Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
권호 표지
DOI QR코드

DOI QR Code

Antimicrobial Effect of the Extracts of Cactus Chounnyouncho(Opuntia humifusa) against Food Borne Pathogens

병원성 식중독 미생물에 대한 천년초 선인장 추출물의 항균 활성

  • Lee, Kyung-Seok (Dept. of Food and Biotechnology, Hoseo University) ;
  • Kim, Min-Gue (Dept. of Food and Biotechnology, Hoseo University) ;
  • Lee, Ki-Young (Dept. of Food and Biotechnology, Hoseo University)
  • 이경석 (호서대학교 식품생물공학과) ;
  • 김민규 (호서대학교 식품생물공학과) ;
  • 이기영 (호서대학교 식품생물공학과)
  • Published : 2004.10.01
Download PDF
⟨ Previous Next ⟩

Abstract

The 70% ethanol extract from cactus Chounnyouncho (Opuntia humifusa) was fractionated subsequently by hexan, chloroform, ethyl acetate, buthanol and water. The ethyl acetate fraction exhibited strong antimicrobial activities by paper disk diffusion method on the five strains of food born bacteria Bacillus subtilis, Staphylococcus aureus subsp. aureus, Escherichia coli, Salmonella typhimurium and Pseudomonas fluorescens. It has a heat resistance to show inhibitory effect after heat treatment at 11$0^{\circ}C$ for 1 hr. The ethyl acetate fraction showed almost perfect growth inhibition at over 700 ppm on the tested strains.

본 연구에서는 천년초 손바닥 선인장 유기용매 추출물의 항균성을 비교, 검토하고자 하였다. 70% ethanol로 추출하여 hexan, chloroform, ethyl acetate, buthanol, water로 순차 분획하여 ascorbic acid, benzoic acid와 항균효과를 비교하여 보았다. Escherichia coli 등 Gram positive bacteria 2종, Gram negative bacteria 2종, yeast 1종 등 총 6개의 병원성 식중독 미생물에 대해 항균효과를 검토한 결과 ethyl acetate 분획물이 가장 우수한 항균효과를 나타내었고 이는 동량의 benzoic acid보다 월등히 우수하였다. Gram positive bacteria와 Gram negative bacteria에 대해 유의적 차이가 없는 비슷한 항균효과를 보여주었으나 bacteria에 비해 yeast에서 항균효과는 낮게 나타났다.

Keywords

References

  1. Todd ECD. 1989. Preliminary estimates of costs of food borne disease in the United States. J Food Prot 1: 595-601
  2. Scott VN. 1988. Safety considerations for new generation refrigerated foods. Dairy Food Environ Sanitation 1: 5-10
  3. Frank JF. 1990. Control of L. monocytogenes in food processing environments. J Korean Soc Food Nutr 19: 491-493
  4. Park SK, Park JC. 1994. Antimicrobial activity of extracts and coumaric acid isolated from Artemisia princeps var. orientalis. Korean J Biotechnol Bioeng 9: 506-511
  5. Kim YS, Kim MN, Kim JO, Lee JH. 1994. The effect of hot water-extract and flavor compounds of mugwort on microbial growth. J Korean Soc Food Nutr 23: 994-1000
  6. Brane AL. 1975. Toxicological and biochemistry of butylated hydroxyanusole and butylated hydroxytoluene. JAOCS 52: 59-63 https://doi.org/10.1007/BF02901825
  7. Beuchat LR, David AG. 1989. Antimicrobials occurring naturally in foods. J Food Technology 43: 134-142
  8. Maria BZ, Lamar SR, Jose SM, Minam LS, Amaldo RL. 1984. Volatile sulfides of the amazonian garlic bush. J Agric Food Chem 32: 1009-1010 https://doi.org/10.1021/jf00125a014
  9. Jamal NB, Ibrahim AW. 1994. Citric acid and antimicrobial affect microbiological stability and quality of tomato juice. J Food Sci 59: 130-134 https://doi.org/10.1111/j.1365-2621.1994.tb06916.x
  10. 이시진. 1994. 본초강목. 의성당, 서울
  11. Shin TK, Lee SJ, Kim SJ. 1998. Effects of Opuntia ficus- indica extract on the activation of immune cells with special reference to autoimmune disease models. Korean J Vet Pathol 2: 31-35
  12. Seo KL, Yang KH, Shim KH. 1999. Antimicrobial and antioxidative activities of Opuntia ficus-indica var. saboten extracts. Korean J Postharvest Sci Technol 6: 355-359
  13. Kim MS, Lee DC, Hong JE, Chang KS, Cho HY, Kwon YK, Kim HY. 2000. Antimicrobial effects of ethanol extracts from Korean and Indonesian plants. Korean J Food Sci Technol 32: 949-958
  14. Lee OH, Jeong SH, Son JY. 2004. Antimicrobial activity of clove extract by extraction solvents. J Korean Soc Food Sci Nutr 33: 494-499 https://doi.org/10.3746/jkfn.2004.33.3.494
  15. Kim JS, Gu GM, Jeong YH, Yang JG, Lee GG. 2004. Antimicrobial activities of Zanthoxylum schinifolium extract against Vibrio parahaemolyticus. J Korean Soc Food Sci Nutr 33: 500-504 https://doi.org/10.3746/jkfn.2004.33.3.500
  16. Yun JW, Yu MY, Park BG, Lee MG, O DH. 2004. Antimicrobial effect of ethanol extracts of Quercus spp. against foodborne pathogens. J Korean Soc Food Sci Nutr 33: 463-468 https://doi.org/10.3746/jkfn.2004.33.3.463

Cited by

  1. Quality Changes of Sulgidduk Added Cheonnyuncho (Oputia Humifusa) Fruit Powder during Storage vol.29, pp.5, 2013, https://doi.org/10.9724/kfcs.2013.29.5.501
  2. Structural Analysis of Anti-metastatic Polysaccharides Isolated from Opuntia humifusa vol.40, pp.2, 2011, https://doi.org/10.3746/jkfn.2011.40.2.214
  3. A Study on Biological Activities of Opuntia humifusa Cladode Extracts vol.55, pp.2, 2012, https://doi.org/10.3839/jabc.2011.068
  4. Dietary fiber, mineral elements profile and macronutrients composition in different edible parts of Opuntia microdasys (Lehm.) Pfeiff and Opuntia macrorhiza (Engelm.) vol.64, pp.1, 2015, https://doi.org/10.1016/j.lwt.2015.05.011
  5. Physiological Activities of Opuntia humifusa Petal vol.27, pp.5, 2011, https://doi.org/10.9724/kfcs.2011.27.5.523
  6. The Antibacterial Effects of Backryeoncho(Opuntia ficus-indica var. saboten) Extracts as Applied to Kimchi Fermentation with Lactic Acid Bacteria and Food Poisoning Bacteria vol.20, pp.6, 2011, https://doi.org/10.5934/KJHE.2011.20.6.1213
  7. Antioxidant and Inflammatory Mediators Regulation Effects of the Roots of Opuntia humifusa vol.57, pp.1, 2014, https://doi.org/10.3839/jabc.2014.001
  8. Quality Characteristics and Optimization of Cookies Prepared with Opuntia humifusa Powder using Response Surface Methodology vol.29, pp.1, 2013, https://doi.org/10.9724/kfcs.2013.29.1.1
  9. Biological Activity of Phenol Compound from a Cactus Cheonnyuncho (Opuntia humifusa) in Korea vol.39, pp.8, 2010, https://doi.org/10.3746/jkfn.2010.39.8.1132
  10. Protective effects of Opuntia humifusa cladode extracts against biostir AD-induced atopic dermatitis in NC/Nga mice vol.24, pp.3, 2015, https://doi.org/10.1007/s10068-015-0143-7
  11. Inhibitory Effects of Allium senescens L. Methanol Extracts on Reactive Oxygen Species Production and Lipid Accumulation during Differentiation in 3T3-L1 Cells vol.46, pp.4, 2014, https://doi.org/10.9721/KJFST.2014.46.4.498
  12. The Effect of Alcohol Insoluble Polysaccharide (AIP) fromOpuntia Humifusaon Osteoporosis in Ovariectomized Mice vol.40, pp.6, 2016, https://doi.org/10.1111/jfbc.12261
  13. Bioactive Characteristics of Extracts of Opuntia humifusa Fruit as Functional Cosmetic Ingredients vol.14, pp.4, 2016, https://doi.org/10.20402/ajbc.2016.0080
  14. Inhibition of Adipocyte Differentiation and Adipogenesis by Supercritical Fluid Extracts and Marc from Cinnamomum verum vol.23, pp.4, 2013, https://doi.org/10.5352/JLS.2013.23.4.510
  15. Immune System-Stimulating Activities of Mucilage Polysaccharides Isolated from Opuntia humifusa vol.41, pp.1, 2012, https://doi.org/10.3746/jkfn.2012.41.1.095
  16. Food Components of Different Parts of Cheonnyuncho (Opuntia humifusa) Harvested from Yeosu, Jeonnam in Korea vol.40, pp.9, 2011, https://doi.org/10.3746/jkfn.2011.40.9.1271
  17. Anti-diabetic and Anti-oxidative Effects of Opuntia humifusa Cladodes vol.43, pp.5, 2014, https://doi.org/10.3746/jkfn.2014.43.5.661
  18. Physical and sensory characteristics of fibre-enriched sponge cakes made with Opuntia humifusa vol.47, pp.2, 2012, https://doi.org/10.1016/j.lwt.2012.02.011
  19. Antioxidant Effect of Hot water and Ethanol extracts from Cheonnyuncho (Opuntia humifusa) on Reactive Oxygen Species (ROS) Production in 3T3-L1 Adipocytes vol.19, pp.3, 2012, https://doi.org/10.11002/kjfp.2012.19.3.443
  20. Availability of Opuntia ficus-indica var. saboten Stem Extracts as a Natural Preservative vol.14, pp.4, 2016, https://doi.org/10.20402/ajbc.2016.0079
  21. The Effects of Antimicrobial, Antioxidant, and Anticancer Properties of Opuntia humifusa Stems vol.41, pp.1, 2012, https://doi.org/10.3746/jkfn.2012.41.1.020
  22. Antioxidative Effect of Suaeda japonica Ethanol Extract and Solvent Partitioned Fractions vol.40, pp.6, 2011, https://doi.org/10.3746/jkfn.2011.40.6.804
  23. Comparison of Biological Activities of Opuntia humifusa and Opuntia ficus-indica vol.26, pp.5, 2013, https://doi.org/10.7732/kjpr.2013.26.5.519
  24. Quality Characteristics during Storage of Rice Makgeolli Added with Cheonnyuncho Fermentative Extract vol.29, pp.6, 2013, https://doi.org/10.9724/kfcs.2013.29.6.679
  25. Suppressive Effect of Acorn (Quercus acutissima Carr.) Extracts in 3T3-L1 Preadipocytes vol.28, pp.4, 2015, https://doi.org/10.9799/ksfan.2015.28.4.650
  26. Diversity and antifungal activity of the endophytic fungi associated with the native medicinal cactus Opuntia humifusa (Cactaceae) from the United States vol.175, 2015, https://doi.org/10.1016/j.micres.2015.03.007
  27. Antiproliferative Effect of Opuntia humifusa Ethanol Extract on Human Carcinoma HT-29 Cells vol.43, pp.12, 2014, https://doi.org/10.3746/jkfn.2014.43.12.1827
  28. Antimicrobial Activity of Bamboo (Sasa borealis) Leaves Fraction Extracts against Food Poisoning Bacteria vol.39, pp.12, 2010, https://doi.org/10.3746/jkfn.2010.39.12.1745
  29. Opuntia humifusa Partitioned Extracts Inhibit the Growth of U87MG Human Glioblastoma Cells vol.65, pp.3, 2010, https://doi.org/10.1007/s11130-010-0188-y
  30. Physicochemical Characteristics of Cheonnyuncho Fruit (Opuntia humifusa) Fermented by Leuconostoc mesenteroides SM vol.45, pp.4, 2013, https://doi.org/10.9721/KJFST.2013.45.4.434
  31. Antioxidaive Activity of Lotus Root(Nelumbo nucifera G.) Extracts. vol.17, pp.9, 2007, https://doi.org/10.5352/JLS.2007.17.9.1237
  32. An Analysis of Design Factors for Developing Opuntia Humifusa Spines Removal Device vol.38, pp.3, 2013, https://doi.org/10.5307/JBE.2013.38.3.215
  33. Antioxidative Effect of Pimpinella brachycarpa Ethanol Extract. vol.18, pp.4, 2008, https://doi.org/10.5352/JLS.2008.18.4.467
  34. The Antioxidative Characteristics of Opuntia humifusa and its Optimal Conditions for Pasta Production vol.27, pp.6, 2012, https://doi.org/10.7318/KJFC/2012.27.6.710
  35. Antioxidants and Anti-obesity Activities of Hot Water and Ethanolic Extracts from Cheonnyuncho (Opuntia humifusa) vol.18, pp.3, 2011, https://doi.org/10.11002/kjfp.2011.18.3.366
  36. Antioxidant and anti-inflammatory activities of Opuntia ficus-indica and Opuntia humifusa fruits ethanol extracts vol.25, pp.5, 2018, https://doi.org/10.11002/kjfp.2018.25.5.586
  37. 허브추출물의 항균 작용 vol.34, pp.8, 2004, https://doi.org/10.3746/jkfn.2005.34.8.1130
  38. 연잎 에탄올 추출물의 항산화 효과 vol.35, pp.2, 2004, https://doi.org/10.3746/jkfn.2006.35.2.182
  39. 연잎 추출물의 항균 효과 vol.35, pp.2, 2006, https://doi.org/10.3746/jkfn.2006.35.2.219
  40. 천년초 추출물과 식이유황의 첨가가 계란의 생산성, 품질 및 관능적 특성에 미치는 영향 vol.39, pp.2, 2004, https://doi.org/10.3746/jkfn.2010.39.2.294
  41. 고지방식이에 의한 비만 동물모델에서 유산균 마분말의 항비만 효과 vol.29, pp.3, 2015, https://doi.org/10.15188/kjopp.2015.06.29.3.267
  42. 천년초의 융합발효를 통한 특성 연구 vol.8, pp.1, 2004, https://doi.org/10.15207/jkcs.2017.8.1.107
  43. Effect on the Emulsification Stability and Quality of Emulsified Sausages Added with Wanggasi-Chunnyuncho (Opuntia humifusa f. jeollaensis) Fruit Powders vol.39, pp.6, 2004, https://doi.org/10.5851/kosfa.2019.e85
  44. 제주도 손바닥선인장 추출물의 표면소독제로써의 항균효과 vol.23, pp.1, 2021, https://doi.org/10.7586/jkbns.2021.23.1.1