The Korean Journal of Applied Statistics (응용통계연구)

The Korean Statistical Society (한국통계학회)
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Statistical Analysis of a Loop Designed Microarray Experiment Data

되돌림설계를 이용한 마이크로어레이 실험 자료의 분석

  • 이선호 (세종대학교 응용수학과)
  • Published : 2004.11.01
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Abstract

Since cDNA microarray experiments can monitor expression levels for thousands of genes simultaneously, the experimental designs and their analyzing methods are very important for successful analysis of microarray data. The loop design is discussed for selecting differentially expressed genes among several treatments and the analysis of variance method is introduced to normalize microarray data and provide estimates of the interesting quantities. MA-ANOVA is used to illustrate this method on a recently collected loop designed microarray data at Cancer Metastasis Research Center, Yonsei University.

마이크로어레이 기술은 한번에 수만 개의 유전자를 동시에 분석할 수 있는 고효율, 고가의 새로운 연구 도구로 자리잡았으며 마이크로어레이 실험 자료의 올바른 분석을 위해서는 실험 목적에 맞는 실험계획법의 확립과 통계분석법의 적용이 중요하다 본 논문에서는 마이크로어레이 자료에서 여러 군 사이에서 발현의 차이를 보이는 유전자를 찾을 수 있는 되돌림 설계를 소개하고 ANOVA 모형을 이용하여 분석하는 방법을 제시한다. 연세대학교 암전이 연구센터의 되돌림 설계를 이용한 백혈병 자료를 MA-ANOVA(Wu et. al.(2003))를 이용하여 분석하였다

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References

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