Archives of Pharmacal Research

  • 제27권8호
  • /
  • Pages.850-856
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  • 2004
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  • 0253-6269(pISSN)
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  • 1976-3786(eISSN)
대한약학회 (The Pharmaceutical Society of Korea)
권호 표지

Tissue Transglutaminase is Not Involved in the Aggregate Formation of Stably Expressed $\alpha$-Synuclein in SH-SY5Y Human Neuroblastoma Cells

  • Suh, Myung-Duk (Department of Pharmacology, College of Medicine, Kangwon National University) ;
  • Park, Chang-Ha (Department of Pharmacology, College of Medicine, Kangwon National University) ;
  • Kim, Sung-Soo (Department of Pharmacology, College of Medicine, Kangwon National University) ;
  • Kil, Myeng-Og (Department of Pharmacology, College of Medicine, Kangwon National University) ;
  • Lee, Geon-Hee (Department of Pharmacology, College of Medicine, Kangwon National University) ;
  • Johnson, Gail V. W. (Department of Pharmacology, College of Medicine, Kangwon National University) ;
  • Chun, Wan-Joo (Department of Psychiatry, University of Alabama at Birmingham)
  • 발행 : 2004.08.01
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초록

Intraneuronal deposition containing $\alpha$-synuclein is implicated in the pathogenesis of synuclein-opathies including Parkinsons disease (PD). Although it has been demonstrated that cytoplas-mic inclusions of wild type $\alpha$-synuclein are observed in the brain of PD patients and that $\alpha$-synuclein mutations such as A30P and A53T accelerate aggregate formation, the exact mech-anism by which $\alpha$-synuclein forms insoluble aggregates is still controversial. In the present study, to understand the possible involvement of tissue transglutaminase (tTG) in aggregate formation of $\alpha$-synuclein, SH-SY5Y cell lines stably expressing wild type or mutant (A30P or A53T) $\alpha$-synuclein were created and aggregate formation of $\alpha$-synuclein was observed upon activation of tTG. The data demonstrated that $\alpha$-synuclein negligibly interacted with tTG and that activation of tTG did not result in the aggregate formation of $\alpha$-synuclein in SH-SY5Y cells overexpressing either wild type or mutant $\alpha$-synuclein. In addition, $\alpha$-synuclein was not modi-fied by activated tTG in situ. These data suggest that tTG is unlikely to be a contributing factor to the formation of aggregates of $\alpha$-synuclein in a stable cell model.

키워드

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