Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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DPPH Radical Scavenger Activity and Antioxidant Effects of Cham-Dang-Gui (Angelica gigas)

참당귀(Angelica gigas)의 DPPH Radical 소거 활성과 항산화 효과

  • 강순아 (건국대학교 분자생명공학과 생명분자정보학센터) ;
  • 한진아 (경희대학교 동서의학대학원 임상영양학전) ;
  • 장기효 (삼척대학교 식품영양학) ;
  • 조여원 (경희대학교 동서의학대학원 임상영양학전공)
  • Published : 2004.08.01
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Abstract

This study was carried out to evaluate the free radical scavenging effect and antioxidant effect of Cham-Dang-Gui (Angelica gigas) on cyclophosphamide (CYP) injected rats. Rats were divided into five groups： CON (normal group), ANS (CYP-injected and normal diet group), AND (CYP-injected and normal diet and Cham-Dang-Gui-treated group), ALS (CYP-injected and low iron diet group), and ALD (CYP-injected and low iron diet and Cham-Dang-Gui-treated group). CYP (30 mg/kg) was intraperitoneally injected to rats for early 3 days. Saline or Cham-Dang-Gui was administrated orally for entire experimental period. DPPH radical scavenger activity was measured by DPPH method, it was shown higher in methanol extract (81.5％) than in water extract (66.3％) of Cham-Dang-Gui. We observed the preventive effects of Cham-Dang-Gui on lipid oxidation of liver and protein oxidation of plasma. Hepatic SOD and catalase activities were significantly higher in CYP-injected group (ANS) than CON group, but SOD activity was slightly lowered in Cham-Dang-Gui treated group than CYP-injected group (ANS). These results suggest that extract of Cham-Dang-Gui could be useful for functional materials to reduce the oxidation of lipids and protein induced by free radicals.

본 연구의 결과를 요약하면 참당귀추출물 1 mg/mL의 농도에서 열수추출물의 free radical소거 효과는 메탄올추출 물보다 높게 나타났으며 농도 의존적으로 DPPH free radical 소거 효과가 상승하는 것을 관찰하였다. 또한 참당귀 투여에 의하여 화학요법으로 발생된 지질과산화물의 생성을 감소시켰으며, 단백질 산화를 유의적으로 억제함으로써 참당귀추출물은 CYP를 투여로 생성된 free radical을 감소시킴으로써 독성을 감소시켜, 혈액학적 빈혈지표가 더 감소되는 것을 막았다고 사료되며, 항산화제로서의 가능성이 있는 한약재로서 활용성이 높을 것으로 사료된다 항산화효소인 SOD 활성은CYP투여에 의하여 활성이 증가하였으며 참당귀추출물의 투여에 의한 효과가 나타나지 않았으나 감소하는 경향을 보였고 catalase 활성은 CYP 투여에 의하여 활성이 증가하였으나 참당귀의 효과는 차이가 없었다. 결론적으로 참당귀추출물은 free radical 소거능을 갖고, 화학요법으로 발생된 지질과산화물의 생성을 낮춰 화학요법에 의한 부작용으로 발생되는 혈액학적 수치의 감소를 막음으로써 항산화제로의 활용성을 기대해본다.

Keywords

References

  1. Medical college of Seoul University. 1993. Hematology. Seoul National Univ Publish., Seoul.
  2. Ma JY, Ha CS, Sung HJ, Zee OP. 2000. Hemopoietic effects of Rhizoma Rehmanniae Preparata on Cyclophosphamideinduced pernicious anemia in rats. Kor J Pharmacogn 31: 325-334.
  3. Tas F, Eralp Y, Basaran M, Sakar B, Alici S, Argon A, Bulutlar G, Camlica H, Aydiner A, Topuz E. 2002. Anemia in oncology practice: relation to diseases and their therapies. Am J Clin Oncol 25: 371-379. https://doi.org/10.1097/00000421-200208000-00011
  4. Das UB, Mallick M, Debnath JM, Ghosh D. 2002. Protective effect of ascorbic acid on cyclophosphamide-induced testicular gametogenic and androgenic disorders in male rats. Asian J Androl 4: 201-207.
  5. Ghosh D, Das UB, Misro M. 2002. Protective role of alphatocopherol- succinate (provitamin E) in cyclophosphamideinduced testicular gametogenic and steroidogenic disorders: a correlative approach to oxidative stress. Free Radic Res 36: 1209-1218. https://doi.org/10.1080/1071576021000016472
  6. Kaya H, Oral B, Ozguner F, Tahan V, Babar Y, Delibas N. 1999. The effect of melatonin application on lipid peroxidation during cyclophosphamide therapy in female rats. Zentralbl Gynakol 121: 499-502.
  7. Feusner J, Hastings C. 2002. Recombinant human erythropoietin in pediatric oncology: a review. Med Pediatr Oncol 39: 463-468. https://doi.org/10.1002/mpo.10187
  8. Valley AW. 2002. Overview of cancer-related anemia: focus on the potential role of Darbepoetin alfa. Pharmacotherapy 22 (9 Pt 2): 150S-159S. https://doi.org/10.1592/phco.22.14.150S.33400
  9. Kim DU, Jin JL, Lee JU, Han CH, Min SS, Kim HG, Park JW, Kim CC, Kim DI. 1992. Remedy of mixed immune in aplastic anemia patient. Korean J Hematology 27: 233-237.
  10. Cha BH, Oh SH, Yu CJ, Yang CY, Kim GY. 1992. Recombinant human granulocyte colony stimulation factor treatment of apriority agranulocytosis: 2 Case Report. Korean J Hematology 27: 325-329.
  11. Lee SI, Ahn DG, Shin MG, No SH, Lee YJ, Kim KH. 1982. Clinical Applied of Herb. Seung-Bo publishing company, Seoul. p 266-384.
  12. Liu XL. 1984. Twelve cases of aplastic anemia treated mainly by ready Chinese drugs. Cung/ TSA Chih 25: 759-760.
  13. Bradley RR, Cunniff PJ, Pereira BJ, Jaber BL. 1999. Hematopoietic effect of Radix angelicae sinensis in a hemodialysis patient. Am J Kidney Dis 34: 349-354. https://doi.org/10.1016/S0272-6386(99)70367-7
  14. Kim HS, Heu IH, Lee SJ, Ahn HS. 1989. Immunologic characteristic on extract of old anter. Kor J Pharmacogn 38: 806-813.
  15. Park KJ, Hong SB, Kim EH, Ma JY, Eun YA, Kim HS. 1998. Hemopoietic effects of deer blood on cyclophosphamide induced pernicious anemia. Kor J Pharmacogn 29: 283-292.
  16. Han DS. 1988. Herbology. Dong-Myoung publicating company, Seoul. p 201-202.
  17. Han JA, Jang KH, Kang SA, Choue R. 2003. Hematological Effects of water extracts of Cham-Dang-Gui on cyclophosphamide induced anemic rat. Korean J Nutrition 36: 1013-1021.
  18. Kim SH, Lim JS. 1999. Effect of Korean traditional medicine on murine hematopoiesis (I) - Regulation of hematopoietic cytokine expression. Korean J Immunol 21: 165-174.
  19. Colvin M. 1982. The alkylating agents. In Pharmacological principles of cancer treatment. Chabner B, ed. Saunders, Philadelphia. p 276.
  20. Mortensen SR, Chanda SM, Hooper MJ, Padilla S. 1996. Maturational differences inchlorphyrifos-oxonase activity may contribute to age-related sensitivity to chlorpyrifos. J Biochem Toxicol 11: 279-287. https://doi.org/10.1002/(SICI)1522-7146(1996)11:6<279::AID-JBT3>3.0.CO;2-H
  21. Lim JP, Song YC, Kim JW, Ku CH, Eun JS, Leem KH, Kim DK. 2002. Free radical scavengers from the heartwood of Juniperus chinensis. Arch Pharm Res 25: 449-452. https://doi.org/10.1007/BF02976600
  22. Shinnburber RO, Yu TC. 1958. Characterization of the red pigment formed in 2-thiobarbituric acid determination of oxidation rancibiliry. Food Res 23: 620-625.
  23. Lee YK. 2000. Effects of dietary fatty acids and protein sources on lipid metabolism and DMBA induced mammary tumors in rats. PhD Dissertation. HanYang University.
  24. Buege JA, Aust SD. 1978. Microsomal lipid peroxidation. Method Enzymol 52: 302-306. https://doi.org/10.1016/S0076-6879(78)52032-6
  25. Oliver CN, Ahn B, Moerman EJ, Goldstein S, Stadtman ER. 1987. Age-related changes in oxidized proteins. J Biol Chem 262: 5483-5492.
  26. Marklund S. 1984. Pyrogallol antioxidant. In CRC hanbook of methods for oxygen radical research. CRC Press, New York. p 243.
  27. Marklund S, Marklund G. 1974. Involvement of superoxide anion radical in the oxidation of pyrogallol and a convenient assay for superoxide dismutase. Eur J Biochem 47: 468-471.
  28. Aebi HE. 1984. Catalase. In Methods in enzymatic analysis. 3rd ed. Verlag Chemie, Weinheim NY. Vol 3, p 273-285.
  29. Claiborne A. 1984. Catalase activity. In CRC hanbook of methods for oxygen radical research. CRC Press, New York. p 283.
  30. Harman D. 1987. The free radical theory of aging. In Mordern biological theories of aging. Warner HR, Butler RN, Sprott RL, Schneider EL, eds. Raven Press, NY. p 89.
  31. Kim SM, Cho YS, Kim EJ, Bae MJ, Han JP, Lee SH, Sung SK. 1998. Effect of hot water extracts of Salvia miltiorrhiza Bge., Prunus persica Stokes, Angelica gigas Nakai and Pinus strobus on lipid oxidation. J Korean Soc Food Sci Nutr 27: 399-405.
  32. Choi Y, Kim M, Shin JJ, Park JM, Lee J. 2003. The antioxidant activities of the some commercial teas. J Korean Soc Food Sci Nutr 32: 723-727. https://doi.org/10.3746/jkfn.2003.32.5.723
  33. Kaya H, Oral B, Ozguner F, Tahan V, Babar Y, Delibas N. 1999. The effect of melatonin application on lipid peroxidation during cyclophosphamide therapy in female rats. Zentralbl Gynakol 121: 499-502.
  34. Kim SJ, Song BG, Lee EJ, Kim HG, Kim JG. 2000. The effect of Angellica gigas on the relaxation of vascular smooth muscle and the expression of iNOS. Korean J Immunol 20: 60-67.
  35. Kang SY, Lee KY, Park MJ, Kim YC, Markelonis GJ, Oh TH, Kim YC. 2003. Decursin from Angelica gigas mitigates amnesia induced by scopolamine in mice. Neurobiol Learn Mem 79: 11-18. https://doi.org/10.1016/S1074-7427(02)00007-2
  36. Hur SK, Kim SS, Heo YH, Ahn SM, Lee BG, Lee SK. 2001. Effect of the grapevine shoot extract on free radical scavenging activity and inhibition of pro-inflammatory mediator production in raw 264.7 macrophages. J Applied Pharmacology 9: 188-193.
  37. Roberforid M, Calderon PB. 1995. Free radicals and oxidation phenomena in biological system. Marcel Dekker, Inc, New York.
  38. Chang NS, Ryu SM. 2001. Antioxidative effects of green tea powder diet against ethanol-induced oxidative damage in rat brain region. Kor J Nutr 34: 525-531.
  39. Rekha PS, Kuttan G, Kuttan R. 2001. Effect of Brahma Rasayana on antioxidant systems and cytokine levels in mice during cyclophosphamide administration. J Exp Clin Cancer Res 20: 219-223.

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