Antioxidant Activities of Prunus salicina Lind cv. Soldam (Plum) at Different Growth Stages

생육 시기에 따른 피자두(Prunus salicina Lindl. cv. Soldam)의 항산화 효과

  • Yu Mi-Hee (Department of Food Science and Technology, School of Natural Science, Keimyung University) ;
  • Lee Syngook (Department of Food Science and Technology, School of Natural Science, Keimyung University) ;
  • Im Hyo Gwon (Department of Food Science and Technology, School of Natural Science, Keimyung University) ;
  • Kim Hyun Jeong (The Center for Traditional Microorganism Resources, Keimyung University) ;
  • Lee In-Seon (The Center for Traditional Microorganism Resources, Keimyung University)
  • 유미희 (계명대학교 식품가공학과) ;
  • 이승욱 (계명대학교 식품가공학과) ;
  • 임효권 (계명대학교 식품가공학과) ;
  • 김현정 (계명대학교 전통 미생물자원 개발 및 산업화 연구센터) ;
  • 이인선 (계명대학교 전통 미생물자원 개발 및 산업화 연구센터)
  • Published : 2004.09.01

Abstract

This study was performed to evaluate the antioxidant effect of Prunus salicina Lindl. cv. Soldam at different growth stages (sample 1-8). Previous studies shows that this fruits possess hematopoiesis effect, osteoporosis prevention, and antimutagenic effects. Prunus salicina Lindl. cv. soldam was picked in every 5 days from the 40th day before harvesting date for marketing in Gimcheon, Gyeongbuk. The fruits at different growth stages (sample 1-8) were extracted with 60$\%$ acetone and chlorophyll in the extracts was removed. In proximate compositions, the contents of moisture of sample 1, 5, 8 were 88.52, 87.01, 83.56$\%$ ; crude ash were 7.12, 3.35, 3.57$\%$ ; crude protein were 7.52, 5.55, 3.85$\%$ ; crude fat contents were 3.20, 0.99, 5.15$\%$, respectively. The contents of total polyphenols and condensed tannin in the acetone extracts from sample 1, 5, 8 were 10.67, 4.05, 2.57$\%$, and 8.36, 3.11, 1.88$\%$, respectively. The antioxidantive effect of acetone extracts from immature fruits showed strong scavenging effect on DPPH free radicals. The RC$_{50}$ values of the extracts from sample 1, 2 were 2.23, 9.70 $\mu$g/mL, respectively while those of butylated hydroxyanisole (BRA) was 5.25 $\mu$g/mL. The extracts from immature fruits showed over 85$\%$ inhibition on peroxidation of linoleic acid at 100 $\mu$g/mL as determined by both the ferric thiocyanate (FTC) and the thiobarbituric acid (TBA) method.

본 연구에서는 자두류 중 피자두를 생육시기별로 채취하여 총 8종에 대한 일반성분 및 총 페놀성 화합물, 축합형 탄닌 함량을 조사하였으며, DPPH 자유 라디칼 소거능과 linoleic acid에 대한 항산화 효과를 살펴보았다. 피자두 과육의 수분함량 및 아세톤추출물의 조회분, 조단백, 조지방 함량을 분석한 결과, 시료 1, 5, 8번은 각각 수분 88.52$\%$, 87.01$\%$, 83.56$\%$, 조분 7.12$\%$, 3.35$\%$, 3.57$\%$, 그리고 조단백7.52$\%$, 5.55$\%$, 3.85$\%$, 및 조지방 3.20$\%$, 0.99$\%$, 5.l5$\%$로 나타났다. 또한 피자두 아세톤 추출물의 총 폴리페놀 함량은 시료 1, 5, 8번이 각각 10.67$\%$, 4.05$\%$, 2.57$\%$로 나타났으며, 축합형 탄닌 함량은 각각 8.36$\%$, 3.11$\%$, 1.88$\%$로 나타났다. 항산화 효과를 알아보기 위해 DPPH free radical의 소거활성법을 측정한 결과, 피자두 추출물 1과 2의 RC$_{50}$값이 각각 2.23과 9.70 $\mu$g/mL로 가장 높은 소거활성을 보였으며 피자두가완숙될수록 소거활성은 점차적으로 감소하였다. Linoleicacid를 기질로 한 피자두 추출물의 산화방지 효과를 FTC 법과 TBA 법으로 측정한 결과, 초기 지질산화를 나타내는FTC 가에서는 피자두의 미숙과 추출물 처리구의 경우 85$\%$ 이상의 산화 억제율을 보였고, 후기 지질산화에 관여하는TBA 가는 미숙과 추출물에서 85% 이상의 산화 억제율을보였으나 피자두가 완숙될수록 산화 억제율은 감소하였다.

Keywords

References

  1. Gutteridge, J.M.C. and Halliwell, B. (1994) Antioxidants in nutrition, health, and disease. Oxford University Press, p.1-62
  2. Jayat, C., Ratinaud, and M.H. (1993) Cell cycle analysis by flow cytometry : principles and applications. BioI. Cell, 78, 15-25 https://doi.org/10.1016/0248-4900(93)90110-Z
  3. Chance, B., Sies, H. and Boveris A.(I979) Hydroperoxide metabolism in mammmalian organs. Physiol. Rev., 59, 527605
  4. 김숙희, 김화영 (1995) 노화. 대우학술총서, (주)민응사, 서울, p.83-85
  5. Ames, B.N. and Saul, R.L. (1987) Oxidative DNA damage, cancer and aging. Oxygen and human disease. Ann. Inter. Moo., 107, 536-539
  6. Kim, J.H. (1998) Antioxidative activity and phannaco-constituents of houttuyniae herba. M.S. Thesis, Sookmyung Woman's University.-
  7. Shin, U.Y. (1998) Studies en biological activities of spargnanium erectum. PhD. Dessertation, Dongduk woman's University
  8. 황금희, 김현구 (1995) 기능석싱품 소재로서의 생리활성 천영물의 국내 연구 동향. Food Sci. Indudtry, 28, 75-105
  9. Chung, K.H. (1999) Morphological characteristics and principal component analysis of plums. Dept. of Fruit Breeding. National Horticultural Research Institute, R.D.A. Suwon, Korea, p.310-440
  10. Sung, Y.J., Kim, Y.C., Kim, M.Y., Lee, J.B., and Chung, S.K. (2002) Approximate composition and physicochemical properties of plum (Prunus salicina). J. Korean Soc. Agric. Chem. Biotechnol., 45, 134-137
  11. Rice-Evans, C.A. and Diplock, A.T. (1993) Current status of antioxidant therapy. Free radic. Bio. Med., 15, 77-86 https://doi.org/10.1016/0891-5849(93)90127-G
  12. Moritz, K. (1954) New observations on the solubility of prussian blue. Analytica Chemica Acta, 11, 18-27 https://doi.org/10.1016/S0003-2670(00)87685-0
  13. Marja-Riitta, J., Riitta, J. and Ann, E.H. (1996) Salivary tannin-binding proteins in root vole (microtus oeconomus pallas). Biochemical Systematics and Ecology, 24, 25-35 https://doi.org/10.1016/0305-1978(95)00089-5
  14. Blois, M.S. (1977) Antioxidant determinations by the use of stable free radical. J. Agic. Food Chem., 25, 103-107 https://doi.org/10.1021/jf60209a051
  15. Nakatani, N. and Kikuzaki, H.A (1987) New antioxidative glucoside isolated from oregan(origanum vulgare L). Agric. BioI. Chem., 51, 2727-2781 https://doi.org/10.1271/bbb1961.51.2727
  16. Ottolenghi, A. (1959) interacction of ascabic acid mitochondria ipid. Arch. Biochem. biophys., 79, 355-359 https://doi.org/10.1016/0003-9861(59)90414-X
  17. Seo, S.B., Han, S.M., Kim, J.H., Kim, N.M. and Lee, J.S. (2001) Manufacture and physiological functionality of wines and liquors by using plum. Korean J. Biotechnol. Bioeng., 16, 153-157
  18. Yoshizawa, S., Horiuchi, T., Yoshida, T. and Okuda, T. (1987) Antitumor promoting activity of (-)-epigallocatechin gallate, the main constitutent of tannin in green rea. Phytotner. Res., 1, 44-47 https://doi.org/10.1002/ptr.2650010110
  19. Kim, H.J., Jun, B.S., Kim, S.K., Cha, J.Y. and Cho, Y.S. (2000) Polyphenolic compound content and antioxidative activities by extracts from seed, sprout and flower of safflower (Carthamus tinctorius L.). J. Korean Soc. Food Sci. Nutr., 29, 1127-1132
  20. Lee, J.R and Lee, S.R. (1994) Analysis of phenolic substances content in Korean plant foods. Korean J. Food Sci. Technol., 26, 310-316
  21. Lee, Y.C., Hwang, K.H., Han, D.H. and Kim, S.D. (1997) Compositions of opuntia ficus-india. Korean J. Food Sci. Technol., 29, 847-853
  22. Lee, C.H., Chung, M.C., Lee, H.J. and Kho, Y.H. (2000) An apoptosis regulator isolated from petasites japonicum. Korean J. Food Sci. Technol., 32, 448-453