Analysis of Diapause-Associated Changes in Silkworm Egg Proteins

  • Go, Hyun-Jeong (Department of Life Science, College of Natural Science, Suwon University) ;
  • Hwang, Jae-Sam (Department of Agricultural Biology, The National Institute of Agricultural Science and Technology) ;
  • Kim, Young-Tae (Department of Agricultural Biotechnology, The National Institute of Agricultural Science and Technology) ;
  • Kim, Hyun-Su (Department of Life Science, College of Natural Science, Suwon University) ;
  • Kang, Seok-Woo (Department of Agricultural Biology, The National Institute of Agricultural Science and Technology) ;
  • Chang, Jong-Su (Department of Life Science, College of Natural Science, Daejin University) ;
  • Lee, Sang-Mong (Department of Sericultural and Entomological Biology, Faculty of Agriculture, Miryang National University) ;
  • Lee, Bong-Hee (Graduate School of Biotechnology, Korea University) ;
  • Seong, Su-Il (Department of Life Science, College of Natural Science, Suwon University)
  • 발행 : 2004.09.01

초록

The patterns of diapause-associated proteins of silkworm eggs were analyzed by two-dimensional (2-D) gel electrophoresis. Among the hundreds of spots on the 2-D gels, at least two proteins were considered to be associated with diapause. A protein, spot 4, with an approximate molecular weight of 38 kDa and pI 6.1 was observed in the HCI-treated, cold-treated, and diapause eggs, respectively. Spot 4 was undetectable in unfertilized eggs and non-diapause eggs at two days after oviposition, suggesting that this protein may be associated with the entrance to diapause. A protein, spot 11, with an approximate molecular weight of 21 kDa and pI of 61 was detected in the unfertilized, HCl-treated, and cold-treated eggs, respectively, after oviposition by normal moths. In diapausing eggs, a protein corresponding to spot 11 was observed in 3-, 5-, and 30-day-old eggs, while the protein was not detected one-day-old eggs. The protein corresponding to spot 11 was not detected in unfertilized and non-diapause eggs obtained from subesophabeal ganglion (SG)-extirpated moths either. Spot 11 was also considered to be a diapause specific protein, which occurred at only early embryonic stage under the control of diapause-downregulated gene.

키워드

참고문헌

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