Biotechnology and Bioprocess Engineering:BBE

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
권호 표지

Statistical Optimization of Medium Composition for Growth of Leuconostoc citreum

  • Kim, Hyun (Department of Food Science and Technology, Research Center for Bioresource and Health, Chungbuk National University) ;
  • Eom, Hyun-Ju (Department of Food Science and Technology, Research Center for Bioresource and Health, Chungbuk National University) ;
  • Lee, Jun-soo (Department of Food Science and Technology, Research Center for Bioresource and Health, Chungbuk National University) ;
  • Han, Jin-soo (Orient Inc) ;
  • Han, Nam-Soo (Department of Food Science and Technology, Research Center for Bioresource and Health, Chungbuk National University)
  • Published : 2004.07.01
Download PDF
⟨ Previous Next ⟩

Abstract

Leuconostoc citreum is one of the representative strains of Leuconostoc spp. that show fast growth rates in fermented vegetables. Sequential experimental designs including the Plackett-Burman design, fractional factorial design, steepest ascent analysis, central composite design and response surface methodology were introduced to optimize and improve the medium for L. citreum. Fifteen medium ingredients were examined and glucose ($20 g/\ell$), yeast extract ($12.5g/\ell$), sodium acetate trihydrate ($6.12g/\ell$), potassium phosphate ($42.55g/\ell$), and dibasic ammonium citrate ($4.12g/\ell$), were chosen as the best components to give a critical and positive effect for cell-growth. The biomass was increased to ($2.79g/\ell$), (169%), compared to the $1.65g/\ell$ in MRS medium.

Keywords

References

  1. Bio/Technology v.10 Recombinant protein expression in high cell density cultures of Escherichia coli Lee, S. Y.;H. Blanch https://doi.org/10.1038/nbt1292-1550
  2. Trends Biotechnol. v.14 High cell-density culture of Escherichia coli. Lee, S. Y. https://doi.org/10.1016/0167-7799(96)80930-9
  3. Appl. Microbiol. Biotechnol. v.51 no.4 High-cell-density cultivation of microorganisms Riesenberg, D.;R. Guthke https://doi.org/10.1007/s002530051412
  4. Biotechnol. Bioprocess Eng. v.7 Periodic change in DO concentration for efficient poly-β-hydroxy-butyrate production using temperature-inducible recombinant Escherichia coli with proteome analysis Rahman, N. A. A.;Y. Shirai;K. Shimizu;M. A. Hassan https://doi.org/10.1007/BF02932837
  5. Biotechnol. Bioprocess Eng. v.8 The soluble expression of the human renin binding protein using fusion partners: A comparison of ubiquitin, thioredoxin, maltose binding protein and NusA Lee, C.;S. G. Lee;S. Takahashi;B. G. Kim https://doi.org/10.1007/BF02940262
  6. List of the substances that are the subject of each GRAS Notice and the file number that FDA has assigned to the notice(GRAN No.) U. S. Food and Drug Administraion
  7. M. S. Thesis, Chungbuk National University Isolation of Psychrotrophic Leuconostoc mesenteroides Producing Highly Active Dextransucrase and Application to Lactate-fermented Foods Eom, H. J.
  8. J. Korean Soc. Food Sci. Nutr. v.25 Selective media for isolation and enumeration of lactic acid bacteria form Kimchi Lee, M.;W. Park;H. Kang
  9. J. Ind. Microbiol. v.11 Response surface methods for optimizing Saccharopolyspora spinosa, a novel macrolide producer Strobel, R. J.;W. M. Nakatsukasa https://doi.org/10.1007/BF01583684
  10. Starch v.38 Development and field confirmation of a mathematical model for amylogucosidase/pullanase saccharification Swanson, T. R.;J. O. Carroll;R. A. Britto;D. J. Duhart https://doi.org/10.1002/star.19860381106
  11. J. Appl. Bacteriol. v.43 Use of response surface methodology for the rapid optimization of microbiological media Maddox, I. S.;S. H. Richert
  12. Appl. Microbiol. Biotechnol. v.48 Optimization of microbial transglutarninase production using experimental designs Goulas, P.;M. Junqua;R. Duran;C. Gancet https://doi.org/10.1007/s002530051124
  13. Biotechnol. Appl. Biochem. v.26 Application of a statistical technique to the production of Saccharomyces cerevisiae (baker's yeast) Alpbaz, M.;N. Bursali;S. Ertunc;B. Akay
  14. Enzyme Microb. Technol. v.13 Elucidation and optimization of the medium constituents controlling antibiotic production by the cyanobacterium Nostoc muscorum Bloor, S.;R. R. England
  15. Biometrika v.33 The design of optimum multifactorial experiments Plackett, R. L.;J. P. Burman https://doi.org/10.1093/biomet/33.4.305
  16. Response surface methodology Khuri, A. I.;J. A. Cornell
  17. Experimental Design in Biotechnology Haaland, P. D.
  18. Response Surface Methodology: Process and Product Optimization Using Designed Experiments Myers, R. H.;D. C. Montgomery
  19. Technometrics v.2 Some new three level design for the study of quantitative variables Box, G. E. P.;D. W. Behnken
  20. Enzyme Microb. Technol. v.30 Optimization of medium composition for the production of elastase by Bacillus sp. EL31410 with response surface methodology Chen, Q. H.;G. Q. He;A. M. Ali. Mokhtar https://doi.org/10.1016/S0141-0229(02)00028-5