Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Effects of Acorn (Quercus acutissima CARR.) Supplementation on the Level of Acetylcholine and Its Related Enzyme Activities in the Brain of Dementia Mouse Model

도토리 급여가 치매모델 마우스 뇌조직의 아세틸콜린 및 관련효소 활성에 미치는 영향

  • Lee, Sung-Hyeon (National Rural Resources Development Institute, NIAST, RDA) ;
  • Kim, Dae-Ik (National Rural Resources Development Institute, NIAST, RDA) ;
  • Cho, So-Yong (National Rural Resources Development Institute, NIAST, RDA) ;
  • Jung, Hyun-Jin (National Rural Resources Development Institute, NIAST, RDA) ;
  • Cho, Soo-Muk (National Rural Resources Development Institute, NIAST, RDA) ;
  • Park, Hong-Ju (National Rural Resources Development Institute, NIAST, RDA) ;
  • Lillehoj Hyun S. (Animal and Natural Resources institute, ARS- USDA)
  • 이성현 (농촌진흥청 농업과학기술원 농촌자원개발연구소) ;
  • 김대익 (농촌진흥청 농업과학기술원 농촌자원개발연구소) ;
  • 조소영 (농촌진흥청 농업과학기술원 농촌자원개발연구소) ;
  • 정현진 (농촌진흥청 농업과학기술원 농촌자원개발연구소) ;
  • 조수묵 (농촌진흥청 농업과학기술원 농촌자원개발연구소) ;
  • 박홍주 (농촌진흥청 농업과학기술원 농촌자원개발연구소) ;
  • Published : 2005.06.01
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Abstract

This study was designed to investigate the anti-dementia effects of acorn (Quercus acutissima CARR.) in brain of the mouse. Dementia model was induced by administration of scopolamin (30 mg/kg BW) Male ICR mouse $(30{\pm}2g\;BW)$ were fed basal diet (control group), and experimental diets (AP-5 and AP-10 groups) added $5\%\;and\;10\%$ of dried acorn powder to basal diet for 8 months. Acetylcholine content significantly increase in AP-5 and AP-10 groups ($4.2\%\;and\;11.3\%$, respectively) compared with control group. Acetylcholinesterase activities were significantly inhibited ($13.5\%\;and\;17.6\%$, respectively) in brain of AP-5 and AP-10 groups. Monoamine oxidase-B (MAO-B) activities were significantly inhibited ($10.0\%\;and\;12.7\%$, respectively) in brain of AP-S and AP-10 groups. These results suggest that acorn (Q. acutissima CARR.) may play an effective role in an attenuating various age-related changes such as dementia including learning and memory impairments in brain.

고령화 현상에 의한 노인 인구의 증가에 따라 노인성 질환연구의 중요성이 대두되고 있으며, 특히 노인성 질환 중 가장 문제되는 치매(dementia)에서 노인성 치매(Alzhemier's disease)는 복합적 인 원인에 의 해서 나타나므로 다양한 관점에서의 연구가 필요하다. 따라서 본 연구에서는 마우스에 scopolamin(30 mg/kg BW)을 투여하여 기억${\cdot}$학습장애를 유발하고, 뇌조직의 아세틸콜린 및 그 관련 효소에 미치는 도토리 분말의 영향을 분석하였다. 뇌의 가장 중요한 신경전달 물질로서 아세틸콜린 생성에 미치는 도토리 분말 투여효과를 비교하였을 때, AP-5및 AP-10 투여군에서 $4.2\%$ 및 $11.3\%$의 ACh 합성이 증가하는 경향이 나타났으며, ACh의 분해효소인 acetylcholinesterase(AChE)의 활성은 AP-5 및 AP-10의 도토리 분말 투여군에서 $13.5\%$ 및 $17.6\%$로서 AChE효소활성이 유의적으로 감소하였다. 그리고 카테롤아민계 신경전달물질의 파괴에 관계하는MAO-B효소의 활성은 AP-5 및 AP-10의 도토리 분말 투여군에서 $10.0\%$ 및 $12.7\%$로서 도토리 분말 투여에 의해 MAO-B효소활성이 현저하게 억제되었다. 따라서 도토리는 AChE 효소의 활성을 저해하여 ACh의 농도를 높일 뿐만 아니라 MAO-B효소의 활성을 효과적으로 억제하여 카테콜아민계 신경전달물질을 잘 보존함으로써 치매 예방에 도움이 될 것으로 기대된다.

Keywords

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