Antimutagenic Effects against Aflatoxin B1 on Culture Conditions of Leuconostoc mesenteroides subsp. cremoris DLAB19 Isolated from Dongchimi

동치미에서 분리한 Leuconostoc mesenteroides subsp. cremoris DLAB19의 배양조건에 따른 Aflatoxin B1에 대한 항돌연변이 효과

  • Rhee Chang-Ho (Department of Food Science and Technology, Kyungpook National University) ;
  • Kim Jung-Hee (Department of Food and Beverage Culinary, Kyungdong Collage of Techno-Information.) ;
  • Park Heui-Dong (Department of Food Science and Technology, Kyungpook National University)
  • 이창호 (경북대학교 식품공학과) ;
  • 김정희 (경동정보대학 식음료조리과) ;
  • 박희동 (경북대학교 식품공학과)
  • Published : 2004.09.01

Abstract

Leuconostoc mesenteroides subsp. cremoris DLAB19 were investigated under various culture conditions to maximize the production of antimutagenic substance(s) against aflatoxin Bl(AFBl) on Salmonella enterica serovar Typhimurium TAI00 and TA98. The MRS medium containing glucose(2$\%$) as a carbon source and yeast extract(1 $\%$) as a nitrogen source resulted in the highest production of the antimutagenic substance(s) against aflatoxin Bl(AFBl) in the culture supernatant of Leu. mesenteroides subsp. cremoris DLAB19. Optimal pH of the medium, culture temperature and shaking speed for the antimutagenic substance(s) production were pH 7.0, 30$^{\circ}C$ and 150 rpm, respectively. Under the optimal condition, the antimutagenic effects of Leu. mesenteroides subsp. cremoris DLAB19 culture supernatant were 87.11 $\%$ on S. enterica serovar Typhimurium TA100 and 75.04 S. enterica serovar Typhimurium TA98.

동치미에서 분리한 유산균인 Leu. mesenteroides subsp.cremoris DLAB19 균주의 aflatoxin Bl에 대한 항 돌연변이원성 물질 생산을 위한 최적 조건을 조사한 결과, 탄소원으로 glucose를 첨가시 가장 높은 항 돌연변이 효과를 나타내었으며, 질소원으로서는 yeast extract 첨가시 항 돌연변이 효과가 우수하였다. 탄소원으로 glucose의 농도을 2$\%$ 첨가시aflatoxin Bl에 대한 항 돌연변이 효과가 가장 우수하였으며,질소원으로서 yeast extract 1$\%$ 첨가시 가장 우수한 항 돌연변이 효과를 나타내었다. 항 돌연변이원성 물질 생산을 위한 최적 배양 조건은 초기 pH, 배양 온도, 진탕 속도가 각각 7.0, 30$^{\circ}C$ 및 150 rpm이었다. 상기의 최적 조건에서 36시간 배양시 가장 높은 항 돌연변이 효과를 나타내었는데 S. enterica serovar Typhimurim TA100과 TA98을 이용한 경우항 돌연변이 효과가 87.11$\%$와 75.04$\%$이었다.

Keywords

References

  1. Carr, J.G. (1975) Lactic acid bacteria in Beverage and Foods. Academic press, New York, p.50-79
  2. Kada, T., Kaneko, K., Matsuzaki, T. and Hara, Y. (1985) Detection and chemiml identification of natural bio-antimutagenic: A case of green tea factor. Mutat Res., 150, 127-132 https://doi.org/10.1016/0027-5107(85)90109-5
  3. Adachi, S. (1992) Lactic acid bacteria and the control of tumors, In B. J. B. Wood(ed), the Lactic Acid Bacteria Vol. 1: The Lactic Acid Bacteria in Health and Diease. Elsevier Applied Science, London, p.233-247
  4. Fernandes, C.F., Shahni, K.M and Amer, M.A. (1987) Therapeutic role of dietary and Lactobacilli fermented dairy products. FEMS Microbiol. Rev., 46, 343-356 https://doi.org/10.1111/j.1574-6968.1987.tb02471.x
  5. Fernandes, C.F., Shahni, K.M. and Amer, M.A. (1988) Control of diarrhea by Lactobacilli. J. Appl. Nutr., 40, 32-39
  6. Gilland, S.E. (1989) Acidophilus milk products: A review of potential benefits to consumer. J. Dairy Sci., 72, 2483-2489 https://doi.org/10.3168/jds.S0022-0302(89)79389-9
  7. Gilland, S.E., Speck, M.I. and Nauyok, G.F. (1978) In fluence of consuming nonfermented milk containing Lactobacillus acidophilus on fecal flora healthy males. J. Dairy Sci., 61, 1-7 https://doi.org/10.3168/jds.S0022-0302(78)83543-7
  8. Gloden, B.R. and Gorbach, S.L. (1984) The effect of milk and Lactobacillus feeding on human intestinal bacterial enzyme activity. Am. J. Clin. Nutr., 39, 756-762
  9. Shahani, K.M. and Chandan, R.C. (1979) Nutritional and healthful aspects of cultured and culture-containing dairy foods. J. Dairy Sci., 62, 2884-2889
  10. Shun, Y.L., Ayres, J.A., Winkler, W. and Sandine, W.E. (1989) Lactobacillus effect on cholesterol: In vitro and in vivo results. J. Dairy Sci., 72, 2884-2889
  11. Deeth, H.C. (1984) Yogurt and cultured products. Aust. J. Dairy Technol., 39, 111-1l4
  12. Savino, D.A. and Levitt, M.D. (1987) Milk intolerance and microbe-containing dairy foods. J. Dairy Sci., 70, 397-403 https://doi.org/10.3168/jds.S0022-0302(87)80023-1
  13. Robinsin, R.K. (1989) Special yogurts the potential health benefits. Dairy Ind. Int., 54, 23-29
  14. Kinoshita, N. and Gelcoin, H.V. (1978) $\beta$-glucuronidase catalyzed hydrolysis of benzo($\alpha$)pyrene-3-glucuronide and binding to DNA. Science, 199, 307-314 https://doi.org/10.1126/science.619459
  15. Laqueur, G.L. and Spatz, M. (1975) Oncogenicity of cycasin and methylazoxymethanol. Cancer Res., 17, 189-196
  16. Macdonald, l.A, Bussated, R.G., Hutdhinson, D.M. and Holdelman, L.V. (1984) Rutin-induced $\beta$-glucuronidase activity in Streptococcus faecium VGH-l and Streptococcus sp. strain FRP-17 isolated from human feces. Appl. Environ. Microbiol., 47, 350-357
  17. Fernandes, C.F. and Shahani, K.M. (1990) Anticarciongenic and immunological properties of dietary lactobacilli. J. Food Prot., 53, 704-710
  18. Kato, T., Yokokawa, T. and Mutai, N. (1984) Augmentation of mouse natural killer cell activity by Lactobacillus casei and its surface antigens. Microbiol. Immunol., 28, 209-217
  19. Kato, T., Yokokawa, T. and Mutai, N. (1985) Introduction of tumoricidal peritioneal exudate cells by adminstration of Lactobacillus casei. Inter. J. Immunopharmacol., 7, 103-113 https://doi.org/10.1016/0192-0561(85)90015-3
  20. Perdigon, G., Alvarez, S., Nadar, N.E., de Macias, M.E. and Medici, M. (1989) Effect of lactic acid bacteria orally adminstration and of yoghurt on the immune system. In: Les lactis ferments, Actualite de la recherche. John Libbery Eurotext Ltd. Oaris, p.77-84
  21. Perdigon, G., Nacer, W.E., Alvarez, S., Oliver, G. and de R. Goigado, P. (1988) Sysrematic augmentation of the immune response in mice feeding fermented milks with Lactobacillus casei and Lactobacillus acidophilus. Immunol., 63, 17-24
  22. Perdigon, G., de Macias, M.E.N., Alvarez, S., Medici, G., Oliver, M. and de Ruiz Holgado, A.P. (1986) Effect of a mixture of Lactobacillus casei and Lactobacillus acidophilus administered orally on the immune system in mice. J. Food Prot.. 49. 986-989
  23. Ayebo, A.D., Shahani, K.M. and Dam, R. (1981) Antitumor component of yogurt fractionation. J. Dairy Sci., 64, 2318-2323 https://doi.org/10.3168/jds.S0022-0302(81)82853-6
  24. Ayebo, A.D., Shahani, K.M. and Dam, R (1982) len exchange seperation of the antitumor component of yogurt dialyzate. J. Dairy Sci., 65, 2388-2394 https://doi.org/10.3168/jds.S0022-0302(82)82513-7
  25. Reddy, G.V., Shahani, K.M. and Banerjee, M.R. (1973) Inhibitory effect of yoghurt on Ehrlich ascites tumor-cell proliferation. J. Natl. Cancer Inst., 63, 17-24
  26. Kang, K.O., Sohn, H.J. and Kim, W.J. (1991) Changes in chemical and sensory properties of Dongchimi during fermentation. Kor. J. Food Sci. Technol., 23, 267-271
  27. Maron, D.M. and Ames, B.N. (1983) Revised methods for the Salmonella mutagenicity test. Mutat. Res., 113, 173-219 https://doi.org/10.1016/0165-1161(83)90010-9
  28. Yahagi, T., Degawa, M., Seino, Y., Matsushima, T., Nagao, M., Sugimura, T. and Hashimoto, Y. (1975) Mutagenicity of carcinogenic azo dyes and their derivatives. Cancer Lett., 1, 91-98 https://doi.org/10.1016/S0304-3835(75)95563-9
  29. Yahagi, T., Nagao, M., Seino, Y., Matsushima, T, Sugimura, T. and Okada, M. (1977) Mutagenicities of N-nitrosarnines on Salmonella. Mutat. Res., 48, 121-126 https://doi.org/10.1016/0027-5107(77)90151-8