Antimetastatic Effects of Capsaicin in Murine B16 Melanoma Cell Lines

  • Hwang Myung Sil (National Institute of Toxicological Research, Korea Food and Drug Administration) ;
  • Yum Young Na (National Institute of Toxicological Research, Korea Food and Drug Administration) ;
  • Kang Ho Il (National Institute of Toxicological Research, Korea Food and Drug Administration) ;
  • Kim Ok Hee (National Institute of Toxicological Research, Korea Food and Drug Administration)
  • Published : 2005.03.01

Abstract

The present work was undertaken to evaluate the antimetastatic potential of capsaicin (8­methyl-N-vanillyl-6-nonenamide) by measuring its effects on matrix metalloproteinase activity, cell invasion and lung metastasis. Significant inhibition of matrix metalloproteinase-2 activity by capsaicin (100 $\mu$M) was detected by gelatin zymography. In vitro invasion assay showed capsaicin (50, 100 $\mu$M) reduced tumor cell invasion ($28-40\%$). Capsaicin (i.p., 2.5 mg/kg) inhibited development of lung colonization ($58\%$). These results suggest that capsaicin prevents metastasis in part through suppression of invasion of B16F10 melanoma cells by inhibiting matrix metalloproteinase-2 responsible for degradation of extracellular matrix.

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