Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Cloning of celC, Third Cellulase Gene, from Pectobacterium carotovorum subsp. carotovorum LY34 and its Comparison to Those of Pectobacterium sp.

  • LIM WOO JIN (Division of Applied Life Science, Gyeongsang National University) ;
  • RYU SUNG KEE (Division of Applied Life Science, Gyeongsang National University) ;
  • PARK SANG RYEOL (National Institute of Agricultural Biotechnology, RDA) ;
  • KIM MIN KEUN (Division of Applied Life Science, Gyeongsang National University) ;
  • AN CHANG LONG (Division of Applied Life Science, Gyeongsang National University) ;
  • HONG SU YOUNG (Division of Applied Life Science, Gyeongsang National University) ;
  • SHIN EUN CHULE (Division of Applied Life Science, Gyeongsang National University) ;
  • LEE JONG YEOUL (National Institute of Agricultural Biotechnology, RDA) ;
  • LIM YONG PYO (Department of Horticulture, Chungnam National University) ;
  • YUN HAN DAE (Division of Applied Life Science and Research Institute of Life Science, Gyeongsang National University)
  • Published : 2005.04.01
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Abstract

Phytopathogenic Pectobacterium carotovorum subsp. carotovorum (Pcc) LY34 secretes multiple isozymes of the plant cell wall degrading enzyme endoglucanases. We have cloned a third cel gene encoding CMCase from Pcc LY34. The structural organization of the celC gene (AY188753) consisted of an open reading frame (ORP) of 1,116 bp encoding 371 amino acid residues with a signal peptide of 22 amino acids within the NH$_2$-terminal region of pre-CelC. The predicted amino acid sequence of CelC was similar to that of Peetobaeterium ehrysanthemi Cel8Y (AF282321). The CelC has the conserved region of the glycoside hydrolase family 8. The apparent molecular mass of CelC was calculated to be 39 kDa by CMC-SDS-PAGE. The cellulase­minus mutant of Pee LY34 was as virulent as the wild-type in pathogenicity tests on tubers of potato. The results suggest that the CelC of Pce LY34 is a minor factor for the pathogenesis of soft-rot.

Keywords

References

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