Antioxidant activity of grains Adom KK;Liu RH https://doi.org/10.1021/jf0205099
  44. Kor J Gerontol v.13 Antimutagenic and antioxidative effects of ethanol extracts from garlic, ginger, green onion and red pepper Lee MS;Lim SJ;Kim SA;Woo MK;Kwak CS;Park SC
  45. Cancer v.64 Increased lipid peroxidation in malignant tissues of patients with cololectal cancer Otamari T;Sjodahl R https://doi.org/10.1002/1097-0142(19890715)64:2<422::AID-CNCR2820640214>3.0.CO;2-2
  46. Biosci Biotechnol Biochem v.65 Effects of buckwheat in a renal ischemia-reperfusion model Yokozawa T;Fujii H;Kosuna K;Nonaka G https://doi.org/10.1271/bbb.65.396
  47. Lor J Food Sci Tech v.32 Tyrosinase-inhibitory and radical scavenging activities from the seeds of Coix lachryma-jobi L Kim JK;Lee HS

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  48. Beneficial Effect of Anti-obese Herbal Medicine Mixture with Chitosan in High Fat Diet-induced Obese Rats vol.14, pp.4, 2004, https://doi.org/10.3746/jfn.2009.14.4.290
  49. 국내 유통 혼합잡곡 제품의 품질 및 항산화 특성 vol.30, pp.1, 2004, https://doi.org/10.9799/ksfan.2017.30.1.031
  50. 메밀분말을 첨가한 닭고기 소시지의 품질 특성 vol.44, pp.2, 2004, https://doi.org/10.5536/kjps.2017.44.2.135
  51. 국내 기장 및 수수의 바이러스병 발생 조사 vol.23, pp.3, 2004, https://doi.org/10.5423/rpd.2017.23.3.262
  52. Erect Plant Type and Medium Maturing Proso millet (Panicum miliaceum L.) Variety 'Geumsilchal' vol.50, pp.3, 2018, https://doi.org/10.9787/kjbs.2018.50.3.296
  53. 전기방사에 의해 제조된 수수 추출물 함유 폴리비닐알코올 복합 나노 섬유의 생리활성 특성 vol.31, pp.4, 2004, https://doi.org/10.5764/tcf.2019.31.4.323
  54. 잡곡 선별을 위한 물성 측정에 관한 연구 vol.21, pp.10, 2004, https://doi.org/10.5762/kais.2020.21.10.354
  55. Functional Compounds and Physiological Activities of Foxtail Millet (Setaria italica) and Finger Millet (Eleusine coracona) vol.50, pp.11, 2004, https://doi.org/10.3746/jkfn.2021.50.11.1152