Biotechnology and Bioprocess Engineering:BBE

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Decolorization of Melanin by Lignin Peroxidase from Phanerochaete chrysosporium

  • Woo, Sung-Hwan (Department of Biological Engineering, Inha University) ;
  • Cho, Jeung-Suk (Department of Biological Engineering, Inha University) ;
  • Lee, Baek-Seok (Department of Biological Engineering, Inha University) ;
  • Kim, Eun-Ki (Department of Biological Engineering, Inha University)
  • Published : 2004.07.01
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Abstract

Melanin was decolorized by lignin peroxidase from Phanerochaete chrysosporium. This decolorization reaction showed a Michaelis-Mentens type relationship between the decolorization rate and concentration of two substrates: melanin and hydrogen peroxide. Kinetic constants of the decolorization reaction were 0.1 OD$\sub$475//min ($V_{max}$) and 99.7 mg/L ($K_{m}$) for melanin and 0.08 OD$\sub$475//min ($V_{max}$) and 504.9 ${\mu}$M ($K_{m}$) for hydrogen peroxide, respectively. Depletion of hydrogen peroxide interrupted the decolorization reaction, indicating the essential requirement of hydrogen peroxide. Pulsewise feeding of hydrogen peroxide continued the decolorizing reaction catalyzed by lignin peroxidase. These results indicate that enzymatic decolorization of melanin has applications in the development of new cosmetic whitening agents.

Keywords

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