Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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DPPH Radical Scavenging Effect and Antimicrobial Activities of Plantain (Plantago asiatica L.) Extracts

질경이 추출물의 DPPH 라디칼 소거효과 및 항균활성

  • 정창호 (진주산업 대학교 식품과학과) ;
  • 배영일 (진주산업 대학교 식품과학과) ;
  • 심기환 (경상대학교 응용생명과학부) ;
  • 최진상 (진주산업 대학교 식품과학과)
  • Published : 2004.12.01
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Abstract

To develop the functional food, methanol extracts of dried plantain (Plantago asiatica L.) was fractionated in hexane, chloroform, ethyl acetate, butanol and aqueous fraction. DPPH radical scavenging effect, reducing power, nitrite scavenging effect and antimicrobial activities of these fractions were investigated. Ethyl acetate fraction among these fractions showed the highest effect of all experiments. Ethyl acetate fraction showed about 60% scavenging effect on the DPPH radicals at 400 $\mu$g/5 mL. Nitrite scavenging effect of ethyl acetate and butanol fraction was more than 70~80% at concentration above of 500 $\mu$g. Among various solvent fractions from methanol extracts of plantain, ethyl acetate fraction showed the highest inhibitory effect on the microorganism such as Staphylococcus aureus, Bacillus cereus and Vibrio parahaemolyticus at 5 mg/disc.

질경이를 새로운 기능성 식품소재로 활용하기 위한 일환으로 차전초의 메탄을 추출물을 각종 용매를 이용하여 계통$.$분획한 후 DPPH 라디칼 소거효과, 환원력, 아질산염 소거효과 및 항균활성에 대하여 조사하였다 DPPH 라디칼 소거효과는 분획물 중 에틸아세테이트와 부탄을 분획물(400 $\mu$g/5 mL)에서 60% 이상 높은 라디칼 소거율과 금속에 대한 강한 환원력을 나타낸 반면, 물, 클로로포름 및 헥산 분획물은 낮은 소거효과를 나타내었다. 질경이의 각 분획물을 농도별로 첨가한 아질산염 소거효과는 에틸아세테이트와 부탄올 분획물 500 $\mu$g 첨 가시 70∼80% 이상 높은 아질산염 소거효과를 나타내었다. 항균활성은 에틸아세테이트, 클로로포름 및 부탄을 분획물 순으로 높게 나타났으며, 에틸아세테이트 분획물의 경우 Staphylococcus aureus, Bacillus cereus 및 Vibrio parahaemolyticus 등의 그람양성 및 음성균 모두 강한 항균효과를 나타내었다.

Keywords

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