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The Pharmaceutical Society of Korea (대한약학회)
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Involvement of Nitric Oxide During In Vitro Fertilization and Early Embryonic Development in Mice

  • Kim, Bo-Hyun (Eden Obstetric & Gynecology Clinic) ;
  • Kim, Chang-Hong (Eden Obstetric & Gynecology Clinic) ;
  • Jung, Kyu-Young (Department of Pharmacology, Wonkwang University School of Medicine) ;
  • Jeon, Byung-Hun (Department of Pathology, College of Oriental Medicine, Wonkwang University) ;
  • Ju, Eun-Jin (Department of Biological science, College of Natural Sciences and Medicinal Resources Research Center, Wonkwang University) ;
  • Choo, Young-Kug (Department of Biological science, College of Natural Sciences and Medicinal Resources Research Center, Wonkwang University)
  • Published : 2004.01.01
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Abstract

Nitric oxide (NO) has emerged as an important intracellular and intercellular messenger, controlling many physiological processes and participating in the fertilization process via the autocrine and paracrine mechanisms. This study investigated whether nitric oxide synthase (NOS) inhibitior (L-NAME) and L-arginine could regulate in vitro fertilization and early embryonic development in mice. Mouse epididymal spermatozoa, oocytes, and embryos were incubated in mediums of variable conditions with and without L-NAME or L-arginine (0.5, 1, 5 and 10mM). Fertilization rate and early embryonic development were significantly inhibited by treating sperms or oocytes with L-NAME (93.8% vs 66.3%,92.1% vs 60.3%), but not with L-arginine. In contrast, fertilization rate and early embryonic development were conspicuously reduced when L-NAME or L-arginine was added to the culture media for embryos. Early embryonic development was inhibited by microinjection of L-NAME into the fertilized embryosin a dose-dependent manner, but only by high concentrations of L-arginine. These results suggest that a moderate amount of NO production is essential for fertilization and early embryo development in mice.

Keywords

References

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