Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
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The Effects of Anti-Alzheimer on CT105-induced PC 12 Cells by Corynoxeine Isolated from Uncariae Ramulus et Uncus

pCT105로 원격 유도된 PC12 세포에서 조구등으로부터 분리한 corynoxeine의 항치매 효과

  • Kang Hyung Won (Department of Oriental Neuropsychiatry Medicine, College of Oriental Medicine, Wonkwang University) ;
  • Kim Sang Tae (JuAm of Research Institute Bioscience & Biotechnology) ;
  • Lyu Yeoung Su (Department of Oriental Neuropsychiatry Medicine, College of Oriental Medicine, Wonkwang University)
  • 강형원 (원광대학교 한의과대학 한방신경정신과학교실) ;
  • 김상태 (주암생명공학연구소) ;
  • 류영수 (원광대학교 한의과대학 한방신경정신과학교실)
  • Published : 2004.08.01
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Abstract

In this study, we investigated that the effects of corynoxeine on the apoptosis by inducible CT105 in PC 12 cells and neuronpathogenic agent as CT105 confirmed with apoptosis, DNA fragmentation, neurite outgrowth and immunocytochemistry analysis This study examines whether corynoxeine have an anti-alzhmeimer agent by inhibition of apoptosis by CT105 and induces neurite outgrowth. Cytotoxicity was assessed in PC12 cell cultures by DNA fragmentation and measuring lactate dehydrogenase (LDH) in the culture media. The treatment of corynoxeine in exposure of cultures to CT105 and provided complete protection against cytotoxicity. CT105-induced cytotoxicity was blocked by apoptotsis, repaired by DNA fragmentation, neurite outgrowth and exposure to CT105 expression and regenerated with neurite outgrowth and immunocytochemistry by corynoxeine. These results indicate that in neuronal cell cultures, damage of T105, repaired excitotoxicity by corynoxeine and CT105-induced cytotoxicity is blocked primarily by the activation of anti-apoptosis.

Keywords

References

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