Mycobiology

  • 제32권4호
  • /
  • Pages.170-172
  • /
  • 2004
  • /
  • 1229-8093(pISSN)
  • /
  • 2092-9323(eISSN)
한국균학회 (The Korean Society of Mycology)
권호 표지
DOI QR코드

DOI QR Code

Effect of Different Pretreatment Methods on the Bioconversion of Rice Bran into Ethanol

  • Eyini, M. (Research Centre in Botany, Thiagarajar College(Autonomous)) ;
  • Rajapandy, V. (Research Centre in Botany, Thiagarajar College(Autonomous)) ;
  • Parani, K. (Research Centre in Botany, Thiagarajar College(Autonomous)) ;
  • Lee, Min-Woong (Department of Biology, Dongguk University)
  • 발행 : 2004.12.31
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

The efficiency of acid, enzyme and microbial pretreatment of rice bran was compared based on the content of cellulose, hemicellulose, reducing sugars and xylose in the substrate. An isolate of Aspergillus niger or a strain of Trichoderma viride(MTCC 800) was employed for microbial pretreatment of rice bran in solid state. Acid pretreatment resulted in the highest amount of reducing sugars followed by enzyme and microbial pretreatment. A. niger showed a higher rate of hydrolysis than T. viride. The rice bran hydrolysate obtained from the different methods was subsequently fermented to ethanol either by Zymomonas mobilis(NCIM 806) or by Pichia stipitis(NCIM 3497). P. stipitis fermentation resulted in higher ethanol(37% higher) and biomass production($76{\sim}83%$ higher) than those of Z. mobilis. Maximum ethanol production resulted at 12h in Zymomonas fermentation, while in Pichia fermentation, it was observed at 60h. Microbial pretreatment of rice bran by A. niger followed by fermentation employing P. stipitis was more efficient but slower than the other microbial pretreatment and fermentation.

키워드

참고문헌

  1. Caputi, A., Veda, M. and Brown, T. 1968. Spectrophotometric determination of ethanol in wine. Amer. J. Enol. Viticul. 19: 160-165
  2. Doelle, H. W. M. and Greenfield, P. F. 1985. The production of ethanol from sucrose using Zymomonas mobilis. Appl. Microbiol. Biotechnol. 22: 405-410
  3. Dupreez, J. C., Bosch, M. and Prior, B. A. 1986. The fermentation of hexose and pentose sugars by Candida shehate and Pichia stipitis. Appl. Microbiol. Technol. 23: 228-233 https://doi.org/10.1007/BF00261920
  4. Hinman, N. D., Wright, J. D., Hoagland, W. and Wyman, C. E. 1989. Xylose fermentation - an economic analysis. Appl. Biochem. Biotechnol. 20/21: 391-401 https://doi.org/10.1007/BF02936498
  5. Kim, C. H., Lee, G. M., Zainal, A, Han., M. H. and Rhee, S. K. 1988. Immobilization of Zymomonas mobilis and amyloglucosidase for ethanol production from sago starch. Enzyme Microb. Technol. 10: 416
  6. Miller, G. L. 1959. Use of DNS reagent for the determination of reducing sugars. Anal. Chem. 31: 426-428 https://doi.org/10.1021/ac60147a030
  7. Ray, L., Pal, A, Ghosh, A. K. and Chattopadhyay, P. 1993. Cellulases and $\beta$-glucosidase from Aspergillus niger and saccharification of some cellulosic wastes. J. Microbial Biotechnol. 8: 85-94
  8. Reddy, B. R., Narasimha, G. and Babu, G. V. A. K. 1998. Cellulolyticactivity of fungal cultures. J. Sci. Ind. Res. 57: 617-620
  9. Rhee, S. K., Lee, G. M., Kim, C. H., Abidin, Z. and Han, M. H. 1986. Simultaneous sago starch hydrolysis and ethanol production by Zymomonas mobilis and glucoamylase. Biotechnol. Bioeng. Symp. 17: 481
  10. Schele, D. J., Torget, R, Power, A., Walter, P. J., Grohmann, K. and Hinman, N. D. 1991. A technical and economic analysis of acid catalysed steam explosion and dilute sulphuric acid pretreatment using wheat straw or aspen wood chips. Appl. Biochern. Biotechnol. 28/29: 87-98 https://doi.org/10.1007/BF02922591
  11. Torget, R., Walter, P. J., Himmel, M. and Grohmann, K. 1991. Dilute acid pretreatment of corn residues and short rotation woody crops. Appl. Biochem. Biotechnol. 28/29: 75-86