Imaging Science in Dentistry

Korean Academy of Oral and Maxillofacial Radiology (대한영상치의학회)
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Effects of irradiation on the mRNA expression of osteonectin and bone sialoprotein in MC3T3-E1 osteoblastic cell line

MC3T3-E1 조골세포주의 osteonectin과 bone sialoprotein mRNA

  • Ha Ssang-Yong (Department of Oral and Maxillofacial Radiology, School of Dentistry, and Institute of Oral Bio Science, Chonbuk National University) ;
  • Kang Ki-Hyun (Department of Oral and Maxillofacial Radiology, School of Dentistry, and Institute of Oral Bio Science, Chonbuk National University) ;
  • Lee Sang-Rae (Department of Oral and Maxillofacial Radiology, College of Dentistry, Kyung Hee University) ;
  • Kwon Ki-Jeong (Department of Oral and Maxillofacial Radiology, School of Dentistry, and Institute of Oral Bio Science, Chonbuk National University) ;
  • Koh Kwang-Joon (Department of Oral and Maxillofacial Radiology, School of Dentistry, and Institute of Oral Bio Science, Chonbuk National University)
  • 하쌍용 (전북대학교 치과대학 구강악안면방사선학교실 및 구강생체과학연구소) ;
  • 강기현 (전북대학교 치과대학 구강악안면방사선학교실 및 구강생체과학연구소) ;
  • 이상래 (경희대학교 구강악안면방사선학교실) ;
  • 권기정 (전북대학교 치과대학 구강악안면방사선학교실 및 구강생체과학연구소) ;
  • 고광준 (전북대학교 치과대학 구강악안면방사선학교실 및 구강생체과학연구소)
  • Published : 2004.06.01
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Abstract

Purpose: To investigate the effects of irradiation on the phenotypic expression of the MC3T3-El osteoblastic cell line, especially on the osteonectin and bone sialoprotein. Materials and Methods: Cells were irradiated with a single dose of 0.5, 1, 4 and 8 Gy at a dose rate of 5.38 Gy/min using Cs-I37 irradiator. After specimens were harvested, total RNA was extracted on the 3rd, 7th, 14th, 21st day after irradiation. The total RNA was reverse-transcribed and the resulting cDNAs were subjected to amplification by PCR with a pair of primers. Results: The irradiated cells showed a dose-dependent increase in osteonectin mRNA expression when compared with the unirradiated control group. The irradiated cells showed no difference in bone sialoprotein mRNA expression when compared with the unirradiated control group. In accordance with the duration of culture period after irradiation, the level of osteonectin mRNA expression showed no difference, but it increased a little at the 21st day in the 4 and 8 Gy exposure groups. In the case of bone sialoprotein, however, the level of mRNA expression increased significantly at the 3rd and 7th day after irradiation, but it showed no difference at the 14th and 21st day when compared with the control group. Conclusion: These results showed that each single dose of 0.5, 1, 4 and 8 Gy influenced the mRNA expression of osteonectin and bone sialoprotein at the calcification stage of osteoblastic cells, suggesting that single dose of irradiation affected the osteoblastic bone formation at the cell level.

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