Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Determination of Betaine from Saliconia herbacea L.

함초(Saliconia herbacea L.)로부터 베타인 정량

  • Published : 2004.11.01
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Abstract

The betaine content of Saliconia herbacea L. was determined by reverse-phase high performance chromatog-raphy on a C_(18) column. A 50% methanol extract was passed through a anion exchanger Ambelite IRA 400 (quaternary ammonium type, OH-) column and a strong cation exchanger Ambelite IR 120 (sulfonic acid type, $H^+$) column to remove amino acids, zwitter ions which are interfere with betaine analysis. The betaine extract was derivatized with 18-crown-6-ether and 4-bromophenacyl bromide (PBPB) for UV-labelling. Betaine in Saliconia herbacea L. was analysed on a mobile phase contained 13 mM sodium heptane sulfonic acid and 5 mM $Na_2SO_4$ in deionized water by isocratic elution for 30 min. The recovery ratio of betaine from Saliconia herbacea L. extract was 83.6%. The mean betaine value for Saliconia herbacea L. determined by the described method is 4.85 mg/mL with a standard deviation of 0.127.

해안가 및 폐염전 등에 서식하며 각종 미네랄, 아미노산 및 유용 생리활성 물질을 함유하고 있는 함초로부터 혈중 동맥경화 및 심장 질환 예방에 유효한 효능을 보일 것으로 예상되는 성분인 betaine을 HPLC를 이용하여 분석하였다. 함초는 양쪽성 물질로 같이 존재하는 다른 이온성 물질들에 의해 방해를 받으며 UV 흡수가 매우 낮아 일반적인 정량방법으로는 분석이 매우 까다롭다. 따라서 본 연구에서는 이러한 문제를 해소하기 위하여 2단계 이온교환수지 컬럼을 이용하여 방해물질을 제거한 후 betaine을 4-bromophenacyl bromide (PBPB)로 유도체화시켜 UV-labelling 한 후 분석하였다. 이온교환수지를 통과한 함초추출물의 회수율은 83.6%이며 함초추출액 mL당 4.85mg의 betaine이 함유되어 있는 것으로 나타났다.

Keywords

References

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