Journal of Nutrition and Health

The Korean Nutrition Society (한국영양학회)
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Effect of Dietary Conjugated Linoleic Acid (CLA) Isomers on Tumor Incidence and the Protein Expression of Cyclooxygenase-2 and Protein Kinase C in Colonae Mucosa of DMH-Treated Rats

식이의 Conjugated Linoleic Acid (CLA) Isomer가 DMH로 처리한 쥐에서 대장점막의 종양발생과 Cyclooxygenase-2 및 Protein Linase C 단백질 발현에 미치는 영향

  • 박현서 (경희대학교 생활과학대학 식품영양학과) ;
  • 전창수 (경희대학교 생활과학대학 식품영양학과) ;
  • 윤정한 (한림대학교 생명과학부)
  • Published : 2004.11.01
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Abstract

This study was designed to compare the anti-carcinogenic effect of conjugated linoleic acid isomers on tumor incidence, cell proliferation and the levels of thromboxane (TX) B$_2$, prostaglandin (PG) E$_2$ and 1,2-diacylglycerol (DAG), and the related enzyme expression of cyclooxygenase (COX)-2 and protein kinase C (PKC) in colonic mucosa of 1,2-dimethy- lhydrazine (DMH) -treated rats. One hundred eight male Sprague Dawley rats were randomly divided into 3 groups depending on the types of CLA isomers, i.e. control group (no CLA contained), c9t11 group (cis-9, trans-11 CLA contained), and t10c12 group (trans-10, cis-12 CLA contained). The experimental diet was composed of protein at 20%, carbohydrate at 56.2%, and fat at 14.5% including 1.0% CLA isomers by weight. The experimental diet was fed for 30 weeks with the initiation of intramuscular injection of DMH, which was injected twice a week for 6 weeks to give total dose of 180 mg per kg body weight. Two CLA isomers (c9, t11, t10, c12) significantly reduced tumor incidence and cell proliferation by reducing the protein expression of COX-2 and PKC, and the level of TXB$_2$, PGE$_2$, and DAG in colonic mucosa. However, there was no significant difference in anti-carcinogenic effect between c9t11-CLA and t10c12-CLA.

Keywords

References

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