Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
권호 표지

Inhibitory Effect of Retinoic Acid on lipid Synthesis in Human Sebocyte

피지선세포에서 Retinoic Acid의 피지생성억제효과

  • Mun Yeun Ja (Department of Herbal Resources, Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Kim Youn Seok (Department of Herbal Resources, Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Kwon Gang Joo (Department of Herbal Resources, Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Rhee Hee Sub (Department of Obstetrics and Gynecology, Missbebe Hospital) ;
  • Roh Seong Taek (Department of Herbal Resources, Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Kim Yang Jin (Department of Herbology & Prescriptionology, Oriental Medicine College, Sangji University) ;
  • Lee Jang Cheon (Department of Herbology & Prescriptionology, Oriental Medicine College, Sangji University) ;
  • Woo Won Hong (Department of Herbal Resources, Professional Graduate School of Oriental Medicine, Wonkwang University)
  • 문연자 (원광대학교 한의학전문대학원 한약자원개발학과) ;
  • 김윤석 (원광대학교 한의학전문대학원 한약자원개발학과) ;
  • 권강주 (원광대학교 한의학전문대학원 한약자원개발학과) ;
  • 이희섭 (미즈베베병원 산부인과) ;
  • 노성택 (원광대학교 한의학전문대학원 한약자원개발학과) ;
  • 김양진 (상지대학교 한의과대학 본초방제학교실) ;
  • 이장천 (상지대학교 한의과대학 본초방제학교실) ;
  • 우원홍 (원광대학교 한의학전문대학원 한약자원개발학과)
  • Published : 2004.10.01
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Abstract

The differentiation of the sebaceous gland is remarkably species-specific and sebocytes may play crucial parts in the pathophysiologic processes and disorders of pilosebaceous unit SZ95 cell is an immortalized human sebaceous gland cell line that shows characteristics of normal human sebocytes, In this study, we investigated the effect of testosterone and the anti-androgenic effect of 13-cis-retinoic acid (13-cis-RA) on lipid synthesis in SZ95 cells. Cytoplasmic lipid droplets were shown by Oil-red staining. The majority of the SZ95 cells positively labeled with Oil-red dye, while HaCaT cells negatively labeled with Oil red dye. Total lipid level of SZ96 cells is higher 4 times than that of HaCat cells. Testosterone markedly increased 2 times lipid synthesis of SZ95 cells in compared with control. 13-cis-RA significantly inhibited lipid synthesis and cell proliferation in SZ95 cells. Combined treatment with testosterone and 13-cis-RA resulted in a lower total lipid levels than that with androgen alone. In conclusion, SZ95 cells well resembled the morphologic and functional characteritics of normal human sebocytes. This in vitro model could provide a valuable tool for the study of sebocytes with a key role in pathophysiology and differentiation of sebaceous glands.

Keywords

References

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