Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
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Study on the Effect of Gamissanghwa-tang and each Medicinal Plant Extract for the Hair Growth of the Mice using In vivo and In vitro Test

In vivo와 In vitro 실험에서 가미쌍화탕 및 구성한약재가 마우스의 모발 성장에 미치는 실험적 연구

  • Yun Jeong Hun (Department of Ophthalmology Otolaryngology and Dermatology, College of Oriental Medicine, Wonkwang University) ;
  • Kim Nam Kwen (Department of Ophthalmology Otolaryngology and Dermatology, College of Oriental Medicine, Wonkwang University) ;
  • Lim Kyu Sang (Department of Ophthalmology Otolaryngology and Dermatology, College of Oriental Medicine, Wonkwang University) ;
  • Roh Seok Seon (Department of Ophthalmology Otolaryngology and Dermatology, College of Oriental Medicine, Daejeon University) ;
  • Hwang Chung Yeon (Department of Ophthalmology Otolaryngology and Dermatology, College of Oriental Medicine, Wonkwang University)
  • 윤정훈 (원광대학교 한의과대학 인이비인후피부과교실) ;
  • 김남권 (원광대학교 한의과대학 인이비인후피부과교실) ;
  • 임규상 (원광대학교 한의과대학 인이비인후피부과교실) ;
  • 노석선 (대전대학교 한의과대학 안이비인후피부과교실) ;
  • 황충연 (원광대학교 한의과대학 인이비인후피부과교실)
  • Published : 2004.04.01
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Abstract

To screen the effective materials for hair loss treatment, the Gamissanghwa-tang extracts were tested. As a result we found that the Gamissanghwa-tang extracts have the hair growth promoting effect. After topical application of each test materials to the back of CS7BL/6 mice, the earlier conversion of telogen-to-anagen phase was induced. In the experiments of 5α-reductase type II inhibition assay, Radix Paeoniae Alba, Semen Cuscutae showed effective potential to inhibit the activity of 5α-reductase type II. And hair growth index of the Gamissanghwa-tang extracts ranked as 1.2, especially the hair growth index of Fructus Rubi is highest as 1.8. But there were no plant extracts which have effect on the DNA proliferation of hair dermal papilla cell measured by [³H]thymidine incorporation, the expression of growth factors such as IGF-I, KGF, HGF estimated by RT-PCR and protein synthesis of vibrissae hair follicle measured by [/sup 35/S] cysteine incorporation. Cortex Cinnamomi showed anti-bacterial effect on P. ovale, Radix Paeoniae Alba has the highest radical scavening activity and Radix Glycyrrhizae has the highest effects of NO synthesis. These results suggest that Gamissanghwa-tang can be used as a potent treatment agent for helping hair growth stimulation.

Keywords

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