Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
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Effects of Citrus Reticulata on the Cell Detachment and Apoptosis in Human Gastric Cancer SNU-668 Cells

  • Kim, Jeung-Beum (Department of Oriental Pathology, College of Oriental Medicine, Semyung University) ;
  • Kim, Min-Su (Department of Oriental Pathology, College of Oriental Medicine, Semyung University) ;
  • Kim, Ee-Hwa (Department of Acupoint & Meridian, College of Oriental Medicine, Semyung University) ;
  • Kim, Sung-Hoon (Chemoprevention Lab, Graduate School of East-West Medical Science, Kyunghee University)
  • Published : 2005.02.25
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Abstract

The purpose of this study was to examine the effects of Citrus Reticulata(CR) on the Cell Detachment and Apoptosis in Human Gastric Cancer SNU-668 Cells. The effect of CR on apoptosis was investigated through MTT assay, DAPI staining, and TUNEL assay. We also performed RT-PCR for apoptotic genes including BCL-2, BAX, and caspase-3, the caspase-3 activity assay, and western blotting for pro-CASP-3. Then, to detect that adhesion of cell to ECM was reduced by CR, we investigated mRNA expression of CDH1 and PTK2 using RT-PCR, and their protein expressions using western blotting, and immunocytochemistry in SNU-668 cells. In this study, the results showed that treatment of CR induced time and dose-dependent cell death in SNU-668 cells. Downregulated mRNA expression of BCL-2, and upregulated mRNA expressions of BAX and CASP-3 indicated that the cell death was due to apoptosis. Protein expression of inactivated CASP-3, and caspase-3 activity assay also showed that apoptosis was induced in CR-treated cells.

Keywords

References

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