The Journal of Korean Medicine (대한한의학회지)

The Society of Korean Medicine (대한한의학회)
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Study on the Effect of Gamisihotang(GSHT) on Glutamate Receptor, Free Radical and Brain Damage in Rats Subjected to Brain Ischemia

가미시호탕이 뇌허혈시 Glutamate receptor, free radical 및 뇌손상 보호에 미치는 영향

  • Oh Byung-Yul (Dept. of Internal Medicine, College of Oriental Medicine, DaeJeon University) ;
  • Kim Min-Sang (Dept. of Internal Medicine, College of Oriental Medicine, DaeJeon University) ;
  • Yu Byeong-Chan (Dept. of Internal Medicine, College of Oriental Medicine, DaeJeon University) ;
  • Choi Young (Dept. of Internal Medicine, College of Oriental Medicine, DaeJeon University) ;
  • Seol In-Chan (Dept. of Internal Medicine, College of Oriental Medicine, DaeJeon University)
  • 오병열 (대전대학교 한의과대학 내과학교실) ;
  • 김민상 (대전대학교 한의과대학 내과학교실) ;
  • 유병찬 (대전대학교 한의과대학 내과학교실) ;
  • 최영 (대전대학교 한의과대학 내과학교실) ;
  • 설인찬 (대전대학교 한의과대학 내과학교실)
  • Published : 2004.09.01
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Abstract

Objectives : This study was undertaken to prove the effect of GSHT on the glutamate receptor, free radical and brain damage in rats subjected to brain ischemia Methods : Levels of cultured cortical neuron death caused by toxic chemicals were measured by LDH release assay. Neuroprotective effects of GSHT on brain tissues were examined in vivo by ischemic model of middle cerebral artery (MCA) occlusion. Results : GSHT showed significant inhibitory effect on LDH release induced by NMDA-kinate-Fe/sup 2+/. GSHT remarkably decreased coma duration time in a nonfatal dose of KCN and showed higher survival rate in a fatal dose. GSHT remarkably decreased ischemic area and edema induced by the MCA blood flow block. GSHT showed high improvement of forelimb and hind limb test after MCA occlusion in neurological examination. GSHT showed no significant change after MCA occlusion in pathological observation of the normal group. Conclusions : These results indicate that GSHT can be used to treat the brain damage caused by brain ischemia. Further study will be needed about the functional mechanism, etc.

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