Archives of Pharmacal Research

  • 제27권10호
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  • Pages.1073-1079
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  • 2004
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  • 0253-6269(pISSN)
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  • 1976-3786(eISSN)
대한약학회 (The Pharmaceutical Society of Korea)
권호 표지

Vitamin C Blocks TNF-${\alpha}$-induced NF-kB Activation and ICAM-1 Expression in Human Neuroblastoma Cells

  • Son, Eun-Wha (Dept. of Pharmacognosy Material Development, Samcheok National University) ;
  • Mo, Sung-Ji (Division of Immunopharmacology, College of Pharmacy, Sungkyunkwan University) ;
  • Rhee, Dong-Kwon (Division of Immunopharmacology, College of Pharmacy, Sungkyunkwan University) ;
  • Pyo, Suhk-Neung (Division of Immunopharmacology, College of Pharmacy, Sungkyunkwan University)
  • 발행 : 2004.01.01
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초록

Interactions of the cell adhesion molecules are known to play important roles in mediating inflammation. The proinflammatory cytokine, tumor necrosis factor-${\alpha}$(TNF-${\alpha}$), activates the NF-kB signaling pathway, which induces the expression of various genes, such as intercellular adhesion molecule-1 (ICAM-1). In this study, the effect of vitamin C on the ICAM-1 expression induced by TNF-${\alpha}$ in a human neuroblastoma cell line, SK-N-SH was investigated. Treatment with vitamin C resulted in the downregulation of the TNF-${\alpha}$-induced surface expression and ICAM-1 mRNA levels in a concentration-dependent manner. Moreover, a gel shift analysis indicated that vitamin C dose-dependently inhibited the NF-kB activation and IkB${\alpha}$ degradation induced by TNF-${\alpha}$. Taken together, these results suggest that vitamin C downregulates TNF-${\alpha}$- induced ICAM-1 expression via the inhibition of NF-kB activation.

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