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Rapid Bioassay Technique Based on Temperature Control of Ceriodaphnia dubia

Ceriodaphnia dubia의 온도조절에 근거한 단기급성독성 조사법

  • PARK Jong Ho (Department of Inland Fisheries Research Institute, Chung Cheong Buk-Do) ;
  • LEE Sang Ill (Department of Environmental Engineering, Chung Buk National University) ;
  • CHO Young Oak (Department of Environmental Engineering, Chung Buk National University) ;
  • LEE Won Ho (Department of Construction & Urban Engineering, Chung Ju National University) ;
  • YEON Ik Jun (Department of Environmental Engineering, Chung Ju National University) ;
  • CHO Kyu Seok (Department of Inland Fisheries Research Institute)
  • Published : 2004.06.01

Abstract

A method for rapid acute toxicity test based on temperature control of Ceriodaphnia dubia has been developed and evaluated. A new toxicity test based on temperature control (TTBTC) which are based on temperature control, was developed and compared for the adsorption of the better methodology for short-term toxicity screening. Initially, daphnid larval are exposed to toxicants and at the same time the temperature of the water bath containing media is increased to high temperature $(35.5^{\circ}C).$ After 1.25 hrs of contact time, the number of the daphnids, either living (no toxic effect) or dead (toxic effect), is counted by the naked eyes. Effect of exposure time on test sensitivity was not significantly different between 1 to 1.5 hr. Comparison of the rapid 1.25 hr acute toxicity test developed in this study and the standard 48 hr acute toxicity test using heavy metals, cyanide and pentachlorophenol indicated that the 1.25 hour test provides an acceptable level of sensitivity in toxicity test for C. dubia.

Keywords

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