Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Purification and Biochemical Analysis of Rice Bran Lipase Enzyme

  • Kim, Young Hee (Dept. of Bioinformatics Engineering, College of Engineering, Sangmyung University)
  • Published : 2004.03.01
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Abstract

A simple procedure for the extraction of the lipolytic enzyme from rice bran has been developed. High activity of lipolytic enzyme was obtained by first defatting the rice bran to remove lipid components with various extraction conditions. Then, after rove cycles of aqueous extraction, rice bran lipolytic enzyme was purified using micro- and ultrafiltration apparatus. Lipolytic enzyme activity was estimated by its hydrolytic action of tributyrin. The result indicated that the standard activity curve of butyric acid showed that the potential rice bran enzyme is a hydrolytic lipase enzyme. In addition, it showed higher lipolytic activity and specific enzyme activity with further purification by micro- and ultrafiltration. The size of rice bran lipase enzyme was identified through 15 ％ SDS-PAGE. The molecular weight of the rice bran lipase enzyme was 41 kDa.

Keywords

References

  1. Aizono Y, Funatsu M, Sugano M, Hayashi K, Fujiki Y (1973) Enzymatic properties of rice bran lipase. Agric Biol Chem 37:2031-2036
  2. Amold RG, Shahani KM, Dwivedi BK (1975) Application of lipolytic enzymes to flavor development in dairy products. J Dair Sci 58:1127-1143
  3. Colburn JT (1969) Lipase-treated milk fats in production of butter/margarine flavors. United States Patent 3:477-857
  4. Coursey CG (1963) The deterioration of palm oil during storage, J West Afr Sci Assoc 7:101-114
  5. Desassis A (1957) Lacidification de I'huille de palme Oleagineux, 12:525-534
  6. Funatsu M, Aizono Y, Hayashi K, Watanabe M (1971) Biochemical studies on rice bran lipase I. Agric Biol Chem 35:734-742
  7. Gerhartz W (1990) Industrial uses of enzymes. In enzymes in industry-production and apptication, VCH, Weinhein, pp 77-148
  8. Godfrey T, Reichelt J (1983) Industrial application. In industrial enzymology-applications of enzymes in industry, The Nature Press, London, pp 170-465
  9. Hartley CWS (1977) The Oil Palm, 2nd ed. London: Longman Transesterification reaction. J Am Oil Chem Soc 59:471-474
  10. Kinsella JE, Hwang, DH, (1976) Enzymes of Penicillium roqueforti involved in the biosynthesis of cheese flavors. Crit Rev Food Sci 8:192-228
  11. Noda M, Kobayashi K (1968) Lipids of leaves and seeds IIIenzymic hydrolysis of rice bran lipids. Nippon Nogei Kagaku kaishi 42:574-580
  12. Prabhu AV, Tambe SP, Gandhi NN, Sawant SB, Joshi JB (1999)Rice bran lipases: extraction, activity, and stability. Biotechnol Prog 15:1083-1089
  13. Saphir J (1967) Permanent hair waving, West Germany Patentpp 1,242,794
  14. Shastry BS, Rao MRR (1971) Chemical studies on rice bran lipase. Cereal Chem 53:190-200
  15. Sood VK, Kosikowski FV (1979) Accelerated cheddar cheese ripening by added microbial enzymes. J Dairy Sci 62:1865-1872
  16. Stirton AJ (1964) Fat splitting, esterification and interesterification.,In Bailey's industrial oil and fat products, Bailey, A. E., Swern, D., Eds., John Wiley and Sons' New York, pp 937-972