Korean Journal of Environmental Biology (환경생물)

Korean Society of Environmental Biology (한국환경생물학회)
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Cell-type-specific Gene Expression Patterns in Human Carcinoma Cells followed by Irradiation

방사선에 의한 암세포주 특이적 유전자 발현 양상

  • Park Ji-Yoon (Department of Biochem & Mol. Biol, Hanyang University) ;
  • Kim Jin-Kyu (RI Radiation Research Team, Korea Atomic Energy Research Institute) ;
  • Chai Young Gyu (Department of Biochem & Mol. Biol, Hanyang University)
  • 박지윤 (한양대학교 생화학 및 분자생물학과) ;
  • 김진규 (한국원자력연구소 동위원소 방사선 응용팀) ;
  • 채영규 (한양대학교 생화학 및 분자생물학과)
  • Published : 2005.06.01
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Abstract

Ionizing radiation is a well- known therapy factor for human carcinoma cells. Genotoxic stress mediates cell cycle control, transcription and cellular signaling. In this work, we have used a microarray hybridization approach to characterize the cell type-specific transcriptional response of human carcinoma MCF-7 and HeLa cell line to $\gamma-radiation$, such as 4Gy 4hr. We found that exposure to $\gamma-ray$ alters by at least a $log_2$ factor of 1.0 the expression of known genes. Of the 27 genes affected by irradiation, 11 are down- regulated in MCF-7 cells and 2 genes induced by radiation,15 are repressed in HeLa cells. Many genes were involved in known damage- response pathways for cell cycling, transcription factor and cellular signaling response. However, in MCF-7 cells, we observed gene expression pattern in chromatin, apoptosis, stress, differentiation, cytokine, metabolism, ribosome and calcium. In HeLa cells, it showed clearly the expression changes in adhesion and migration, lysosome, brain, genome instability and translation. These insights reveal new therapy directions for studying the human carcinoma cell response to radiation.

Keywords

References

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