Journal of the Society of Cosmetic Scientists of Korea (대한화장품학회지)

Society of Cosmetic Scientists of Korea (대한화장품학회)
권호 표지

Anti-melanogenesis Effect of Canavalia lineata Extract

해녀콩(Canavalia lineata THUNB. DC.) 추출물의 멜라닌 생성 억제 효과

  • Bu Hee-Jung (Department of Chemistry, Cheju National University) ;
  • Riu Key-Zung (Division of Horticultural Life Science) ;
  • Lee Sunjoo (Department of Chemistry, Cheju National University)
  • 부희정 (제주대학교 자연과학대학 화학과) ;
  • 류기중 (제주대학교 원예생명과학과) ;
  • 이선주 (제주대학교 자연과학대학 화학과)
  • Published : 2004.12.01
Download PDF
⟨ Previous Next ⟩

Abstract

Melanin pigmentation in human skin is a major defensive mechanism against ultraviolet light of the sun. Tyrosinase plays a key role in the biosynthesis of melanin. This is why many researches have been focused on regulations in controlling the epidermal melanization. We found that extract of Canavalia lineata inhibits mushroom tyrosinase activity, dopa oxidase activity, and melanin synthesis in B16F10 melanoma cells. To elucidate mRNA level reverse transcription polymerase chain reaction (RT-PCR) technique was used. It was revealed that A subfraction of $CHCI_3$ extract of Canavalia lineara reduced the tyrosinase mRNA expression of B16F10 melanoma cells by reverse transcription polymerase chain reaction (RT-PCR) technique.

피부에서 melanin은 자외선 차단의 주요한 역할을 한다. Tyrosinase는 멜라닌 생합성과정에서 초기 단계에 관여하는 중요한 효소로서 이것의 조절을 통한 피부 멜라닌화 억제에 관해 많은 연구가 되어져왔다. 본 연구에서는 해녀콩 추출물에서 mushroom tyrosinase 활성억제, B16F10 melanoma 세포를 이용한 dopa oxidase 활성억제 및 멜라닌 합성 억제 효과를 확인하였다. Tyrosinase mRNA 발현에서의 억제 효과를 확인하기 위하여 RT-PCR을 이용하였으며, $CHCI_3$ 층에서 분리해낸 A 분획에서 tyrosinase mRNA 발현을 억제시킴을 확인하였다.

Keywords

References

  1. Y. Mishima, S. Hatta, and Y. Ohyama, Induction of melanogenesis su-ppression: cellular pharmacology and mode of differential action, Pigment Cell Res., 1, 367 (1988)
  2. H. Matubara, Inhibitory effect of lichen metabolites and their synthetic analogues on melanin biosynthesis in cultured B-16 mouse melanoma cells, Natural Product Scienses, 4, 3 (1998)
  3. B. W. Choi, B. H. Lee, K. J. Kang, E. S. Lee, and N. H. Lee, Screening of the tyrosinase inhibitors from marine algae and medicinal plants, Kor. J. Pharmacogn., 29, 3 (1998)
  4. S. Pavel, Dynamics of melanogenesis intermediate, J. Invest Dermatol., 162 (1993)
  5. B. A. Gilcherst, S. Zhai, M. S. Eller, D. B. Yarosh, and R. M. Yaa, Treatment of human melanocytes and S91 melanoma cells with the DNA repair enzyme T4 endonuclease V enhances melanogenesis after ultraviolet irradiation, J. Invest. Dermatol., 101, 666 (1993)
  6. V. J. Hearing, Mammalian monophenol monooxygenase (tyrosinase): Purification, properties, and reaction catalyzed, Methods in Enzymo logy, 142, 154 (1987)
  7. T. Mosmann, Rapid colorimetric assay for the cellular growth and survival application to proliferation and cytotoxic assay, J. Immun. Methods, 65, 55 (1983)
  8. K. Meada and M. Fukuda, In vitro effectiveness of whitening cosmetic components in human melanocyte, J. Soc. Cosmet. Chem., 42, 361 (1991)
  9. P. R. Gordon, C. P. Mansur, and B. A. Gilchrest, Regulation of human melanocyte growth, dendricity and melanization by keratinicyte derived factors, J. Invest Dermatol., 2, 566 (1989)
  10. V. J. Hearing, Biochemical control of melanogenesis and melanosomal organization, Soc. Invest. Dermatol., 4(1), 24 (1999)
  11. M. Eisinger and O. Marko, Selective proliferation of normal human melanocytes in vitro in the presence of phorbol ester and cholera toxin, Proc. Natl. Acad. Sci. U.S.A., 79, 2018 (1982)