DOI QR코드

DOI QR Code

Relationship of Specific Microbial Growth and TBARS Value in Radiation-Sterilized Raw Ground Pork

  • Kim, Jae-Kyung (Radiation Food Science and Biotechnology Team, Korea Atomic Energy Research institute) ;
  • Jo, Cheo-Run (Radiation Food Science and Biotechnology Team, Korea Atomic Energy Research institute) ;
  • Kim, Hyun-Joo (Radiation Food Science and Biotechnology Team, Korea Atomic Energy Research institute) ;
  • Lee, Kyong-Haeng (Department of Kimchi & Food Science, Chongju National College of Science and Technology) ;
  • Kim, Yeung-Ji (Division of Food, Beverage & Culinary Art, Yeungnam College of Science & Technology) ;
  • Byun, Myung-Woo (Radiation Food Science and Biotechnology Team, Korea Atomic Energy Research institute)
  • Published : 2004.12.01

Abstract

Sterilized raw ground pork was inoculated with Pseudomonas aeruginosa (PA) and Lactobacillus casei (LC) to investigate the relationship between microbial growth and 2-thiobarbituric acid reactive substances (TBARS) values. The analyses including microbial growth, pH, and TBARS values were performed during 3 weeks of storage at room temperature $(20^{\circ}C)$. The radiation-sterilized control sample did not show any microbial growth, but the samples inoculated at different levels (diluted twice vs non-diluted) exhibited differences until 1 week. However, the difference disappeared at weeks 2 and 3. The pH of raw ground pork inoculated with PA increased, but that of LC decreased. The pH of non-inoculated samples increased slightly after storage. The TBARS values in non-inoculated and LC inoculated with pork increased, but TBARS remained unchanged in samples inoculated with PA after 1 week. Results indicated that the microbial growth level and strains can influence the TBARS value of raw ground pork. Thus, it is important to use samples exposed to the same microbial conditions to compare the oxidation of lipids in meat samples.

Keywords

References

  1. Tarladgis BG, Watts BM, Younathan MT, Dugan LR Jr. 1960. A distillation method for the quantitative determination of malonaldehyde in rancid flavor. J Am Oil Chem Soc 37: 44-48 https://doi.org/10.1007/BF02630824
  2. Jo C, Ahn DU. 1998. Fluorometic analysis of 2-thiobarbituric acid reactive substances in turkey. Poultry Sci 77: 475-480 https://doi.org/10.1093/ps/77.3.475
  3. Allen CE, Foegeding EA. 1981. Some lipid characteristics and interactions in muscle foods. A review. Food Technol 35: 253-257
  4. Salih AM, Smith DM, Prince JR, Dawson LE. 1987. Modified extraction 2-thiobarbituric acid method for measuring lipid oxidation in poultry. Poultry Sci 66: 1483-1488 https://doi.org/10.3382/ps.0661483
  5. Kakuda Y, Stanley DW, van de Voort FR. 1981. Determination of TBA number by high performance liquid chromatography. J Am Oil Chem Soc 58: 773-775 https://doi.org/10.1007/BF02887320
  6. Shlafer M, Shepard BM. 1984. A method to reduce interference by sucrose in the detection of thiobarbituric acid-reactive substances. Anal Biochem 137: 269-276 https://doi.org/10.1016/0003-2697(84)90084-8
  7. Smith JL, Alford JA. 1969. Action of microorganisms on the peroxidies and carbonyls of fresh fat. J Food Sci 56: 597-600
  8. Bothast RJ, Kelly RF, Graham PP. 1973. Influence of bacteria on the carbonyl compounds of ground porcine muscle. J Food Sci 38: 75-78 https://doi.org/10.1111/j.1365-2621.1973.tb02779.x
  9. Moerck KE, Ball HR Jr. 1974. Lipid autoixdation in mechanically deboned chicken meat. J Food Sci 39: 876-879 https://doi.org/10.1111/j.1365-2621.1974.tb07265.x
  10. Moerck KE, Ball HR Jr. 1979. Influence of microorganisms on the carbonyl compounds of chicken tissue. J Agric Food Chem 27: 854-859 https://doi.org/10.1021/jf60224a050
  11. Branen AL. 1978. Interaction of fat oxidation and microbial spoilage in muscle foods. Proceedings, 31st Reciprocal Meat Conference. National Live Stock and Meat Board. Chicago, IL. p 156-161
  12. Rhee KS, Krahl LM, Lucia LM, Acuff GR. 1997. Antioxidative/antimicrobial effects and TBARS in aerobically refrigerated beef as related to microbial growth. J Food Sci 62: 1205-1210 https://doi.org/10.1111/j.1365-2621.1997.tb12245.x
  13. Bala K, Marshall RT, Stringer WC, Naumann HD. 1977. Effect of Pseudomonas ftagi on the color of beef. J Food Sci 42: 1176-1179 https://doi.org/10.1111/j.1365-2621.1977.tb14454.x
  14. Lillard HS, Ang CYW. 1989. Relationship of microbiological quality and oxidative stability of raw broiler meat during cold storage. Poultry Sci 56: 220-223
  15. Nattingham PM. 1982. Microbiology of carcass meats. In Meat microbiology. 4th ed. Brown MH, ed. Applied Science Publishers Ltd., New York. p 43-56
  16. SAS Institute, Inc. 1990. SAS User's Guide. Statistical Analysis Systems Institute, Cary, NC, USA
  17. Lakshmanan R, Jeya Shakila R, Jeyasekaran G. 2002. Changes in the halophilic amine forming bacteria flora during salt-drying of sardines (Sardinella gibbosa). Food Res Int 35: 541-546 https://doi.org/10.1016/S0963-9969(01)00154-5
  18. Jay JM. 1992. Spoilage of fresh and processed meats, poultry, and seafood. In Modern Food Microbiology. 4th ed. Van Nostrand Reinhold, New York. Ch 9, p 199-233
  19. Lefebvre N, Thibault C, Charbonneau R, Piette JPG. 1994. Improvement of shelf-life and wholesomeness of ground beef by irradiation-2. Chemical analysis and sensory evaluation. Meat Sci 36: 371-380 https://doi.org/10.1016/0309-1740(94)90133-3
  20. Carlin F, Nguyen-the C, Cudennec P, Reich M. 1989. Microbiological spoilage of ready-to-use grated carrots. Sciences Des Aliments 9: 371-386
  21. Yoo IK, Chang HN, Lee EG, Chang YK, Moon SH. 1996. Effect of pH in the production of lactic acid and secondary products in batch cultures of Lactobacillus casei. J Microbiol Biotechnol 6: 482-486
  22. Minor-Prez H, Ponce-Alquicira E, Mac?as-Bravo S, Guerrero-Legarreta I. 2004. Changes in fatty acids and microbial populations of pork inoculated with two biopreservative strains. Meat Sci 66: 793-800 https://doi.org/10.1016/S0309-1740(03)00173-6
  23. Rhee KS. 1992. Fatty acids in meat and meat products. In Foods Acids in Foods and Their Health Implications. Chow CK, ed. Marcel Dekker, Inc, New York. Ch 4, p 5-93

Cited by

  1. Reduction of irradiation off-odor and lipid oxidation in ground beef by α-tocopherol addition and the use of a charcoal pack vol.78, pp.2, 2009, https://doi.org/10.1016/j.radphyschem.2008.09.003
  2. Antioxidant effects of lotus (Nelumbo nucifera) root and leaf extracts and their application on pork patties as inhibitors of lipid oxidation, alone and in combination vol.22, pp.1, 2004, https://doi.org/10.1080/10942912.2019.1588295
  3. Antioxidant effects of lotus (Nelumbo nucifera) root and leaf extracts and their application on pork patties as inhibitors of lipid oxidation, alone and in combination vol.22, pp.1, 2004, https://doi.org/10.1080/10942912.2019.1588295
  4. Storage Stability of Vacuum-packaged Dry-aged Beef during Refrigeration at 4℃ vol.39, pp.2, 2019, https://doi.org/10.5851/kosfa.2019.e21