Journal of Biosystems Engineering

  • 제29권6호
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  • Pages.553-557
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  • 2004
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  • 1738-1266(pISSN)
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  • 2234-1862(eISSN)
한국농업기계학회 (Korean Society for Agricultural Machinery)
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μFIA 바이오센서를 이용한 자당 농도 측정

Measurement of Sucrose Concentration Using a μFIA Biosensor

  • Song D. B. (Division of Agricultural Engineering, Gyeongsang National University/Institute of Agricultural & Life Science)
  • 발행 : 2004.12.01
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초록

A microdialysis coupled flow injection amperometric $({\mu}FIA)$ biosensor was calibrated to measure the concentration of sucrose using 11 standard samples from 2 ml to 70 ml of sucrose solution. The output of the sensor increased linearly with an increase in the sucrose concentration with an $r^2$ correlation of 0.99. The amperometric biosensor was then applied to measure the sucrose concentration of 4 commercial samples (Orange and Pineapple juices, Pepsi, Sprite) and the results compared with those by HPLC. Around $20\~30\%$ error was observed in sucrose concentration measurements of the samples analyzed. The sensor has potential in rapid measurement once the calibration is done. Potential for on-line sensing is also discussed.

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참고문헌

  1. Cass, A. E. G. 1990. Biosensors - A Practical Approach. Oxford University Press, New York
  2. Min, R. W., V. Rajendran, N. Larsson, L. Gorton, J. Planas, and B. H. Hagerdal. 1998. Simultaneous monitoring of glucose and L-lactic acid during a fermentation process in an aqueous two-phase system by on-line FIA with microdialysis sampling and dual biosensor detection. Analytica Chimica Acta. 366: 127-135 https://doi.org/10.1016/S0003-2670(97)00726-5
  3. Rocha, I. and E. C. Ferreira. 2002. On-line simultaneous monitoring of glucose and acetate with FIA during high cell density fermentation of recombinant E. coli. Analytica Chimica Acta. 462: 293-304 https://doi.org/10.1016/S0003-2670(02)00347-1
  4. Ruzicka, J. and E. H. Hansen. 1975. Flow injection analysis. Part I. A new concept of fast continuous flow analysis. Analytica Chimica Acta. 78:145 https://doi.org/10.1016/S0003-2670(01)84761-9
  5. Schugerl, K., B. Hitzmann, H. jurgens, T. Kullick, R. Ulber, and B. Weigal. 1996. Challenges in integrating biosensors and FIA for on-line monitoring and control. TIBTECH. 14: 21-31 https://doi.org/10.1016/0167-7799(96)80910-3
  6. Song, D. B. and J. Irudayaraj. 2003. Measurement of glucose concentration using $\mu$FIA biosensor. J. of KSAM. 28(5): 465-468