Journal of Ginseng Research

The Korean Society of Ginseng (고려인삼학회)
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Effect of Phytagel on the Root Growth of Plantlets Redifferentiated from Ginseng(Panax ginseng) Embryogenic Cells

인삼 배발생세포에서 재분화된 유식물체의 뿌리 발육에 미치는 Phytagel의 영향

  • Ahn, In-Ok (Agro-Tech Research Group, KT&G Central Research Institute) ;
  • Choi, Young-Jun (Department of Horticultural Science, Yeungnam University) ;
  • Lee, Sung-Sik (Agro-Tech Research Group, KT&G Central Research Institute) ;
  • Lee, Jang-Ho (Agro-Tech Research Group, KT&G Central Research Institute) ;
  • Kang, Je-Yong (Agro-Tech Research Group, KT&G Central Research Institute) ;
  • Yu, Yun-Hyun (Agro-Tech Research Group, KT&G Central Research Institute) ;
  • Chang, Hee-Don (Department of Horticultural Science, Yeungnam University)
  • 안인옥 (KT&G 중앙연구원 원료연구소) ;
  • 최영준 (영남대학교 자연자원학부) ;
  • 이성식 (KT&G 중앙연구원 원료연구소) ;
  • 이장호 (KT&G 중앙연구원 원료연구소) ;
  • 강제용 (KT&G 중앙연구원 원료연구소) ;
  • 유연현 (KT&G 중앙연구원 원료연구소) ;
  • 정희돈 (영남대학교 자연자원학부)
  • Published : 2004.12.01
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Abstract

This study was carried out to find out the optimum hardening condition for ginseng plantlets redifferentiated by tissue culture method. While a lot of root hair were observed on the root of seedling grown on the soil, few root hair were observed on the root of plantlet redifferentiated in vitro. On the medium solidified with $0.1{\%}$ phytagel, root hair was not observed and root weight, root width and laternal root development were also very poor. While raising the phytagel concentration on the media, root hair began to increase and root weight, root width and latemal root development were improved. Vascular tissue of plantlet grown on the medium with $0.1{\%}$ phytagel was very poor, but that of plantlet grown on the medium with $0.8{\%}$ phytagel was very good.

토양에서 재배되고 있는 묘삼의 뿌리 표피조직에는 근모가 많이 발생하였으나, 배지내에서 생육한 인삼 재분화식물체의 뿌리세포는 표피조직에 근모가 거의 발생하지 않았다. Phytagel이 적게 첨가된 배지에서 자란 유식물체의 뿌리에는 근모가 거의 발생하지 않았으며 근중, 근경, 지근발달 등의 뿌리 발육상태도 미약하였으나, phytagel의 농도가 증가함에 따라 근모의 발달, 근중, 근경, 세근발달 등의 뿌리발육이 양호해지는 것을 관찰할 수 있었다. 또한 phytagel이 적은 배지에서 자란 유식물에서는 통도조직의 발달이 미약하였으나 phytagel이 높은 배지에서 자란 유식물에서는 통도조직의 발달이 아주 양호하였다.

Keywords

References

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